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- W2051885257 abstract "A simple method has been developed to prepare silica-encapsulated Ag (Ag@SiO2) Raman tags, embedded with the reporter molecules 4-mercaptobenzoic acid (MBA), using environmental-friendly solvents (mixed solvents of ethanol and water) without vitreophilic pretreatment. The Ag@SiO2 Raman tags exhibited excellent stability resistance to high-concentration salts and long-term storage. Sensitivity of the Ag@SiO2 SERS tags could be enhanced by increasing MBA coverage and reducing the silica shell thickness, and the Ag@SiO2 SERS tags had versatility for bioassays. Myoglobin (Mb)-conjugated Ag@SiO2 Raman tags specifically bound to iminodiacetic acid (IDA)-functionalized wafers through the coordination interactions of IDA–Cu2+–histidine residues available on the Mb surfaces for SERS detection. Mb could be directly detected by means of the formation of the Mb-sandwiched structures via Cu2+ coordination between the IDA-functionalized wafers and IDA-modified Ag@SiO2–Au composite nanoparticles, where Au nanoparticles were adsorbed onto aminated Ag@SiO2 Raman tags followed by modifying with IDA. It is clear that the prepared Ag@SiO2 Raman tags can be applied for assays of other proteins or biomolecules if corresponding specific ligands are used and have potential application in immunoassays." @default.
- W2051885257 created "2016-06-24" @default.
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- W2051885257 date "2012-01-01" @default.
- W2051885257 modified "2023-10-09" @default.
- W2051885257 title "Synthesis and application of surface enhanced Raman scattering (SERS) tags of Ag@SiO2 core/shell nanoparticles in protein detection" @default.
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- W2051885257 doi "https://doi.org/10.1039/c2jm16397g" @default.
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