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• W205207834 abstract "Publisher Summary Both Heparin (HP) and heparan sulfate (HS) have a characteristic disaccharide repeat unit that comprises a uronic acid and a glucosamine residue. The uronic acid residue may be present as glucuronic acid or its C5 epimer, iduronic acid. HP and HS preparations are heterogeneous because of the high degree of complexity of the polysaccharide chain and the presence of many structural isomers. Conventional separation strategies fail to resolve constituent oligosaccharide pools to pure species alone. Usually, even with a combination of techniques, only a limited number of pure species may be obtained. To a great extent, everything depends on the ability to separate the oligosaccharide of interest at the outset and the subsequent oligosaccharides produced during the sequencing process. The separation of disaccharides derived from chemical degradation using nitrous acid cleavage poses more of a challenge. This cleavage occurs between the N -sulfated glucosamine residue and the hexuronic acid leaving a reducing end anhydromannose residue, which is usually reduced to anhydromannitol." @default.
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• W205207834 date "2005-01-01" @default.
• W205207834 modified "2023-09-23" @default.
• W205207834 title "Separation and Sequencing of Heparin and Heparan Sulphate Saccharides" @default.
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• W205207834 doi "https://doi.org/10.1016/b978-008044859-6/50007-1" @default.
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