FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052481750> ?p ?o ?g. }

• W2052481750 endingPage "200" @default.
• W2052481750 startingPage "194" @default.
• W2052481750 abstract "ABSTRACT We used site-directed mutagenesis to probe the function of four alternating arginines located at amino acid positions 525, 527, 529, and 531 in a highly conserved region of domain III in the Cry1Ac toxin of Bacillus thuringiensis . We created 10 mutants: eight single mutants, with each arginine replaced by either glycine (G) or aspartic acid (D), and two double mutants (R525G/R527G and R529G/R531G). In lawn assays of the 10 mutants with a cultured Choristoneura fumiferana insect cell line (Cf1), replacement of a single arginine by either glycine or aspartic acid at position 525 or 529 decreased toxicity 4- to 12-fold relative to native Cry1Ac toxin, whereas replacement at position 527 or 531 decreased toxicity only 3-fold. The reduction in toxicity seen with double mutants was 8-fold for R525G/R527G and 25-fold for R529G/R531G. Five of the mutants (R525G, R525D, R527G, R529D, and R525G/R527G) were tested in bioassays with Plutella xylostella larvae and ion channel formation in planar lipid bilayers. In the bioassays, R525D, R529D, and R525G/R527G showed reduced toxicity. In planar lipid bilayers, the conductance and the selectivity of the mutants were similar to those of native Cry1Ac. Toxins with alteration at position 527 or 529 tended to remain in their subconducting states rather than the maximally conducting state. Our results suggest that the primary role of this conserved region is to maintain both the structural integrity of the native toxin and the full functionality of the formed membrane pore." @default.
• W2052481750 created "2016-06-24" @default.
• W2052481750 creator A5001267710 @default.
• W2052481750 creator A5001454091 @default.
• W2052481750 creator A5002290433 @default.
• W2052481750 creator A5021207885 @default.
• W2052481750 creator A5027148796 @default.
• W2052481750 creator A5080072204 @default.
• W2052481750 creator A5086479845 @default.
• W2052481750 date "2002-01-01" @default.
• W2052481750 modified "2023-10-17" @default.
• W2052481750 title "Mutagenic Analysis of a Conserved Region of Domain III in the Cry1Ac Toxin of <i>Bacillus thuringiensis</i>" @default.
• W2052481750 cites W1534255306 @default.
• W2052481750 cites W1537644103 @default.
• W2052481750 cites W1752586708 @default.
• W2052481750 cites W1765689133 @default.
• W2052481750 cites W1977448820 @default.
• W2052481750 cites W1986057351 @default.
• W2052481750 cites W1986912692 @default.
• W2052481750 cites W2006086298 @default.
• W2052481750 cites W2010672864 @default.
• W2052481750 cites W2014670968 @default.
• W2052481750 cites W2031388815 @default.
• W2052481750 cites W2035077342 @default.
• W2052481750 cites W2043993990 @default.
• W2052481750 cites W2048210780 @default.
• W2052481750 cites W2057313862 @default.
• W2052481750 cites W2070207092 @default.
• W2052481750 cites W2073427943 @default.
• W2052481750 cites W2078675196 @default.
• W2052481750 cites W2081335753 @default.
• W2052481750 cites W2095217745 @default.
• W2052481750 cites W2107847732 @default.
• W2052481750 cites W2114417464 @default.
• W2052481750 cites W2131612651 @default.
• W2052481750 cites W2133956507 @default.
• W2052481750 cites W2140829471 @default.
• W2052481750 cites W2156114416 @default.
• W2052481750 cites W2170577949 @default.
• W2052481750 cites W4293247451 @default.
• W2052481750 doi "https://doi.org/10.1128/aem.68.1.194-200.2002" @default.
• W2052481750 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/126535" @default.
• W2052481750 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11772627" @default.
• W2052481750 hasPublicationYear "2002" @default.
• W2052481750 type Work @default.
• W2052481750 sameAs 2052481750 @default.
• W2052481750 citedByCount "50" @default.
• W2052481750 countsByYear W20524817502012 @default.
• W2052481750 countsByYear W20524817502013 @default.
• W2052481750 countsByYear W20524817502014 @default.
• W2052481750 countsByYear W20524817502015 @default.
• W2052481750 countsByYear W20524817502016 @default.
• W2052481750 countsByYear W20524817502018 @default.
• W2052481750 countsByYear W20524817502019 @default.
• W2052481750 countsByYear W20524817502021 @default.
• W2052481750 countsByYear W20524817502022 @default.
• W2052481750 crossrefType "journal-article" @default.
• W2052481750 hasAuthorship W2052481750A5001267710 @default.
• W2052481750 hasAuthorship W2052481750A5001454091 @default.
• W2052481750 hasAuthorship W2052481750A5002290433 @default.
• W2052481750 hasAuthorship W2052481750A5021207885 @default.
• W2052481750 hasAuthorship W2052481750A5027148796 @default.
• W2052481750 hasAuthorship W2052481750A5080072204 @default.
• W2052481750 hasAuthorship W2052481750A5086479845 @default.
• W2052481750 hasBestOaLocation W20524817501 @default.
• W2052481750 hasConcept C102230213 @default.
• W2052481750 hasConcept C103408237 @default.
• W2052481750 hasConcept C104317684 @default.
• W2052481750 hasConcept C104488531 @default.
• W2052481750 hasConcept C122172344 @default.
• W2052481750 hasConcept C143065580 @default.
• W2052481750 hasConcept C16318435 @default.
• W2052481750 hasConcept C181199279 @default.
• W2052481750 hasConcept C2775880066 @default.
• W2052481750 hasConcept C2776414213 @default.
• W2052481750 hasConcept C2777272437 @default.
• W2052481750 hasConcept C2777367657 @default.
• W2052481750 hasConcept C2777468819 @default.
• W2052481750 hasConcept C2777932965 @default.
• W2052481750 hasConcept C2779035793 @default.
• W2052481750 hasConcept C2781151318 @default.
• W2052481750 hasConcept C2781208047 @default.
• W2052481750 hasConcept C515207424 @default.
• W2052481750 hasConcept C523546767 @default.
• W2052481750 hasConcept C54355233 @default.
• W2052481750 hasConcept C55493867 @default.
• W2052481750 hasConcept C86803240 @default.
• W2052481750 hasConceptScore W2052481750C102230213 @default.
• W2052481750 hasConceptScore W2052481750C103408237 @default.
• W2052481750 hasConceptScore W2052481750C104317684 @default.
• W2052481750 hasConceptScore W2052481750C104488531 @default.
• W2052481750 hasConceptScore W2052481750C122172344 @default.
• W2052481750 hasConceptScore W2052481750C143065580 @default.
• W2052481750 hasConceptScore W2052481750C16318435 @default.
• W2052481750 hasConceptScore W2052481750C181199279 @default.
• W2052481750 hasConceptScore W2052481750C2775880066 @default.
• W2052481750 hasConceptScore W2052481750C2776414213 @default.
• W2052481750 hasConceptScore W2052481750C2777272437 @default.

• next