Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052617632> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2052617632 endingPage "445a" @default.
- W2052617632 startingPage "445a" @default.
- W2052617632 abstract "One major challenge in the expression and purification of recombinant proteins is preventing proteins from entering inclusion bodies. While some proteins can be extracted and refolded from the insoluble inclusion bodies others cannot, such as those proteins containing a protein affinity tag. This study will examine those specific challenges and will explore potential solutions to those problems. We aim to exploit properties of osmolytes like betaine, sarcosine, arginine, and proline to design affinity tags that prevent the formation of inclusion bodies during over-expression of proteins in bacterial hosts. Results of the interactions of the designed affinity tag(s) with transition metals, like nickel and cobalt, will be presented. In addition, correlation between recombinant protein expression yields and the length of the affinity tag(s) will also be discussed." @default.
- W2052617632 created "2016-06-24" @default.
- W2052617632 creator A5006322338 @default.
- W2052617632 creator A5048603239 @default.
- W2052617632 creator A5080195101 @default.
- W2052617632 date "2010-01-01" @default.
- W2052617632 modified "2023-09-27" @default.
- W2052617632 title "Design of Novel Affinity Tags to Increase Recovery of Recombinant Proteins in Ther Soluble Forms" @default.
- W2052617632 doi "https://doi.org/10.1016/j.bpj.2009.12.2420" @default.
- W2052617632 hasPublicationYear "2010" @default.
- W2052617632 type Work @default.
- W2052617632 sameAs 2052617632 @default.
- W2052617632 citedByCount "0" @default.
- W2052617632 crossrefType "journal-article" @default.
- W2052617632 hasAuthorship W2052617632A5006322338 @default.
- W2052617632 hasAuthorship W2052617632A5048603239 @default.
- W2052617632 hasAuthorship W2052617632A5080195101 @default.
- W2052617632 hasBestOaLocation W20526176321 @default.
- W2052617632 hasConcept C104317684 @default.
- W2052617632 hasConcept C127160389 @default.
- W2052617632 hasConcept C185592680 @default.
- W2052617632 hasConcept C203750385 @default.
- W2052617632 hasConcept C2777248517 @default.
- W2052617632 hasConcept C2777756961 @default.
- W2052617632 hasConcept C2779943223 @default.
- W2052617632 hasConcept C2780430158 @default.
- W2052617632 hasConcept C40767141 @default.
- W2052617632 hasConcept C515207424 @default.
- W2052617632 hasConcept C55493867 @default.
- W2052617632 hasConcept C6856738 @default.
- W2052617632 hasConcept C70721500 @default.
- W2052617632 hasConcept C86803240 @default.
- W2052617632 hasConceptScore W2052617632C104317684 @default.
- W2052617632 hasConceptScore W2052617632C127160389 @default.
- W2052617632 hasConceptScore W2052617632C185592680 @default.
- W2052617632 hasConceptScore W2052617632C203750385 @default.
- W2052617632 hasConceptScore W2052617632C2777248517 @default.
- W2052617632 hasConceptScore W2052617632C2777756961 @default.
- W2052617632 hasConceptScore W2052617632C2779943223 @default.
- W2052617632 hasConceptScore W2052617632C2780430158 @default.
- W2052617632 hasConceptScore W2052617632C40767141 @default.
- W2052617632 hasConceptScore W2052617632C515207424 @default.
- W2052617632 hasConceptScore W2052617632C55493867 @default.
- W2052617632 hasConceptScore W2052617632C6856738 @default.
- W2052617632 hasConceptScore W2052617632C70721500 @default.
- W2052617632 hasConceptScore W2052617632C86803240 @default.
- W2052617632 hasIssue "3" @default.
- W2052617632 hasLocation W20526176321 @default.
- W2052617632 hasOpenAccess W2052617632 @default.
- W2052617632 hasPrimaryLocation W20526176321 @default.
- W2052617632 hasRelatedWork W2044103721 @default.
- W2052617632 hasRelatedWork W2052617632 @default.
- W2052617632 hasRelatedWork W2113800550 @default.
- W2052617632 hasRelatedWork W2293347756 @default.
- W2052617632 hasRelatedWork W2379597254 @default.
- W2052617632 hasRelatedWork W2380554478 @default.
- W2052617632 hasRelatedWork W2385248168 @default.
- W2052617632 hasRelatedWork W2739605023 @default.
- W2052617632 hasRelatedWork W2978140776 @default.
- W2052617632 hasRelatedWork W4366084312 @default.
- W2052617632 hasVolume "98" @default.
- W2052617632 isParatext "false" @default.
- W2052617632 isRetracted "false" @default.
- W2052617632 magId "2052617632" @default.
- W2052617632 workType "article" @default.