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• W2052588538 abstract "The sterile insect technique (SIT) is an environment-friendly method used in area-wide pest management of the Mediterranean fruit fly Ceratitis capitata (Wiedemann; Diptera: Tephritidae). Ionizing radiation used to generate reproductive sterility in the mass-reared populations before release leads to reduction of competitiveness. Here, we present a first alternative reproductive sterility system for medfly based on transgenic embryonic lethality. This system is dependent on newly isolated medfly promoter/enhancer elements of cellularization-specifically-expressed genes. These elements act differently in expression strength and their ability to drive lethal effector gene activation. Moreover, position effects strongly influence the efficiency of the system. Out of 60 combinations of driver and effector construct integrations, several lines resulted in larval and pupal lethality with one line showing complete embryonic lethality. This line was highly competitive to wildtype medfly in laboratory and field cage tests. The high competitiveness of the transgenic lines and the achieved 100% embryonic lethality causing reproductive sterility without the need of irradiation can improve the efficacy of operational medfly SIT programs." @default.
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• W2052588538 date "2009-01-27" @default.
• W2052588538 modified "2023-10-16" @default.
• W2052588538 title "Conditional embryonic lethality to improve the sterile insect technique in Ceratitis capitata(Diptera: Tephritidae)" @default.
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• W2052588538 doi "https://doi.org/10.1186/1741-7007-7-4" @default.
• W2052588538 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2662800" @default.
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