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- W2052484927 abstract "In vitro models of osteogenesis are essential for investigating bone biology and the effects of pharmaceutical, chemical, and physical cues on bone formation. Osteogenesis takes place in a complex three-dimensional (3D) environment with cells from both mesenchymal and hematopoietic origins. Existing in vitro models of osteogenesis include two-dimensional (2D) single type cell monolayers and 3D cultures. However, an in vitro scaffold-free multicellular 3D model of osteogenesis is missing. We hypothesized that the self-inductive ossification capacity of bone marrow tissue can be harnessed in vitro and employed as a scaffold-free multicellular 3D model of osteogenesis. Therefore, rat bone marrow tissue was cultured for 28 days in three settings: 2D monolayer, 3D homogenized pellet, and 3D organotypic explant. The ossification potential of marrow in each condition was quantified by micro-computed tomography. The 3D organotypic marrow explant culture resulted in the greatest level of ossification with plate-like bone formations (up to 5 mm in diameter and 0.24 mm in thickness). To evaluate the mimicry of the organotypic marrow explants to newly forming native bone tissue, detailed compositional and morphological analyses were performed, including characterization of the ossified matrix by histochemistry, immunohistochemistry, Raman microspectroscopy, energy dispersive X-ray spectroscopy, backscattered electron microscopy, and micromechanical tests. The results indicated that the 3D organotypic marrow explant culture model mimics newly forming native bone tissue in terms of the characteristics studied. Therefore, this platform holds significant potential to be used as a model of osteogenesis, offering an alternative to in vitro monolayer cultures and in vivo animal models." @default.
- W2052484927 created "2016-06-24" @default.
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- W2052484927 creator A5078624568 @default.
- W2052484927 date "2011-02-12" @default.
- W2052484927 modified "2023-09-26" @default.
- W2052484927 title "A Scaffold-Free Multicellular Three-Dimensional In Vitro Model of Osteogenesis" @default.
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- W2052484927 doi "https://doi.org/10.1007/s00223-011-9467-3" @default.
- W2052484927 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21318400" @default.
- W2052484927 hasPublicationYear "2011" @default.
- W2052484927 type Work @default.