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• W2052239024 abstract "Endogenous nitric oxide (NO) appears to modulate many physiological and pathophysiological processes. In order to obtain direct evidence for NO functions in vivo, we have developed 4,5-diaminofluorescein (DAF-2) as a novel fluorescent indicator for NO. Green-fluorescent triazolofluorescein formed by the reaction of NO and DAF-2 affords high sensitivity for NO (detection limit: 5 nM). Membrane-permeable DAF-2 diacetate (DAF-2 DA) was loaded into activated rat aortic smooth muscle cells, where the ester bonds are hydrolyzed by intracellular esterase, generating DAF-2. The fluorescence in the cells increased in a NO concentration-dependent manner. This imaging method should be useful for studies of the dynamic biological actions of NO at the molecular level with fine temporal and spatial resolution." @default.
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• W2052239024 date "1998-01-01" @default.
• W2052239024 modified "2023-10-15" @default.
• W2052239024 title "Development of a Fluorescent Indicator for Nitric Oxide Based on the Fluorescein Chromophore." @default.
• W2052239024 doi "https://doi.org/10.1248/cpb.46.373" @default.
• W2052239024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9501473" @default.
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