Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052256157> ?p ?o ?g. }
- W2052256157 endingPage "419" @default.
- W2052256157 startingPage "407" @default.
- W2052256157 abstract "A reversed-phase high-performance liquid chromatographic method for the separation of human haemoglobin chains has been devised. Using a LiChrospher 100 CH-8/2 column and a ternary eluent (acetonitrile-methanol-0.155 M NaCl, pH 2.7) improved resolution was achieved between (δβ) Lepore, βA, βS, α, Gγ and Aγ chains within a 60-min linear gradient. The AγT chain can also be separated by increasing the gradient time and decreasing the flow-rate. Silanophilic interactions play an important role in the retention mechanism, and NACl addition was necessary in order to suppress adsorption on free silanols. Increasing the methanol concentration to 10% caused a slight increase in chain retention, probably owing to solvation of the stationary phase. The recovery was 82% and the reproducibility of retention times was as good as −1.5%. Quantitation of chains is likely to be possible by peak area measurement. Owing to its sensitivity, the proposed method may be useful in the diagnosis of haemoglobinopathies and in the study of haemoglobin variants." @default.
- W2052256157 created "2016-06-24" @default.
- W2052256157 creator A5015231246 @default.
- W2052256157 creator A5029104371 @default.
- W2052256157 creator A5041156295 @default.
- W2052256157 creator A5053761369 @default.
- W2052256157 date "1985-01-01" @default.
- W2052256157 modified "2023-09-23" @default.
- W2052256157 title "Reversed-phase high-performance liquid chromatography of human haemoglobin chains" @default.
- W2052256157 cites W1547680166 @default.
- W2052256157 cites W1589225197 @default.
- W2052256157 cites W1607360837 @default.
- W2052256157 cites W16596340 @default.
- W2052256157 cites W1970775870 @default.
- W2052256157 cites W1976187067 @default.
- W2052256157 cites W1982225410 @default.
- W2052256157 cites W1985655173 @default.
- W2052256157 cites W1993625264 @default.
- W2052256157 cites W1995797614 @default.
- W2052256157 cites W2003412983 @default.
- W2052256157 cites W2009789225 @default.
- W2052256157 cites W2023758525 @default.
- W2052256157 cites W2030587756 @default.
- W2052256157 cites W2033719181 @default.
- W2052256157 cites W2037785662 @default.
- W2052256157 cites W2037830205 @default.
- W2052256157 cites W2041070122 @default.
- W2052256157 cites W2045484922 @default.
- W2052256157 cites W2050346937 @default.
- W2052256157 cites W2056718050 @default.
- W2052256157 cites W2060579461 @default.
- W2052256157 cites W2071147711 @default.
- W2052256157 cites W2072251512 @default.
- W2052256157 cites W2075180152 @default.
- W2052256157 cites W2075877548 @default.
- W2052256157 cites W2079796161 @default.
- W2052256157 cites W2085051706 @default.
- W2052256157 cites W2088027353 @default.
- W2052256157 cites W2088852772 @default.
- W2052256157 cites W2093150055 @default.
- W2052256157 cites W2126074191 @default.
- W2052256157 cites W2341515519 @default.
- W2052256157 cites W2397057473 @default.
- W2052256157 cites W2414401349 @default.
- W2052256157 cites W2421413352 @default.
- W2052256157 cites W4246427016 @default.
- W2052256157 doi "https://doi.org/10.1016/s0021-9673(01)90459-5" @default.
- W2052256157 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3988844" @default.
- W2052256157 hasPublicationYear "1985" @default.
- W2052256157 type Work @default.
- W2052256157 sameAs 2052256157 @default.
- W2052256157 citedByCount "71" @default.
- W2052256157 countsByYear W20522561572012 @default.
- W2052256157 countsByYear W20522561572013 @default.
- W2052256157 countsByYear W20522561572014 @default.
- W2052256157 countsByYear W20522561572015 @default.
- W2052256157 countsByYear W20522561572016 @default.
- W2052256157 countsByYear W20522561572017 @default.
- W2052256157 countsByYear W20522561572020 @default.
- W2052256157 countsByYear W20522561572023 @default.
- W2052256157 crossrefType "journal-article" @default.
- W2052256157 hasAuthorship W2052256157A5015231246 @default.
- W2052256157 hasAuthorship W2052256157A5029104371 @default.
- W2052256157 hasAuthorship W2052256157A5041156295 @default.
- W2052256157 hasAuthorship W2052256157A5053761369 @default.
- W2052256157 hasConcept C113196181 @default.
- W2052256157 hasConcept C121332964 @default.
- W2052256157 hasConcept C138268822 @default.
- W2052256157 hasConcept C148093993 @default.
- W2052256157 hasConcept C150394285 @default.
- W2052256157 hasConcept C154945302 @default.
- W2052256157 hasConcept C172120300 @default.
- W2052256157 hasConcept C178790620 @default.
- W2052256157 hasConcept C179998833 @default.
- W2052256157 hasConcept C185592680 @default.
- W2052256157 hasConcept C199360897 @default.
- W2052256157 hasConcept C2777463227 @default.
- W2052256157 hasConcept C2779607525 @default.
- W2052256157 hasConcept C2780471494 @default.
- W2052256157 hasConcept C3020018676 @default.
- W2052256157 hasConcept C41008148 @default.
- W2052256157 hasConcept C43617362 @default.
- W2052256157 hasConcept C44280652 @default.
- W2052256157 hasConcept C45188465 @default.
- W2052256157 hasConcept C51724486 @default.
- W2052256157 hasConcept C62520636 @default.
- W2052256157 hasConcept C64452783 @default.
- W2052256157 hasConcept C9893847 @default.
- W2052256157 hasConceptScore W2052256157C113196181 @default.
- W2052256157 hasConceptScore W2052256157C121332964 @default.
- W2052256157 hasConceptScore W2052256157C138268822 @default.
- W2052256157 hasConceptScore W2052256157C148093993 @default.
- W2052256157 hasConceptScore W2052256157C150394285 @default.
- W2052256157 hasConceptScore W2052256157C154945302 @default.
- W2052256157 hasConceptScore W2052256157C172120300 @default.
- W2052256157 hasConceptScore W2052256157C178790620 @default.
- W2052256157 hasConceptScore W2052256157C179998833 @default.
- W2052256157 hasConceptScore W2052256157C185592680 @default.