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• W2052173887 abstract "No AccessJournal of UrologyCLINICAL UROLOGY: Original Articles1 Mar 2002PROSPECTIVE EVALUATION OF GENETIC ABNORMALITIES AND TELOMERASE EXPRESSION IN EXFOLIATED URINARY CELLS FOR BLADDER CANCER DETECTION MANUEL NEVES, CALIN CIOFU, FRÉDÉRIQUE LAROUSSERIE, JOCELYNE FLEURY, MATHILDE SIBONY, ANTOINE FLAHAULT, FLORENT SOUBRIER, and BERNARD GATTEGNO MANUEL NEVESMANUEL NEVES More articles by this author , CALIN CIOFUCALIN CIOFU More articles by this author , FRÉDÉRIQUE LAROUSSERIEFRÉDÉRIQUE LAROUSSERIE More articles by this author , JOCELYNE FLEURYJOCELYNE FLEURY More articles by this author , MATHILDE SIBONYMATHILDE SIBONY More articles by this author , ANTOINE FLAHAULTANTOINE FLAHAULT More articles by this author , FLORENT SOUBRIERFLORENT SOUBRIER More articles by this author , and BERNARD GATTEGNOBERNARD GATTEGNO More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65281-0AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To evaluate alternative procedures to cytoscopic examination we prospectively compared noninvasive procedures for detecting bladder cancer namely cytology, loss of heterozygosity (LOH), microsatellite instability and human telomerase catalytic subunit reverse transcriptase (hTERT) messenger (m) RNA detection. Materials and Methods: Specificity and cutoff values were established in the blood and urine sediment of 50 controls. Sensitivity was analyzed in the urine and tissue samples of 50 patients with bladder cancer. The diagnosis was established by cystoscopic and histological examination. Genomic alterations were studied using a panel of 24 microsatellite markers to detect LOH events, while 3 additional mononucleotide repeats were analyzed for microsatellite instability detection. Telomerase expression was detected in urinary cells by nested RT-polymerase chain reaction amplification of hTERT mRNA. All techniques were compared by cytological examination. Results: Sensitivity and specificity were 31% and 100% for cytological testing, 96% and 100% for LOH, and 75% and 69% for RT-polymerase chain reaction of hTERT, respectively. No alteration was detected on microsatellite instability analysis in urine or tumor tissue cells. Using only the 5 markers most strongly associated with bladder cancer selected by logistic regression analysis, namely ABL1, IFNa, D9S12, MJD58 and D18S364, LOH test sensitivity slightly decreased to 90%. Conclusions: Urinary LOH analysis was the most sensitive and specific method for bladder cancer detection and it appeared less dependent on urine sediment quality. The logistic regression score may be an interesting complement to cystoscopy. The specificity of hTERT mRNA detection was incomplete since false-positives were observed in 31% of cases. Absent microsatellite instability in our cohort showed that these genomic alterations are not present at the early step of bladder cancer. References 1 : Le Cancer en France: Incidence et Mortalité, Situation en 1995. La Documentation Française. Paris: French Ministry of Health1998: 182. Google Scholar 2 : Superficial bladder cancer: progression and recurrence. J Urol1983; 130: 1083. 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Clin Cancer Res1998; 4: 2807. Google Scholar 11 : Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J1992; 11: 1921. Google Scholar 12 : Mismatch repair deficiency in phenotypically normal human cells. Science1995; 268: 738. Google Scholar 13 : Analysis of survival data: Cox and Weibull models with covariates. In: Statistics in Medical Research. Edited by . New York: Wiley1982: 365. Google Scholar 14 : Microsatellite instability in human solid tumors.. Cancer1998; 82: 1808. Google Scholar 15 : Four tumor suppressor loci on chromosome 9q in bladder cancer: evidence for two novel candidate regions at 9q22.3 and 9q31. Oncogene1999; 18: 157. Google Scholar 16 : Novel suppressor loci on chromosome 14q in primary bladder cancer. Cancer Res1995; 55: 3246. Google Scholar 17 : Loss of heterozygosity on chromosome 18q is associated with muscle- invasive transitional cell carcinoma of the bladder. W Br J Cancer1994; 70: 697. Google Scholar 18 : Frequency of homozygous deletion at p16/CDKN2 in primary human tumours. Nat Genet1995; 11: 210. Google Scholar 19 : Patterns of chromosomal imbalances in muscle invasive bladder cancer. Int J Oncol2000; 17: 1025. Google Scholar 20 : Comparison of telomerase activity in bladder carcinoma and exfoliated cells collected in urine and bladder washings, using a quantitative assay. Clin Cancer Res2000; 6: 2771. Google Scholar 21 : Limitations of urinary telomerase activity measurement in urothelial cancer. Clin Chim Acta2000; 296: 35. Google Scholar From the Laboratoire d’Histologie, Biologie Tumorale et Génétique Moléculaire, Service d’Urologie, Laboratoire d’Anatomie Pathologique, and Department d’Épidémiologie et de Biostatistique, Hôpital TENON, Paris, France© 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 167Issue 3March 2002Page: 1276-1281 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsmicrosatellite repeatsbladderbladderneoplasmstelomeraseloss of heterozygosityMetricsAuthor Information MANUEL NEVES More articles by this author CALIN CIOFU More articles by this author FRÉDÉRIQUE LAROUSSERIE More articles by this author JOCELYNE FLEURY More articles by this author MATHILDE SIBONY More articles by this author ANTOINE FLAHAULT More articles by this author FLORENT SOUBRIER More articles by this author BERNARD GATTEGNO More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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