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- W2051947731 abstract "Knowledge of the stability of radioiodinated MoAb and their fragments in vitro is necessary for their practical application and commercialization. Using molecular-sieving (TSK-3000) high performance liquid chromatography (HPLC), cellulose acetate electrophoresis (CAE) at 11 and 45 minutes and solid phase immunoreactivity, we have observed the in vitro stability of a B cell lymphoma antibody (Lym-1, IgG2a) and its Fab fragment, and a mammary cancer antibody (B6.01, IgG1) and its F(ab‘)2 fragment. Each species was iodinated (I-125) with chloramine-T (C-T) at one microgram C-T per microgram of MoAb and one atom of iodine per molecule of MoAb, and were stored at 4°C. All four species showed mild degradation by 3 days, but this was only slowly progressive for the whole antibodies up to the final observation time of 21 days. F(ab’)2 more rapidly degraded between 6 and 14 days, and Fab between 3 and 6 days, as well as subsequently. The results by HPLC and CAE were comparable and were confirmed by tests of immunoreactivity. In summary, there was no significant difference in degradation in vitro between an IgG2a and an IgGl antibody iodinated in an identical manner, and these molecules were reasonably stable for 14–21 days at 4°C. Fragments of these MoAb iodinated in an identical manner were less stable, and this seemed to be size-related. This could be a reflection of the relatively greater impact of the addition of an atom of iodine to a smaller species, but more likely reflects an inherently reduced stability of the fragments." @default.
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- W2051947731 date "1984-09-01" @default.
- W2051947731 modified "2023-10-14" @default.
- W2051947731 title "STABILITY OF RADIOIODIMATED MONOCLONAL ANTIBODIES (MoAb) AND THEIR F(ab')2 AND Fab FRAGMENTS IN VITRO" @default.
- W2051947731 doi "https://doi.org/10.1097/00003072-198409001-00036" @default.
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