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- W2052476500 abstract "ABSTRACT An antiserum to chorionic gonadotrophin (HCG) has been studied in agar diffusion, haemagglutination and haemagglutination inhibition experiments. The anti-HCG serum contained antibodies to inert urinary proteins and serum proteins as well as a »hormone-specific« antibody. Absorption of the anti-HCG serum with small amounts of inert urinary protein removed precipitins to non-hormonal protein, without altering the haemagglutinating titre of the antiserum. There was no difference between absorbed and unabsorbed anti-HCG serum in haemagglutination inhibition reactions with inert and gonadotrophic urinary extracts. When larger amounts of inert protein were used for absorption there was a fall in the haemagglutinating titre of the anti-serum but it was shown that this was due to removal of »hormone specific« antibodies. Urinary extracts were obtained from patients with various endocrine disorders, from patients with normal pituitary and gonadal function and from menopausal subjects. The results of bioassay and immunoassay of these extracts were compared. It was found that many of the extracts, although potent in the immunoassay, were biologically inactive. It is concluded that a specific or non-specific reaction can occur between nonhormonal urinary proteins and the antihormone. The anti-HCG serum used in the present investigation, although successfully used for pregnancy diagnosis and HCG assay, was unsuitable for urinary gonadotrophin estimation in non-pregnant individuals." @default.
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- W2052476500 date "1965-10-01" @default.
- W2052476500 modified "2023-09-24" @default.
- W2052476500 title "A STUDY OF THE IMMUNOLOGICAL DETERMINATION OF PITUITARY GONADOTROPHIN IN URINE" @default.
- W2052476500 doi "https://doi.org/10.1530/acta.0.0500224" @default.
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