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- W2052428534 abstract "A double antibody enzyme immunoassay of plasma cortisol was established using β-galactosidase from E. coli as the tracer. Cortisol-21-hemisuccinate was conjugated with β-galactosidase using a water-soluble carbodiimide. An antibody raised in the rabbit against cortisol-21-hemisuccinate-bovine serum albumin was used as the first antibody and anti-rabbit γ-globulin goat serum was used as the second. The sensitivity of the present enzyme immunoassay was 25 pg/tube. The method satisfied general reliability criteria regarding specificity, accuracy and precision. Plasma cortisol levels estimated by the enzyme immunoassay was highly correlated (r = 0.99, P < 0.005) with the levels measured by radioimmunoassay. This method of enzyme immunoassay was found to be of practical application to the routine assay of plasma cortisol." @default.
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- W2052428534 date "1981-07-01" @default.
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- W2052428534 title "Double antibody enzyme immunoassay of cortisol in bovine plasma" @default.
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- W2052428534 doi "https://doi.org/10.1016/0039-128x(81)90024-6" @default.
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