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- W2052032009 abstract "1. A thorough investigation of cultured fibroblastic cells (foetal and adult) for phenylalanine hydroxylase activity is being undertaken. Absence of this tissue-specific enzyme from cultured cells has been generally assumed, but no study of normal fibroblastic cells has been published. Another tissue-specific enzyme (cystathionine synthetase) has recently been found in adult cultured fibroblasts by other workers. 2. Foetal cells were studied first, and substantial amounts of activity were found in fibroblastic cells cultured from muscle, but not in fresh homogenates of foetal muscle. 3. Enzyme activity persisted throughout many subcultures, but showed marked daily variation within any one subculture. The cause of this variation has not yet been identified. 4. Studies of the basic defect in phenylketonuria will be aided greatly if phenylalanine hydroxylase activity can be demonstrated in cultured fibroblasts obtained post-natally. Preliminary studies of adult cells have been disappointing, but optimal conditions have probably not been achieved." @default.
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- W2052032009 date "1972-03-30" @default.
- W2052032009 modified "2023-09-25" @default.
- W2052032009 title "Phenylalanine hydroxylase activity in human foetal fibroblastic cells in culture" @default.
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- W2052032009 doi "https://doi.org/10.1016/0304-4165(72)90132-8" @default.
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