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• W2052158752 abstract "Fluorimetrically determined pH-titration curves have been obtained for twelve purines and pyrimidines at room temperature, in aqueous solution. The pK(a) values for these compounds have been determined fluorimetrically and potentiometrically. Except for 6-mercaptopurine and uric acid, there is close agreement between the two sets of pK(a) values, but a large difference for the corresponding excited singlet-state values, pK(S1)(a), which were calculated previously. This indicates that the excited singlet-state proton-transfer rate is much slower than the fluorescence-decay rate of purines and pyrimidines in our experimental conditions. A direct, simple, pH-controlled fluorimetric method is proposed for the determination of purines and pyrimidines. The limits of detection vary between 60 ng ml and 5.4 mug ml ." @default.
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• W2052158752 date "1985-03-01" @default.
• W2052158752 modified "2023-09-26" @default.
• W2052158752 title "Fluorimetric determination of dissociation constants and ph-controlled fluorescence analysis of purines and pyrimidines" @default.
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• W2052158752 doi "https://doi.org/10.1016/0039-9140(85)80057-6" @default.
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