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- W2052209639 abstract "Adult golden perch, Macquaria ambigua, mature but do not ovulate and spawn in ponds. Ovulation and spawning were induced in the breeding season by injecting human chorionic gonadotrophin (HCG) and/or a preparation of the pituitary gland from common carp (CPG). Spawning was induced in all females injected with dosages of 500, 1000 and 2000 IU/kg HCG or 5, 10 and 15 mg/kg CPG. Dosages of 100 and 200 IU/kg HCG or 2 mg/kg CPG induced ovulation in most females but only the more mature fish spawned. Dosages of 50 IU/kg HCG and 1 mg/kg CPG induced oocyte maturation but not ovulation. Males, injected with 200 IU/kg HCG plus 2 or 5 mg/kg CPG, were placed with one or two females in 2000-l concrete tanks, and spawning occurred 26–38 h after injection at a water temperature of 25°C. The injection of females with sub-threshold spawning dosages resulted in latency periods to spawning which were significantly longer than those after the injection of threshold or above-threshold spawning dosages. The fertilization rates were generally higher than 80%. The highest mean hatch rate of 87.4% was achieved using a dosage of 500 IU/kg HCG, and the largest dosage, 2000 IU/kg HCG, resulted in a significantly lower mean hatch rate of 44.2%. The significantly greater variation of hatch rates of eggs spawned by females injected with 5 mg/kg CPG by comparison with the variation of hatch rates after injection with 500 IU/kg HCG, and the different effects that a doubling of these spawning threshold dosages had on the hatch rates, suggested there were inconsistent levels of gonadotrophin in the crude pituitary preparations. HCG is recommended for the hormone-induced spawning of M. ambigua. Techniques for broodstock selection, injection, spawning and egg incubation are described." @default.
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- W2052209639 date "1983-01-01" @default.
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- W2052209639 title "The hormone-induced ovulation and spawning of the Australian freshwater fish golden perch, Macquaria ambigua (Richardson) (Percichthyidae)" @default.
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- W2052209639 doi "https://doi.org/10.1016/0044-8486(83)90093-5" @default.
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