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- W2052578541 abstract "Abstract Guinea pig adrenal slices were incubated either for 2 h in the presence of 200 μC [1- 14 C]acetate and [7- 3 H]progesterone or for three 2-h periods adding fresh medium with [1- 14 C]acetate to the slices every second hour. In the latter experiments the slices were then incubated for varying times in the presence or absence of [1- 14 C]-acetate and [7- 3 H]progesterone. The addition of ACTH to the incubation medium increased the incorporation of 14 C and 3 H into the adrenal steroids in both long- and short-term experiments. A NADPH-generating system increased the incorporation of both isotopes into adrenal steroids in long-term experiments. In the 2-h experiments, however, the NADPH-generating system decreased the specific activity of [ 14 C]sterols in the adrenal slices. In long-term experiments the steroid response to ACTH and NADPH addition could be maintained over repeated incubations with the same tissues, and ACTH decreased the [ 14 C]cortisol specific activity in such experiments. The data are discussed with regard to the mechanism of action of ACTH." @default.
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- W2052578541 date "1965-07-01" @default.
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- W2052578541 title "Biosynthesis of corticoids in guinea pig adrenal slices the effect of ACTH on [1-14C]acetate incorporation into adrenal steroids" @default.
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- W2052578541 doi "https://doi.org/10.1016/0304-4165(65)90356-9" @default.
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