Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052208096> ?p ?o ?g. }
- W2052208096 endingPage "104" @default.
- W2052208096 startingPage "95" @default.
- W2052208096 abstract "The human neuroblastoma cell line SH-SY5Y/TrkA differentiates in vitro and acquires a sympathetic phenotype in response to phorbolester (activator of protein kinase C, PKC) in the presence of serum or growth factors, or nerve growth factor (NGF). We have now investigated to what extent phorbolester and NGF cause activation of Ras and Raf-1 and the involvement of PKC in this response in differentiating SH-SY5Y/TrkA cells. NGF stimulated increased accumulation of Ras-GTP and a threefold activation of Raf-1. In contrast, 12-O-tetradecanoylphorbol-13-acetate (TPA) had no effect on the amount of Ras-GTP but led to a smaller activation of Raf-1. NGF caused a limited increase in phosphorylation of Raf-1 compared with TPA, and NGF-induced Raf activity was independent of PKC. Analysis of phosphorylation of the endogenous PKC substrate myristoylated alanine-rich C-kinase substrate (MARCKS), and of subcellular distribution of PKC-α, -δ, and -ε revealed that NGF only caused a very small activation of PKC in SH-SY5Y/TrkA cells. The results identify Raf-1 as a target for both TPA- and NGF-induced signals in differentiating SH-SY5Y/TrkA cells and demonstrate that signalling to Raf-1 was mediated via distinct mechanisms." @default.
- W2052208096 created "2016-06-24" @default.
- W2052208096 creator A5046599164 @default.
- W2052208096 creator A5063214114 @default.
- W2052208096 creator A5069818543 @default.
- W2052208096 creator A5072303267 @default.
- W2052208096 creator A5083737061 @default.
- W2052208096 date "2001-02-01" @default.
- W2052208096 modified "2023-09-24" @default.
- W2052208096 title "Activation of Ras, Raf-1 and protein kinase C in differentiating human neuroblastoma cells after treatment with phorbolester and NGF" @default.
- W2052208096 cites W1563745823 @default.
- W2052208096 cites W1572716768 @default.
- W2052208096 cites W1666849257 @default.
- W2052208096 cites W1675086682 @default.
- W2052208096 cites W1899641668 @default.
- W2052208096 cites W1969785844 @default.
- W2052208096 cites W1971727526 @default.
- W2052208096 cites W1975260251 @default.
- W2052208096 cites W1975702359 @default.
- W2052208096 cites W1978798502 @default.
- W2052208096 cites W1979582578 @default.
- W2052208096 cites W198944900 @default.
- W2052208096 cites W1989480480 @default.
- W2052208096 cites W1992660699 @default.
- W2052208096 cites W1993163893 @default.
- W2052208096 cites W1994541394 @default.
- W2052208096 cites W1995819749 @default.
- W2052208096 cites W1996962669 @default.
- W2052208096 cites W2001895687 @default.
- W2052208096 cites W2010296954 @default.
- W2052208096 cites W2010488494 @default.
- W2052208096 cites W2010955929 @default.
- W2052208096 cites W2019868249 @default.
- W2052208096 cites W2025870139 @default.
- W2052208096 cites W2026399166 @default.
- W2052208096 cites W2031452885 @default.
- W2052208096 cites W2037828896 @default.
- W2052208096 cites W2037831105 @default.
- W2052208096 cites W2040620685 @default.
- W2052208096 cites W2051354632 @default.
- W2052208096 cites W2079555764 @default.
- W2052208096 cites W2084098251 @default.
- W2052208096 cites W2091309613 @default.
- W2052208096 cites W2092232757 @default.
- W2052208096 cites W2100281377 @default.
- W2052208096 cites W2104102972 @default.
- W2052208096 cites W2109283410 @default.
- W2052208096 cites W2112533411 @default.
- W2052208096 cites W2116005813 @default.
- W2052208096 cites W2121619780 @default.
- W2052208096 cites W2136222819 @default.
- W2052208096 cites W2163421864 @default.
- W2052208096 cites W2316297264 @default.
- W2052208096 cites W297874522 @default.
- W2052208096 cites W74885494 @default.
- W2052208096 doi "https://doi.org/10.1016/s0898-6568(00)00141-8" @default.
- W2052208096 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11257453" @default.
- W2052208096 hasPublicationYear "2001" @default.
- W2052208096 type Work @default.
- W2052208096 sameAs 2052208096 @default.
- W2052208096 citedByCount "17" @default.
- W2052208096 countsByYear W20522080962013 @default.
- W2052208096 countsByYear W20522080962014 @default.
- W2052208096 countsByYear W20522080962015 @default.
- W2052208096 countsByYear W20522080962016 @default.
- W2052208096 countsByYear W20522080962021 @default.
- W2052208096 crossrefType "journal-article" @default.
- W2052208096 hasAuthorship W2052208096A5046599164 @default.
- W2052208096 hasAuthorship W2052208096A5063214114 @default.
- W2052208096 hasAuthorship W2052208096A5069818543 @default.
- W2052208096 hasAuthorship W2052208096A5072303267 @default.
- W2052208096 hasAuthorship W2052208096A5083737061 @default.
- W2052208096 hasConcept C11960822 @default.
- W2052208096 hasConcept C126322002 @default.
- W2052208096 hasConcept C134018914 @default.
- W2052208096 hasConcept C170493617 @default.
- W2052208096 hasConcept C171034665 @default.
- W2052208096 hasConcept C184235292 @default.
- W2052208096 hasConcept C185592680 @default.
- W2052208096 hasConcept C195794163 @default.
- W2052208096 hasConcept C2778423431 @default.
- W2052208096 hasConcept C2780780085 @default.
- W2052208096 hasConcept C502942594 @default.
- W2052208096 hasConcept C55493867 @default.
- W2052208096 hasConcept C71924100 @default.
- W2052208096 hasConcept C86803240 @default.
- W2052208096 hasConcept C88045685 @default.
- W2052208096 hasConcept C95444343 @default.
- W2052208096 hasConceptScore W2052208096C11960822 @default.
- W2052208096 hasConceptScore W2052208096C126322002 @default.
- W2052208096 hasConceptScore W2052208096C134018914 @default.
- W2052208096 hasConceptScore W2052208096C170493617 @default.
- W2052208096 hasConceptScore W2052208096C171034665 @default.
- W2052208096 hasConceptScore W2052208096C184235292 @default.
- W2052208096 hasConceptScore W2052208096C185592680 @default.
- W2052208096 hasConceptScore W2052208096C195794163 @default.
- W2052208096 hasConceptScore W2052208096C2778423431 @default.
- W2052208096 hasConceptScore W2052208096C2780780085 @default.