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- W2052538813 abstract "We have studied the fundamentals of gene expression in the protozoan parasite Toxoplasma gondii by analyzing, in detail, the genes encoding α- and β-tubulin. Southern analysis and quantitation studies reveal that, unlike in other organisms studied thus far, both these genes are present as single copies in the haploid Toxoplasma genome. Sequencing of these genes indicates that they both contain multiple introns with conserved 5′ and 3′ splice site signals. We have found that HeLa cell nuclear extracts are able to accurately splice a Toxoplasma pre-mRNA construct. We have mapped, for the α-tubulin gene, the exact site of transcription initiation and the approximate site of poly A addition by primer extension and RNase protection assays. Trans-splicing, as demonstrated in the Kinetoplastida, is not involved in the formation of the mature α-tubulin transcript in T. gondii." @default.
- W2052538813 created "2016-06-24" @default.
- W2052538813 creator A5053925806 @default.
- W2052538813 creator A5086159639 @default.
- W2052538813 date "1988-06-01" @default.
- W2052538813 modified "2023-09-27" @default.
- W2052538813 title "The α- and β-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple introns" @default.
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- W2052538813 doi "https://doi.org/10.1016/0166-6851(88)90081-3" @default.
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