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- W2052167124 abstract "Abstract The talc-Celite technique has now been adapted for the recovery of enteroviruses from potable waters. Layers containing a 3:1 mixture of talc and Celite 503 were sandwiched between two sheets of Whatman 114 filter paper with an AP 25 (Millipore) prefilter disc on top. For the processing of 1-1 volumes, layers (300 mg talc and 100 mg Celite) were held in a 47 mm diameter filter holder. Layers containing 1.2 g talc and 0.4 g Celite were prepared in 142 mm diameter metallic (Sartorius) holders for experiments with larger (10 to 1000 l) sample volumes. Before experimental contamination and passage through the layer, conditioning of dechlorinated water was carried out by adjusting its pH to 6.0 and the addition of Earle's balanced salt solution (EBSS) to a final concentration of 1:100. For 1-1 volumes, layer-adsorbed virus was eluted in situ with 10–20 ml of 10% fetal calf serum in normal saline (pH 9.0). Because 100 ml amounts of the same eluent were required when working with the larger layers, a 10-fold reduction in the volume of the eluate was achieved by placing it in a dialysis sac and subjecting it to overnight (4°C) hydroextraction with polyethylene glycol 6000. When working with poliovirus (type 1, Sabin)-contaminated sample volumes of 1- to 20-1, between 83–95% of the input virus could be recovered with this technique. In experiments with 100 to 1000 litre amounts, containing as little as 1.2 plaque forming units (PFU) of poliovirus 1 −1 , between 58–64% of the added virus could be recovered with a sample concentration of up to 100,000-fold. In 1- to 20-1 volume experiments with echovirus 6 and coxsackieviruses A9 and B5, 81–92% of the input PFU could be recovered." @default.
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- W2052167124 date "1979-01-01" @default.
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- W2052167124 title "Use of talc-celite layers in the concentration of enteroviruses from large volumes of potable waters" @default.
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- W2052167124 doi "https://doi.org/10.1016/0043-1354(79)90013-7" @default.
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