FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051998700> ?p ?o ?g. }

• W2051998700 endingPage "213" @default.
• W2051998700 startingPage "204" @default.
• W2051998700 abstract "We previously demonstrated that all-trans-retinoic acid (all-trans-RA) regulates gonadotropin-releasing hormone (GnRH) release and gene expression in rat hypothalamic fragments and GT1-1 neuronal cells. Promoter analysis of rat GnRH gene revealed that the enhancing effect of all-trans-RA on GnRH transcription is mediated by cis-elements localized within −1640/−1438 of the rat GnRH promoter. In the present study, we attempted to localize functional retinoic acid response elements (RAREs) within the all-trans-RA-responsive region of the rat GnRH gene. Sequence analysis showed that there exist three putative repeats of AGGTCA-related sequences (−1637/−1617, −1579/−1562, and −1494/−1470) within this promoter sequence. Among them, only the −1494/−1470 sequence could compete the specific binding of GT1-1 nuclear extracts to the consensus RARE (direct repeat of AGGTCA with a 5-bp spacer, DR-5) and vice versa in electrophoretic mobility shift assays. In addition, like consensus RARE, the −1494/−1470 sequence could confer all-trans-RA responsiveness when inserted into the upstream region of SV40 promoter. Treatment of GT1-1 cells with all-trans- or 9-cis-RA increased the specific bindings of GT1-1 nuclear extracts to the consensus RARE and to the −1494/−1470 sequence while not affecting the specific binding to the cAMP response element (CRE). Both retinoids induced RARβ gene expression in GT1-1 cells. The −1494/−1470 sequence (5′-TCTTAGGACTCTGTGTGACCTAAGA) is similar to the direct repeat of TGACCT (complementary sequence of AGGTCA) with a spacer of 5 bp (i.e. DR-5 in the reverse orientation). A mutation of the second core recognition motif of the −1494/−1470 sequence to a more divergent one from consensus RARE (from TGACCT to TTACAT) abolished the responsiveness to all-trans-RA, whereas a mutation of first core recognition motif to a more TGACCT-like sequence (from AGGACT to TGAACT) increased the responsiveness to all-trans-RA. These results indicate that the −1494/−1470 sequence is indeed a weak but functional RARE of the modified DR-5 type. Taken together, these data indicate that all-trans-RA enhances GnRH transcription via functional RARE present in the distal region of the GnRH promoter." @default.
• W2051998700 created "2016-06-24" @default.
• W2051998700 creator A5026360578 @default.
• W2051998700 creator A5035227436 @default.
• W2051998700 creator A5035243568 @default.
• W2051998700 creator A5048302807 @default.
• W2051998700 creator A5060429660 @default.
• W2051998700 creator A5060485274 @default.
• W2051998700 creator A5070846276 @default.
• W2051998700 date "2001-03-01" @default.
• W2051998700 modified "2023-10-03" @default.
• W2051998700 title "A functional retinoic acid response element (RARE) is present within the distal promoter of the rat gonadotropin-releasing hormone (GnRH) gene" @default.
• W2051998700 cites W1493100105 @default.
• W2051998700 cites W1564556915 @default.
• W2051998700 cites W1587588231 @default.
• W2051998700 cites W1797193362 @default.
• W2051998700 cites W1841957993 @default.
• W2051998700 cites W1900719576 @default.
• W2051998700 cites W1927684145 @default.
• W2051998700 cites W1939266489 @default.
• W2051998700 cites W1949708034 @default.
• W2051998700 cites W1972375217 @default.
• W2051998700 cites W1973207223 @default.
• W2051998700 cites W1981378049 @default.
• W2051998700 cites W1988366048 @default.
• W2051998700 cites W1992663511 @default.
• W2051998700 cites W1993243592 @default.
• W2051998700 cites W1995754186 @default.
• W2051998700 cites W1997818599 @default.
• W2051998700 cites W1999352792 @default.
• W2051998700 cites W2011467252 @default.
• W2051998700 cites W2014956497 @default.
• W2051998700 cites W2017490900 @default.
• W2051998700 cites W2039096763 @default.
• W2051998700 cites W2041781038 @default.
• W2051998700 cites W2051663421 @default.
• W2051998700 cites W2052594397 @default.
• W2051998700 cites W2057745494 @default.
• W2051998700 cites W2062138734 @default.
• W2051998700 cites W2063645101 @default.
• W2051998700 cites W2066055985 @default.
• W2051998700 cites W2075451661 @default.
• W2051998700 cites W2078790265 @default.
• W2051998700 cites W2081893687 @default.
• W2051998700 cites W2088430609 @default.
• W2051998700 cites W2090615253 @default.
• W2051998700 cites W2092603902 @default.
• W2051998700 cites W2112966757 @default.
• W2051998700 cites W2125429880 @default.
• W2051998700 cites W2132185105 @default.
• W2051998700 cites W2134449442 @default.
• W2051998700 cites W2141686420 @default.
• W2051998700 cites W2159744955 @default.
• W2051998700 cites W2216431321 @default.
• W2051998700 cites W2264018396 @default.
• W2051998700 cites W4205429959 @default.
• W2051998700 cites W9216576 @default.
• W2051998700 doi "https://doi.org/10.1016/s0169-328x(01)00021-3" @default.
• W2051998700 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11245923" @default.
• W2051998700 hasPublicationYear "2001" @default.
• W2051998700 type Work @default.
• W2051998700 sameAs 2051998700 @default.
• W2051998700 citedByCount "12" @default.
• W2051998700 countsByYear W20519987002012 @default.
• W2051998700 countsByYear W20519987002015 @default.
• W2051998700 countsByYear W20519987002017 @default.
• W2051998700 countsByYear W20519987002019 @default.
• W2051998700 countsByYear W20519987002020 @default.
• W2051998700 crossrefType "journal-article" @default.
• W2051998700 hasAuthorship W2051998700A5026360578 @default.
• W2051998700 hasAuthorship W2051998700A5035227436 @default.
• W2051998700 hasAuthorship W2051998700A5035243568 @default.
• W2051998700 hasAuthorship W2051998700A5048302807 @default.
• W2051998700 hasAuthorship W2051998700A5060429660 @default.
• W2051998700 hasAuthorship W2051998700A5060485274 @default.
• W2051998700 hasAuthorship W2051998700A5070846276 @default.
• W2051998700 hasConcept C101762097 @default.
• W2051998700 hasConcept C104317684 @default.
• W2051998700 hasConcept C121608353 @default.
• W2051998700 hasConcept C132376346 @default.
• W2051998700 hasConcept C134018914 @default.
• W2051998700 hasConcept C138885662 @default.
• W2051998700 hasConcept C150194340 @default.
• W2051998700 hasConcept C153911025 @default.
• W2051998700 hasConcept C167625842 @default.
• W2051998700 hasConcept C179926584 @default.
• W2051998700 hasConcept C21592294 @default.
• W2051998700 hasConcept C2778575703 @default.
• W2051998700 hasConcept C2778894405 @default.
• W2051998700 hasConcept C2781121885 @default.
• W2051998700 hasConcept C41895202 @default.
• W2051998700 hasConcept C51445715 @default.
• W2051998700 hasConcept C530470458 @default.
• W2051998700 hasConcept C54355233 @default.
• W2051998700 hasConcept C54985914 @default.
• W2051998700 hasConcept C71315377 @default.
• W2051998700 hasConcept C84606932 @default.
• W2051998700 hasConcept C86339819 @default.

• next