FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052185611> ?p ?o ?g. }

• W2052185611 endingPage "704" @default.
• W2052185611 startingPage "693" @default.
• W2052185611 abstract "Background: Human embryonic stem cells (hESCs) are a potentially inexhaustible source of cells for replacement therapy. However, successful preclinical and clinical progress requires efficient and controlled differentiation towards the specific differentiated cell fate. Methods: We previously developed a strategy to generate blast cells (BCs) from hESCs that were capable of differentiating into vascular structures as well as into all hematopoietic cell lineages. Although the BCs were shown to repair damaged vasculature in multiple animal models, the large-scale generation of cells under these conditions was challenging. Here we report a simpler and more efficient method for robust generation of hemangioblastic progenitors. Results: In addition to eliminating several expensive factors that are unnecessary, we demonstrate that bone morphogenetic protein (BMP)-4 and VEGF are necessary and sufficient to induce hemangioblastic commitment and development from hESCs during early stages of differentiation. BMP-4 and VEGF significantly upregulate T-brachyury, KDR, CD31 and Lmo2 gene expression, while dramatically downregulating Oct-4 expression. The addition of basic FGF during growth and expansion was found to further enhance BC development, consistently generating approximately 1 × 10 8 BCs from one six well plate of hESCs. Conclusion: This new method represents a significantly improved system for generating hemangioblasts from hESCs, and although simplified, results in an eightfold increase in cell yield." @default.
• W2052185611 created "2016-06-24" @default.
• W2052185611 creator A5003550993 @default.
• W2052185611 creator A5006683878 @default.
• W2052185611 creator A5025464234 @default.
• W2052185611 creator A5046986404 @default.
• W2052185611 creator A5073612973 @default.
• W2052185611 creator A5091309173 @default.
• W2052185611 date "2008-09-01" @default.
• W2052185611 modified "2023-09-24" @default.
• W2052185611 title "Robust generation of hemangioblastic progenitors from human embryonic stem cells" @default.
• W2052185611 cites W1674015201 @default.
• W2052185611 cites W1977120253 @default.
• W2052185611 cites W1982960471 @default.
• W2052185611 cites W1992838550 @default.
• W2052185611 cites W2002768599 @default.
• W2052185611 cites W2006557127 @default.
• W2052185611 cites W2006562250 @default.
• W2052185611 cites W2030401873 @default.
• W2052185611 cites W2036314082 @default.
• W2052185611 cites W2036326354 @default.
• W2052185611 cites W2038404321 @default.
• W2052185611 cites W2038723730 @default.
• W2052185611 cites W2061997335 @default.
• W2052185611 cites W2064533525 @default.
• W2052185611 cites W2067817921 @default.
• W2052185611 cites W2069112909 @default.
• W2052185611 cites W2069781632 @default.
• W2052185611 cites W2070588786 @default.
• W2052185611 cites W2083763893 @default.
• W2052185611 cites W2085030176 @default.
• W2052185611 cites W2089078614 @default.
• W2052185611 cites W2098140873 @default.
• W2052185611 cites W2103696707 @default.
• W2052185611 cites W2107141327 @default.
• W2052185611 cites W2107277218 @default.
• W2052185611 cites W2110301828 @default.
• W2052185611 cites W2113416273 @default.
• W2052185611 cites W2120121992 @default.
• W2052185611 cites W2120297737 @default.
• W2052185611 cites W2163102268 @default.
• W2052185611 cites W2253665267 @default.
• W2052185611 cites W4242079987 @default.
• W2052185611 doi "https://doi.org/10.2217/17460751.3.5.693" @default.
• W2052185611 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18729794" @default.
• W2052185611 hasPublicationYear "2008" @default.
• W2052185611 type Work @default.
• W2052185611 sameAs 2052185611 @default.
• W2052185611 citedByCount "37" @default.
• W2052185611 countsByYear W20521856112012 @default.
• W2052185611 countsByYear W20521856112013 @default.
• W2052185611 countsByYear W20521856112014 @default.
• W2052185611 countsByYear W20521856112015 @default.
• W2052185611 countsByYear W20521856112016 @default.
• W2052185611 countsByYear W20521856112017 @default.
• W2052185611 countsByYear W20521856112018 @default.
• W2052185611 countsByYear W20521856112019 @default.
• W2052185611 countsByYear W20521856112020 @default.
• W2052185611 countsByYear W20521856112021 @default.
• W2052185611 crossrefType "journal-article" @default.
• W2052185611 hasAuthorship W2052185611A5003550993 @default.
• W2052185611 hasAuthorship W2052185611A5006683878 @default.
• W2052185611 hasAuthorship W2052185611A5025464234 @default.
• W2052185611 hasAuthorship W2052185611A5046986404 @default.
• W2052185611 hasAuthorship W2052185611A5073612973 @default.
• W2052185611 hasAuthorship W2052185611A5091309173 @default.
• W2052185611 hasConcept C104317684 @default.
• W2052185611 hasConcept C107459253 @default.
• W2052185611 hasConcept C145103041 @default.
• W2052185611 hasConcept C148738053 @default.
• W2052185611 hasConcept C201750760 @default.
• W2052185611 hasConcept C203014093 @default.
• W2052185611 hasConcept C2777117487 @default.
• W2052185611 hasConcept C2780915607 @default.
• W2052185611 hasConcept C28328180 @default.
• W2052185611 hasConcept C46122727 @default.
• W2052185611 hasConcept C54355233 @default.
• W2052185611 hasConcept C86803240 @default.
• W2052185611 hasConcept C95444343 @default.
• W2052185611 hasConcept C99854124 @default.
• W2052185611 hasConceptScore W2052185611C104317684 @default.
• W2052185611 hasConceptScore W2052185611C107459253 @default.
• W2052185611 hasConceptScore W2052185611C145103041 @default.
• W2052185611 hasConceptScore W2052185611C148738053 @default.
• W2052185611 hasConceptScore W2052185611C201750760 @default.
• W2052185611 hasConceptScore W2052185611C203014093 @default.
• W2052185611 hasConceptScore W2052185611C2777117487 @default.
• W2052185611 hasConceptScore W2052185611C2780915607 @default.
• W2052185611 hasConceptScore W2052185611C28328180 @default.
• W2052185611 hasConceptScore W2052185611C46122727 @default.
• W2052185611 hasConceptScore W2052185611C54355233 @default.
• W2052185611 hasConceptScore W2052185611C86803240 @default.
• W2052185611 hasConceptScore W2052185611C95444343 @default.
• W2052185611 hasConceptScore W2052185611C99854124 @default.
• W2052185611 hasIssue "5" @default.
• W2052185611 hasLocation W20521856111 @default.
• W2052185611 hasLocation W20521856112 @default.
• W2052185611 hasOpenAccess W2052185611 @default.

• next