FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052163957> ?p ?o ?g. }

• W2052163957 endingPage "2538" @default.
• W2052163957 startingPage "2530" @default.
• W2052163957 abstract "Alphan alpha-amylase (TVA II) from Thermoactinomyces vulgaris R-47 efficiently hydrolyzes alpha-1,4-glucosidic linkages of pullulan to produce panose in addition to hydrolyzing starch. TVA II also hydrolyzes alpha-1,4-glucosidic linkages of cyclodextrins and alpha-1,6-glucosidic linkages of isopanose. To clarify the basis for this wide substrate specificity of TVA II, we soaked 4(3)-alpha-panosylpanose (4(3)-P2) (a pullulan hydrolysate composed of two panosyl units) into crystals of D325N inactive mutated TVA II. We then determined the crystal structure of TVA II complexed with 4(2)-alpha-panosylpanose (4(2)-P2), which was produced by transglycosylation from 4(3)-P2, at 2.2-A resolution. The shape of the active cleft of TVA II is unique among those of alpha-amylase family enzymes due to a loop (residues 193-218) that is located at the end of the cleft around the nonreducing region and forms a 'dam'-like bank. Because this loop is short in TVA II, the active cleft is wide and shallow around the nonreducing region. It is assumed that this short loop is one of the reasons for the wide substrate specificity of TVA II. While Trp356 is involved in the binding of Glc +2 of the substrate, it appears that Tyr374 in proximity to Trp356 plays two roles: one is fixing the orientation of Trp356 in the substrate-liganded state and the other is supplying the water that is necessary for substrate hydrolysis." @default.
• W2052163957 created "2016-06-24" @default.
• W2052163957 creator A5003653534 @default.
• W2052163957 creator A5009722297 @default.
• W2052163957 creator A5023503421 @default.
• W2052163957 creator A5043776203 @default.
• W2052163957 creator A5047791734 @default.
• W2052163957 creator A5066779869 @default.
• W2052163957 creator A5071132436 @default.
• W2052163957 creator A5085795182 @default.
• W2052163957 date "2004-06-01" @default.
• W2052163957 modified "2023-10-17" @default.
• W2052163957 title "The crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) complexed with transglycosylated product" @default.
• W2052163957 cites W1546896179 @default.
• W2052163957 cites W1772341111 @default.
• W2052163957 cites W1830745998 @default.
• W2052163957 cites W1935712031 @default.
• W2052163957 cites W1965251169 @default.
• W2052163957 cites W1967347076 @default.
• W2052163957 cites W1975824437 @default.
• W2052163957 cites W1976103643 @default.
• W2052163957 cites W1977709772 @default.
• W2052163957 cites W1984085600 @default.
• W2052163957 cites W1984910693 @default.
• W2052163957 cites W1993634515 @default.
• W2052163957 cites W1995017064 @default.
• W2052163957 cites W1996607909 @default.
• W2052163957 cites W2004904454 @default.
• W2052163957 cites W2010075079 @default.
• W2052163957 cites W2014231949 @default.
• W2052163957 cites W2016451409 @default.
• W2052163957 cites W2025928348 @default.
• W2052163957 cites W2028231353 @default.
• W2052163957 cites W2063191912 @default.
• W2052163957 cites W2066735672 @default.
• W2052163957 cites W2076805087 @default.
• W2052163957 cites W2078248419 @default.
• W2052163957 cites W2113964973 @default.
• W2052163957 cites W2120024227 @default.
• W2052163957 cites W2133552794 @default.
• W2052163957 cites W2147281477 @default.
• W2052163957 cites W2337892132 @default.
• W2052163957 cites W2888511473 @default.
• W2052163957 cites W4206285321 @default.
• W2052163957 cites W4231121696 @default.
• W2052163957 cites W4246566831 @default.
• W2052163957 doi "https://doi.org/10.1111/j.1432-1033.2004.04183.x" @default.
• W2052163957 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15182368" @default.
• W2052163957 hasPublicationYear "2004" @default.
• W2052163957 type Work @default.
• W2052163957 sameAs 2052163957 @default.
• W2052163957 citedByCount "21" @default.
• W2052163957 countsByYear W20521639572013 @default.
• W2052163957 countsByYear W20521639572015 @default.
• W2052163957 countsByYear W20521639572016 @default.
• W2052163957 countsByYear W20521639572017 @default.
• W2052163957 countsByYear W20521639572018 @default.
• W2052163957 countsByYear W20521639572019 @default.
• W2052163957 countsByYear W20521639572020 @default.
• W2052163957 countsByYear W20521639572022 @default.
• W2052163957 crossrefType "journal-article" @default.
• W2052163957 hasAuthorship W2052163957A5003653534 @default.
• W2052163957 hasAuthorship W2052163957A5009722297 @default.
• W2052163957 hasAuthorship W2052163957A5023503421 @default.
• W2052163957 hasAuthorship W2052163957A5043776203 @default.
• W2052163957 hasAuthorship W2052163957A5047791734 @default.
• W2052163957 hasAuthorship W2052163957A5066779869 @default.
• W2052163957 hasAuthorship W2052163957A5071132436 @default.
• W2052163957 hasAuthorship W2052163957A5085795182 @default.
• W2052163957 hasBestOaLocation W20521639571 @default.
• W2052163957 hasConcept C100817775 @default.
• W2052163957 hasConcept C170835558 @default.
• W2052163957 hasConcept C173959671 @default.
• W2052163957 hasConcept C181199279 @default.
• W2052163957 hasConcept C185592680 @default.
• W2052163957 hasConcept C18903297 @default.
• W2052163957 hasConcept C2777289219 @default.
• W2052163957 hasConcept C2779257994 @default.
• W2052163957 hasConcept C28800991 @default.
• W2052163957 hasConcept C41183919 @default.
• W2052163957 hasConcept C55493867 @default.
• W2052163957 hasConcept C71240020 @default.
• W2052163957 hasConcept C8010536 @default.
• W2052163957 hasConcept C86803240 @default.
• W2052163957 hasConcept C94412978 @default.
• W2052163957 hasConceptScore W2052163957C100817775 @default.
• W2052163957 hasConceptScore W2052163957C170835558 @default.
• W2052163957 hasConceptScore W2052163957C173959671 @default.
• W2052163957 hasConceptScore W2052163957C181199279 @default.
• W2052163957 hasConceptScore W2052163957C185592680 @default.
• W2052163957 hasConceptScore W2052163957C18903297 @default.
• W2052163957 hasConceptScore W2052163957C2777289219 @default.
• W2052163957 hasConceptScore W2052163957C2779257994 @default.
• W2052163957 hasConceptScore W2052163957C28800991 @default.
• W2052163957 hasConceptScore W2052163957C41183919 @default.
• W2052163957 hasConceptScore W2052163957C55493867 @default.
• W2052163957 hasConceptScore W2052163957C71240020 @default.
• W2052163957 hasConceptScore W2052163957C8010536 @default.

• next