FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052544105> ?p ?o ?g. }

• W2052544105 endingPage "1127" @default.
• W2052544105 startingPage "1119" @default.
• W2052544105 abstract "Background Recognition of donor antigens can occur through two separate pathways: the direct pathway (non-self HLA on donor cells) and the indirect pathway (self-restricted presentation of donor derived peptides on recipient cells). Indirect allorecognition is important in the development of humoral rejection; therefore, there is an increasing interest in the monitoring of indirect alloreactive T-cells. We have used an in vitro model to determine the optimal requirements for indirect presentation and assessed the risk for semidirect presentation in this system. Methods HLA-typed monocyte-derived dendritic cells (moDCs) were incubated with cellular fragments or necrotic cells and incubated with either indirect or direct alloreactive T-cell clones. T-cell reactivity was measured through proliferation or cytokine secretion. HLA-typed moDC, monocytes, or PBMCs were incubated with HLA class I monomers, in combination with either direct/indirect T-cell clones. Results Although both were efficiently taken up, alloreactivity was limited to the semi-direct pathway, as measured by allospecific CD4 (indirect) and CD8 T-cell clones (direct) when cells were used. In contrast, HLA-A2 monomers were not only efficiently taken up but also processed and presented by HLA-typed moDC, monocytes, and PBMCs. Activation was shown by a dose-dependent induction of IFN-γ production and proliferation by the CD4 T-cell clone. Antigen presentation was most efficient when the monomers were cultured for longer periods (24–48 hr) in the presence of the T-cells. Using this method, no reactivity was observed by the CD8 T-cell clone, confirming no semidirect alloreactivity. Conclusion We have developed a system that could be used to monitor indirect alloreactive T-cells." @default.
• W2052544105 created "2016-06-24" @default.
• W2052544105 creator A5019013671 @default.
• W2052544105 creator A5021544227 @default.
• W2052544105 creator A5031428632 @default.
• W2052544105 creator A5042295492 @default.
• W2052544105 creator A5045144667 @default.
• W2052544105 creator A5056617953 @default.
• W2052544105 creator A5058862472 @default.
• W2052544105 creator A5069641539 @default.
• W2052544105 date "2014-06-15" @default.
• W2052544105 modified "2023-10-03" @default.
• W2052544105 title "HLA Monomers as a Tool to Monitor Indirect Allorecognition" @default.
• W2052544105 cites W1496266479 @default.
• W2052544105 cites W1505062935 @default.
• W2052544105 cites W1535969196 @default.
• W2052544105 cites W1570731639 @default.
• W2052544105 cites W1666706706 @default.
• W2052544105 cites W1669165205 @default.
• W2052544105 cites W1968797624 @default.
• W2052544105 cites W1969347248 @default.
• W2052544105 cites W1974039913 @default.
• W2052544105 cites W1975447679 @default.
• W2052544105 cites W1980211566 @default.
• W2052544105 cites W1987810275 @default.
• W2052544105 cites W1990029812 @default.
• W2052544105 cites W1994121614 @default.
• W2052544105 cites W1995716593 @default.
• W2052544105 cites W1997244864 @default.
• W2052544105 cites W2008002517 @default.
• W2052544105 cites W2018604730 @default.
• W2052544105 cites W2019345464 @default.
• W2052544105 cites W2021588424 @default.
• W2052544105 cites W2022181875 @default.
• W2052544105 cites W2023111832 @default.
• W2052544105 cites W2029603526 @default.
• W2052544105 cites W2032730667 @default.
• W2052544105 cites W2035456945 @default.
• W2052544105 cites W2046745157 @default.
• W2052544105 cites W2051872988 @default.
• W2052544105 cites W2051907403 @default.
• W2052544105 cites W2057706762 @default.
• W2052544105 cites W2058711235 @default.
• W2052544105 cites W2062591638 @default.
• W2052544105 cites W2067069532 @default.
• W2052544105 cites W2069671898 @default.
• W2052544105 cites W2070909281 @default.
• W2052544105 cites W2071044636 @default.
• W2052544105 cites W2072008518 @default.
• W2052544105 cites W2076121010 @default.
• W2052544105 cites W2079909894 @default.
• W2052544105 cites W2085296338 @default.
• W2052544105 cites W2087479588 @default.
• W2052544105 cites W2114102323 @default.
• W2052544105 cites W2114840643 @default.
• W2052544105 cites W2121723772 @default.
• W2052544105 cites W2128798413 @default.
• W2052544105 cites W2128831621 @default.
• W2052544105 cites W2129456806 @default.
• W2052544105 cites W2129612249 @default.
• W2052544105 cites W2134540862 @default.
• W2052544105 cites W2140748367 @default.
• W2052544105 cites W2143758907 @default.
• W2052544105 cites W2156395053 @default.
• W2052544105 cites W2162813002 @default.
• W2052544105 cites W2165419075 @default.
• W2052544105 cites W2169069585 @default.
• W2052544105 cites W2171603419 @default.
• W2052544105 cites W2314230416 @default.
• W2052544105 cites W2324375477 @default.
• W2052544105 cites W2394881319 @default.
• W2052544105 doi "https://doi.org/10.1097/tp.0000000000000113" @default.
• W2052544105 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4032218" @default.
• W2052544105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24798312" @default.
• W2052544105 hasPublicationYear "2014" @default.
• W2052544105 type Work @default.
• W2052544105 sameAs 2052544105 @default.
• W2052544105 citedByCount "14" @default.
• W2052544105 countsByYear W20525441052014 @default.
• W2052544105 countsByYear W20525441052015 @default.
• W2052544105 countsByYear W20525441052016 @default.
• W2052544105 countsByYear W20525441052017 @default.
• W2052544105 countsByYear W20525441052018 @default.
• W2052544105 countsByYear W20525441052020 @default.
• W2052544105 countsByYear W20525441052023 @default.
• W2052544105 crossrefType "journal-article" @default.
• W2052544105 hasAuthorship W2052544105A5019013671 @default.
• W2052544105 hasAuthorship W2052544105A5021544227 @default.
• W2052544105 hasAuthorship W2052544105A5031428632 @default.
• W2052544105 hasAuthorship W2052544105A5042295492 @default.
• W2052544105 hasAuthorship W2052544105A5045144667 @default.
• W2052544105 hasAuthorship W2052544105A5056617953 @default.
• W2052544105 hasAuthorship W2052544105A5058862472 @default.
• W2052544105 hasAuthorship W2052544105A5069641539 @default.
• W2052544105 hasBestOaLocation W20525441051 @default.
• W2052544105 hasConcept C104317684 @default.
• W2052544105 hasConcept C137061746 @default.
• W2052544105 hasConcept C147483822 @default.

• next