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• W2052152201 startingPage "501" @default.
• W2052152201 abstract "Giardia duodenalis is a re-emerging protozoan parasite that causes diarrhoea in humans, significantly affecting the health of many people globally. To date, little is known about the genetic events underpinning the establishment of infection in host cells; however, the parasite's ventral disc, proteases and variable surface proteins (VSPs) are recognised as important pathogenic factors. In this study, representational difference analysis (RDA) was used to identify differentially expressed genes in four different Giardia isolates (WB, P-1, NF and GS/M) during the first 2h of in vitro interaction with the rat intestinal epithelial cell line, IEC-6. RDA showed that more than 40 genes were differentially expressed in each of the four Giardia isolates upon IEC-6 cells infection. Most of the up-regulated genes were common to the four isolates except for those encoding proteins possibly involved in immune evasion such as VSPs, high cysteine membrane proteins (HCMp), hypothetical proteins, and oxygen defence proteins (e.g., thioredoxin, peroxiredoxin 1). Differences in the expressed VSPs and HCMp may account for the variation in symptoms during giardiasis. Interestingly, the NF isolate solely expressed genes involved in encystation during interaction with IEC-6 (e.g., glucosamine 6-phosphate isomerase, dynamin, acid sphingomyelinase-like phosphodiesterase) suggesting that encystation signals could be different for this isolate. Common to the four isolates, transcripts for genes involved in glycolysis (e.g., glucose-6-phosphate dehydrogenase, fructose bisphosphate aldolase, enolase), attachment (γ and α1 giardins) and cysteine proteases were frequently detected. Genes involved in transcription, translation, signalling and cell cycle control were also up-regulated. This study shows that the RDA technique has selectively isolated genes involved in host-parasite interactions and complements previous microarray data. Some of the detected genes are also discussed as potential virulence factors and treatment targets in giardiasis." @default.
• W2052152201 created "2016-06-24" @default.
• W2052152201 creator A5006717488 @default.
• W2052152201 creator A5074233245 @default.
• W2052152201 date "2012-05-01" @default.
• W2052152201 modified "2023-09-25" @default.
• W2052152201 title "Representational difference analysis identifies specific genes in the interaction of Giardia duodenalis with the murine intestinal epithelial cell line, IEC-6" @default.
• W2052152201 cites W1527595659 @default.
• W2052152201 cites W1557046906 @default.
• W2052152201 cites W1564662492 @default.
• W2052152201 cites W1823081504 @default.
• W2052152201 cites W1875835053 @default.
• W2052152201 cites W1963770237 @default.
• W2052152201 cites W1964434157 @default.
• W2052152201 cites W1964472086 @default.
• W2052152201 cites W1970526067 @default.
• W2052152201 cites W1972059539 @default.
• W2052152201 cites W1975029578 @default.
• W2052152201 cites W1975336675 @default.
• W2052152201 cites W1981419554 @default.
• W2052152201 cites W1982506310 @default.
• W2052152201 cites W1983785927 @default.
• W2052152201 cites W1986401343 @default.
• W2052152201 cites W1989421641 @default.
• W2052152201 cites W1990154898 @default.
• W2052152201 cites W1992734990 @default.
• W2052152201 cites W1994301557 @default.
• W2052152201 cites W1996588353 @default.
• W2052152201 cites W1997192898 @default.
• W2052152201 cites W2000954320 @default.
• W2052152201 cites W2004216473 @default.
• W2052152201 cites W2009993911 @default.
• W2052152201 cites W2011991138 @default.
• W2052152201 cites W2015826097 @default.
• W2052152201 cites W2021372863 @default.
• W2052152201 cites W2022578820 @default.
• W2052152201 cites W2024400556 @default.
• W2052152201 cites W2027590165 @default.
• W2052152201 cites W2028378651 @default.
• W2052152201 cites W2030684171 @default.
• W2052152201 cites W2030712022 @default.
• W2052152201 cites W2032899389 @default.
• W2052152201 cites W2040864441 @default.
• W2052152201 cites W2043428681 @default.
• W2052152201 cites W2045875106 @default.
• W2052152201 cites W2047521162 @default.
• W2052152201 cites W2049108649 @default.
• W2052152201 cites W2053168795 @default.
• W2052152201 cites W2054156964 @default.
• W2052152201 cites W2055934359 @default.
• W2052152201 cites W2057100489 @default.
• W2052152201 cites W2061537983 @default.
• W2052152201 cites W2069972700 @default.
• W2052152201 cites W2072861322 @default.
• W2052152201 cites W2073178782 @default.
• W2052152201 cites W2077539793 @default.
• W2052152201 cites W2079690613 @default.
• W2052152201 cites W2082120087 @default.
• W2052152201 cites W2085224969 @default.
• W2052152201 cites W2088557128 @default.
• W2052152201 cites W2090612847 @default.
• W2052152201 cites W2092042635 @default.
• W2052152201 cites W2092771514 @default.
• W2052152201 cites W2092986690 @default.
• W2052152201 cites W2098974715 @default.
• W2052152201 cites W2101249834 @default.
• W2052152201 cites W2104371389 @default.
• W2052152201 cites W2105276311 @default.
• W2052152201 cites W2109494645 @default.
• W2052152201 cites W2113518661 @default.
• W2052152201 cites W2117159489 @default.
• W2052152201 cites W2117255228 @default.
• W2052152201 cites W2117628638 @default.
• W2052152201 cites W2117992130 @default.
• W2052152201 cites W2121090724 @default.
• W2052152201 cites W2126248785 @default.
• W2052152201 cites W2126942327 @default.
• W2052152201 cites W2127350765 @default.
• W2052152201 cites W2128928413 @default.
• W2052152201 cites W2133335149 @default.
• W2052152201 cites W2134571046 @default.
• W2052152201 cites W2135451596 @default.
• W2052152201 cites W2139617127 @default.
• W2052152201 cites W2141064935 @default.
• W2052152201 cites W2145702747 @default.
• W2052152201 cites W2149399366 @default.
• W2052152201 cites W2149489583 @default.
• W2052152201 cites W2152977880 @default.
• W2052152201 cites W2154607802 @default.
• W2052152201 cites W2158144866 @default.
• W2052152201 cites W2159886442 @default.
• W2052152201 cites W2164278290 @default.
• W2052152201 cites W2169999342 @default.
• W2052152201 cites W2170802619 @default.
• W2052152201 cites W2177561135 @default.
• W2052152201 cites W2331191252 @default.
• W2052152201 doi "https://doi.org/10.1016/j.ijpara.2012.04.004" @default.
• W2052152201 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22561399" @default.

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