FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051942007> ?p ?o ?g. }

• W2051942007 endingPage "1158" @default.
• W2051942007 startingPage "1149" @default.
• W2051942007 abstract "Incubation of human eosinophils in BSA-coated tissue culture plates resulted in time-dependent adhesion and attendant activation of the NADPH oxidase that were both inhibited (by >85%) by blocking antibodies raised against CD11b and CD18. SB 203580, an inhibitor of p38 mitogen-activated protein (MAP) kinase, did not influence adhesion but inhibited superoxide anion generation (pIC50=−6.57). PP1, an inhibitor of the src-family of protein tyrosine kinases, inhibited adhesion and CD11b/CD18-mediated superoxide anion generation with similar potencies (pEC50s=−5.53 and −5.99 respectively) suggesting that inhibition of the NADPH oxidase was a direct consequence of blocking adhesion. The protein kinase C (PKC) inhibitors Ro-31 8220 (broad spectrum inhibitor), GF 109203X (inhibitor of conventional and novel isoforms) and Gö 6976 (inhibitor of conventional isoforms) suppressed adhesion-dependent NADPH oxidase activation (pIC50s=−6.61, −6.05 and −4.89 respectively) without affecting adhesion. Based upon the selectivity of these drugs PKCδ and PKCε are implicated in the suppression of oxidant production. Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PtdIns 3-kinase), abolished superoxide anion production in adherent eosinophils (pEC50=−9.06). Similarly, CD11b/CD18-dependent adhesion was suppressed with the same potency (pEC50=−9.29) although the maximum effect did not exceed 50% implying that wortmannin also had an affect on those processes that govern adhesion-driven oxidase activation. PD 098059 and piceatannol, inhibitors of MAP kinase kinase-1 and the syk tyrosine kinase respectively, had no effect on CD11b/CD18-mediated adhesion or NADPH oxidase activation. The results of this study demonstrate that human eosinophils adhere to BSA-coated plastic by a CD11b/CD18-dependent mechanism, which is responsible for activation of the NADPH oxidase. Although the signalling pathway(s) utilized by CD11b/CD18 is still to be elucidated, the data presented herein implicate p38 MAP kinase, novel PKCs and PtdIns 3-kinase. British Journal of Pharmacology (1999) 128, 1149–1158; doi:10.1038/sj.bjp.0702892" @default.
• W2051942007 created "2016-06-24" @default.
• W2051942007 creator A5018753336 @default.
• W2051942007 creator A5030258071 @default.
• W2051942007 creator A5040482894 @default.
• W2051942007 creator A5067343618 @default.
• W2051942007 creator A5071605337 @default.
• W2051942007 date "1999-11-01" @default.
• W2051942007 modified "2023-10-14" @default.
• W2051942007 title "‘Outside-in’ signalling mechanisms underlying CD11b/CD18-mediated NADPH oxidase activation in human adherent blood eosinophils" @default.
• W2051942007 cites W1492927281 @default.
• W2051942007 cites W1497341558 @default.
• W2051942007 cites W1510633031 @default.
• W2051942007 cites W1525696296 @default.
• W2051942007 cites W1538637439 @default.
• W2051942007 cites W1572415265 @default.
• W2051942007 cites W1576671418 @default.
• W2051942007 cites W1583256888 @default.
• W2051942007 cites W1587932799 @default.
• W2051942007 cites W1641731911 @default.
• W2051942007 cites W1660988713 @default.
• W2051942007 cites W1766953595 @default.
• W2051942007 cites W180810881 @default.
• W2051942007 cites W1818313483 @default.
• W2051942007 cites W1896539662 @default.
• W2051942007 cites W1924609456 @default.
• W2051942007 cites W1965321622 @default.
• W2051942007 cites W1965671230 @default.
• W2051942007 cites W1966103571 @default.
• W2051942007 cites W1970612407 @default.
• W2051942007 cites W1971915141 @default.
• W2051942007 cites W1973436145 @default.
• W2051942007 cites W1980048339 @default.
• W2051942007 cites W1981124481 @default.
• W2051942007 cites W1987263036 @default.
• W2051942007 cites W2000108972 @default.
• W2051942007 cites W2002549957 @default.
• W2051942007 cites W2015648136 @default.
• W2051942007 cites W2024391009 @default.
• W2051942007 cites W2027266132 @default.
• W2051942007 cites W2030196438 @default.
• W2051942007 cites W2034978654 @default.
• W2051942007 cites W2035936653 @default.
• W2051942007 cites W2036270164 @default.
• W2051942007 cites W2036946165 @default.
• W2051942007 cites W2043206871 @default.
• W2051942007 cites W2045074115 @default.
• W2051942007 cites W2052954853 @default.
• W2051942007 cites W2058127710 @default.
• W2051942007 cites W2069288734 @default.
• W2051942007 cites W2069720072 @default.
• W2051942007 cites W2083095157 @default.
• W2051942007 cites W2085313845 @default.
• W2051942007 cites W2086524338 @default.
• W2051942007 cites W2088525733 @default.
• W2051942007 cites W2090895449 @default.
• W2051942007 cites W2092657066 @default.
• W2051942007 cites W2106601033 @default.
• W2051942007 cites W2115338907 @default.
• W2051942007 cites W2116067317 @default.
• W2051942007 cites W2124962639 @default.
• W2051942007 cites W2133527571 @default.
• W2051942007 cites W2153883530 @default.
• W2051942007 cites W2157952919 @default.
• W2051942007 cites W2158239018 @default.
• W2051942007 cites W2163635139 @default.
• W2051942007 cites W2164241215 @default.
• W2051942007 cites W2168203007 @default.
• W2051942007 cites W2169125588 @default.
• W2051942007 cites W2282042267 @default.
• W2051942007 cites W2292202903 @default.
• W2051942007 cites W2337313478 @default.
• W2051942007 cites W2404282203 @default.
• W2051942007 cites W2404946630 @default.
• W2051942007 cites W2414685758 @default.
• W2051942007 cites W2418712387 @default.
• W2051942007 cites W4313333755 @default.
• W2051942007 cites W6580817 @default.
• W2051942007 doi "https://doi.org/10.1038/sj.bjp.0702892" @default.
• W2051942007 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1571741" @default.
• W2051942007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10578126" @default.
• W2051942007 hasPublicationYear "1999" @default.
• W2051942007 type Work @default.
• W2051942007 sameAs 2051942007 @default.
• W2051942007 citedByCount "51" @default.
• W2051942007 countsByYear W20519420072012 @default.
• W2051942007 countsByYear W20519420072013 @default.
• W2051942007 countsByYear W20519420072016 @default.
• W2051942007 countsByYear W20519420072017 @default.
• W2051942007 countsByYear W20519420072018 @default.
• W2051942007 crossrefType "journal-article" @default.
• W2051942007 hasAuthorship W2051942007A5018753336 @default.
• W2051942007 hasAuthorship W2051942007A5030258071 @default.
• W2051942007 hasAuthorship W2051942007A5040482894 @default.
• W2051942007 hasAuthorship W2051942007A5067343618 @default.
• W2051942007 hasAuthorship W2051942007A5071605337 @default.
• W2051942007 hasBestOaLocation W20519420071 @default.
• W2051942007 hasConcept C153911025 @default.

• next