Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052294328> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W2052294328 endingPage "388" @default.
- W2052294328 startingPage "378" @default.
- W2052294328 abstract "EcoP15I DNA methyltransferase (Mtase) recognizes the asymmeteric sequence CAGCAG and catalyzes the transfer of a methyl group from S -adenosyl- l -methionine to the second adenine residue. We have investigated the DNA binding properties of EcoP15I DNA Mtase using gel mobility shift assays. EcoP15I DNA Mtase binds approximately threefold more tightly to DNA containing its recognition sequence, CAGCAG, than to non-specific sequences in the absence or presence of cofactors. Interestingly, in the presence of ATP the discrimination between specific and non-specific sequences increases significantly. These results suggest for the first time a role for ATP in DNA recognition by type III restriction-modification enzymes. In addition, we have shown that bromodeoxyuridine-containing oligonucleotides form complexes with EcoP15I DNA Mtase that are crosslinked upon irradiation. More importantly, we have shown that the crosslink site is at the site of DNA binding, since it can be suppressed by an excess of unmodified oligonucleotide. EcoP15I DNA Mtase exhibited Michaelis-Menten kinetics with both unmodified and bromodeoxyuridine-substituted DNA, with a higher specificity constant for the latter. Furthermore, gel mobility shift assays showed that proteolyzed EcoP15I DNA Mtase formed a specific complex with DNA, which had similar mobility as the native protein-DNA complex. Taken together these results form the basis for a detailed structure-function analysis of EcoP15I DNA Mtase." @default.
- W2052294328 created "2016-06-24" @default.
- W2052294328 creator A5021599987 @default.
- W2052294328 creator A5041421289 @default.
- W2052294328 date "1994-09-01" @default.
- W2052294328 modified "2023-09-26" @default.
- W2052294328 title "Interaction of EcoP15I DNA Methyltransferase with Oligonucleotides Containing the Asymmetric Sequence 5′-CAGCAG-3′" @default.
- W2052294328 doi "https://doi.org/10.1006/jmbi.1994.1588" @default.
- W2052294328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7932697" @default.
- W2052294328 hasPublicationYear "1994" @default.
- W2052294328 type Work @default.
- W2052294328 sameAs 2052294328 @default.
- W2052294328 citedByCount "37" @default.
- W2052294328 countsByYear W20522943282012 @default.
- W2052294328 countsByYear W20522943282016 @default.
- W2052294328 crossrefType "journal-article" @default.
- W2052294328 hasAuthorship W2052294328A5021599987 @default.
- W2052294328 hasAuthorship W2052294328A5041421289 @default.
- W2052294328 hasConcept C129312508 @default.
- W2052294328 hasConcept C153911025 @default.
- W2052294328 hasConcept C185592680 @default.
- W2052294328 hasConcept C2779979206 @default.
- W2052294328 hasConcept C33288867 @default.
- W2052294328 hasConcept C51679486 @default.
- W2052294328 hasConcept C552990157 @default.
- W2052294328 hasConcept C55493867 @default.
- W2052294328 hasConcept C86803240 @default.
- W2052294328 hasConcept C91965660 @default.
- W2052294328 hasConceptScore W2052294328C129312508 @default.
- W2052294328 hasConceptScore W2052294328C153911025 @default.
- W2052294328 hasConceptScore W2052294328C185592680 @default.
- W2052294328 hasConceptScore W2052294328C2779979206 @default.
- W2052294328 hasConceptScore W2052294328C33288867 @default.
- W2052294328 hasConceptScore W2052294328C51679486 @default.
- W2052294328 hasConceptScore W2052294328C552990157 @default.
- W2052294328 hasConceptScore W2052294328C55493867 @default.
- W2052294328 hasConceptScore W2052294328C86803240 @default.
- W2052294328 hasConceptScore W2052294328C91965660 @default.
- W2052294328 hasIssue "4" @default.
- W2052294328 hasLocation W20522943281 @default.
- W2052294328 hasLocation W20522943282 @default.
- W2052294328 hasOpenAccess W2052294328 @default.
- W2052294328 hasPrimaryLocation W20522943281 @default.
- W2052294328 hasRelatedWork W1980265640 @default.
- W2052294328 hasRelatedWork W2014038042 @default.
- W2052294328 hasRelatedWork W2017191738 @default.
- W2052294328 hasRelatedWork W2052294328 @default.
- W2052294328 hasRelatedWork W2059346522 @default.
- W2052294328 hasRelatedWork W2059862594 @default.
- W2052294328 hasRelatedWork W2143007402 @default.
- W2052294328 hasRelatedWork W2159813321 @default.
- W2052294328 hasRelatedWork W2319204232 @default.
- W2052294328 hasRelatedWork W2897587918 @default.
- W2052294328 hasVolume "242" @default.
- W2052294328 isParatext "false" @default.
- W2052294328 isRetracted "false" @default.
- W2052294328 magId "2052294328" @default.
- W2052294328 workType "article" @default.