Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052578550> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2052578550 endingPage "12365" @default.
- W2052578550 startingPage "12358" @default.
- W2052578550 abstract "Heparan sulfate plays a wide range of physiological and pathological roles. Heparan sulfate consists of glucosamine and glucuronic/iduronic acid repeating disaccharides with various sulfations. Synthesis of structurally defined heparan sulfate oligosaccharides remains a challenge. Access to nonsulfated and unepimerized heparan sulfate backbone structures represents an essential step toward de novo enzymatic synthesis of heparan sulfate. The nonsulfated, unepimerized backbone heparan sulfate is similar to the capsular polysaccharide from Escherichia coli strain K5. The biosynthesis of this capsular polysaccharide involves in N-acetylglucosaminyltransferase (KfiA) and d-glucuronyltransferase (KfiC). In this study, we report the characterization of purified KfiA. KfiA was expressed in a C-terminal six-His fusion protein in BL21 star cells coexpressing chaperone proteins GroEL and GroES. The recombinant KfiA was purified to homogeneity with a Ni−agarose column. The binding affinities of various UDP-sugars for KfiA were determined using isothermal calorimetry titration, indicating that both the N-acetyl group and sugar type may be essential for donor substrates to bind KfiA. Kinetic analysis of KfiA toward different sizes of oligosaccharide revealed that KfiA is less sensitive to the size of the acceptor substrates. The results from this study open a new approach for the synthesis of the heparan sulfate backbone." @default.
- W2052578550 created "2016-06-24" @default.
- W2052578550 creator A5039896971 @default.
- W2052578550 creator A5052347803 @default.
- W2052578550 creator A5060165452 @default.
- W2052578550 date "2006-09-14" @default.
- W2052578550 modified "2023-09-26" @default.
- W2052578550 title "Determination of the Substrate Specificities of <i>N</i>-Acetyl-<scp>d</scp>-glucosaminyltransferase" @default.
- W2052578550 cites W1941585788 @default.
- W2052578550 cites W1971540677 @default.
- W2052578550 cites W1979361877 @default.
- W2052578550 cites W1989252536 @default.
- W2052578550 cites W2008581828 @default.
- W2052578550 cites W2014549797 @default.
- W2052578550 cites W2093485518 @default.
- W2052578550 cites W2111015751 @default.
- W2052578550 cites W2111065678 @default.
- W2052578550 cites W2149944903 @default.
- W2052578550 cites W2169829977 @default.
- W2052578550 cites W3118986828 @default.
- W2052578550 doi "https://doi.org/10.1021/bi060844g" @default.
- W2052578550 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17014088" @default.
- W2052578550 hasPublicationYear "2006" @default.
- W2052578550 type Work @default.
- W2052578550 sameAs 2052578550 @default.
- W2052578550 citedByCount "49" @default.
- W2052578550 countsByYear W20525785502012 @default.
- W2052578550 countsByYear W20525785502013 @default.
- W2052578550 countsByYear W20525785502014 @default.
- W2052578550 countsByYear W20525785502015 @default.
- W2052578550 countsByYear W20525785502016 @default.
- W2052578550 countsByYear W20525785502017 @default.
- W2052578550 countsByYear W20525785502018 @default.
- W2052578550 countsByYear W20525785502019 @default.
- W2052578550 countsByYear W20525785502020 @default.
- W2052578550 countsByYear W20525785502021 @default.
- W2052578550 countsByYear W20525785502022 @default.
- W2052578550 countsByYear W20525785502023 @default.
- W2052578550 crossrefType "journal-article" @default.
- W2052578550 hasAuthorship W2052578550A5039896971 @default.
- W2052578550 hasAuthorship W2052578550A5052347803 @default.
- W2052578550 hasAuthorship W2052578550A5060165452 @default.
- W2052578550 hasConcept C153074725 @default.
- W2052578550 hasConcept C156911925 @default.
- W2052578550 hasConcept C185592680 @default.
- W2052578550 hasConcept C2777726330 @default.
- W2052578550 hasConcept C2778041096 @default.
- W2052578550 hasConcept C2779650520 @default.
- W2052578550 hasConcept C55493867 @default.
- W2052578550 hasConceptScore W2052578550C153074725 @default.
- W2052578550 hasConceptScore W2052578550C156911925 @default.
- W2052578550 hasConceptScore W2052578550C185592680 @default.
- W2052578550 hasConceptScore W2052578550C2777726330 @default.
- W2052578550 hasConceptScore W2052578550C2778041096 @default.
- W2052578550 hasConceptScore W2052578550C2779650520 @default.
- W2052578550 hasConceptScore W2052578550C55493867 @default.
- W2052578550 hasIssue "40" @default.
- W2052578550 hasLocation W20525785501 @default.
- W2052578550 hasLocation W20525785502 @default.
- W2052578550 hasOpenAccess W2052578550 @default.
- W2052578550 hasPrimaryLocation W20525785501 @default.
- W2052578550 hasRelatedWork W2027074537 @default.
- W2052578550 hasRelatedWork W2052578550 @default.
- W2052578550 hasRelatedWork W2072925005 @default.
- W2052578550 hasRelatedWork W2098391685 @default.
- W2052578550 hasRelatedWork W2165172458 @default.
- W2052578550 hasRelatedWork W2599261875 @default.
- W2052578550 hasRelatedWork W3187300915 @default.
- W2052578550 hasRelatedWork W4211263999 @default.
- W2052578550 hasRelatedWork W641476228 @default.
- W2052578550 hasRelatedWork W79497180 @default.
- W2052578550 hasVolume "45" @default.
- W2052578550 isParatext "false" @default.
- W2052578550 isRetracted "false" @default.
- W2052578550 magId "2052578550" @default.
- W2052578550 workType "article" @default.