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• W2051874486 abstract "The characteristic fragmentation of acetyl derivatives or underivatives in reverse-phase high-performance liquid chromatography–thermospray-mass spectrometry (RPLC–TSP-MS) analysis were investigated, and the utility of RPLC–TSP-MS analysis as a basal method for the detection of oxidation products of polyunsaturated-fatty acids (PUFAs) was reviewed. In polyhydroxy-PUFAs, the acetyl derivatives were suitable for RPLC–TSP-MS analysis, since high-stability in the sensitivity by a single mobile phase and high-sensitivity by strong base ion, [MH−n(60)]+ (n=number of the hydroxy groups), based on the elimination of acetic acid (60 mass units) from MH+ ion without other-fragmentation were obtained. In hydroperoxy-PUFAs, the acetyl derivatives were suitable for RPLC–TSP-MS analysis, since strong base ions, [MH−60]+ and [MH−60−H2O]+, were obtained, and the values of m/z differed from the value of m/z from the corresponding hydroxy-PUFA. For fractional determination of underived ω-hydroxy-PUFAs, the characteristics of the high-intensity of the adduct ion (MH+, MNH4+, MNa+) in RPLC–TSP-MS analysis were utilized, since other common monohydroxy-PUFAs were characterized by the high-intensity of [MH−H2O]+ ion. For fractional determination of underived epoxy-PUFAs, epoxyhydroxy-PUFAs and 5,6-dihydroxyeicosatrienoate-1,5-lactone (5,6-DiHETriE-δ-lactone), the characteristics of the high-intensity of the adduct ion (MH+, MNH4+, MNa+) in RPLC–TSP-mass spectra were utilized. Then, the fragment ions formed by cleavage of two-types in the oxirane ring of epoxy-PUFA under electron-beam on conditions provided information as to the position of the oxirane ring." @default.
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• W2051874486 date "2002-08-01" @default.
• W2051874486 modified "2023-10-18" @default.
• W2051874486 title "High-performance liquid chromatography–thermospray ionization-mass spectrometry of the oxidation products of polyunsaturated-fatty acids" @default.
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• W2051874486 doi "https://doi.org/10.1016/s0003-2670(01)01443-x" @default.
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