Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052806033> ?p ?o ?g. }
- W2052806033 endingPage "304" @default.
- W2052806033 startingPage "294" @default.
- W2052806033 abstract "CCR5 and CXCR4 are the main coreceptors for non-syncytia-inducing (NSI) and syncytia-inducing (SI) HIV-1 strains, respectively. NSI HIV-1 isolates do not infect either human lymphoid or monocytoid cell lines, and this inability correlates with the absence of CCR5 expression in these cell types. The ability of SI HIV-1 isolates to infect human primary macrophages has been disputed. Here, we report that CXCR4 is expressed in primary blood-derived human mononuclear phagocytes at all stages of differentiation, although the maturation process correlates with downregulation of CXCR4 mRNA. Infection experiments with the SI molecular clone NL4-3 tagged with a mutant of the green fluorescent protein established that both monocytes and attached macrophages are susceptible to infection with CXCR4-restricted HIV-1 strains. NL4-3 entry into primary macrophages could be blocked by SDF-1alpha in a dose-dependent manner, or by the anti-CXCR4 monoclonal antibody 12G5. HIV-1 entry led to productive infection. No evidence of postentry defects or nuclear import delay for CXCR4-restricted HIV-1 strains was detected using a quantitative real-time PCR assay measuring HIV-1 DNA entry into the nucleus. Macrophages infected by HIV-1 and expressing virus were maintained in culture for long periods of time (up to 5 months). These results demonstrate that CXCR4 is the main HIV-1 SI coreceptor in human primary macrophages and underline the importance of the macrophage as a long-living viral reservoir for HIV-1." @default.
- W2052806033 created "2016-06-24" @default.
- W2052806033 creator A5007135806 @default.
- W2052806033 creator A5009240916 @default.
- W2052806033 creator A5013584826 @default.
- W2052806033 creator A5066261446 @default.
- W2052806033 creator A5070615799 @default.
- W2052806033 date "2000-04-01" @default.
- W2052806033 modified "2023-09-27" @default.
- W2052806033 title "CXCR4 Mediates Entry and Productive Infection of Syncytia-Inducing (X4) HIV-1 Strains in Primary Macrophages" @default.
- W2052806033 cites W100549942 @default.
- W2052806033 cites W1490928025 @default.
- W2052806033 cites W1564316553 @default.
- W2052806033 cites W1659719610 @default.
- W2052806033 cites W1664573657 @default.
- W2052806033 cites W1747721019 @default.
- W2052806033 cites W1817018401 @default.
- W2052806033 cites W1840516913 @default.
- W2052806033 cites W1879790343 @default.
- W2052806033 cites W1881691580 @default.
- W2052806033 cites W1906826083 @default.
- W2052806033 cites W1932752516 @default.
- W2052806033 cites W1938763205 @default.
- W2052806033 cites W1963855922 @default.
- W2052806033 cites W1964384235 @default.
- W2052806033 cites W1965019797 @default.
- W2052806033 cites W1970664121 @default.
- W2052806033 cites W1975625627 @default.
- W2052806033 cites W1981440980 @default.
- W2052806033 cites W1981644527 @default.
- W2052806033 cites W1986751634 @default.
- W2052806033 cites W1987774953 @default.
- W2052806033 cites W1989035987 @default.
- W2052806033 cites W1996183144 @default.
- W2052806033 cites W1998990074 @default.
- W2052806033 cites W2004319639 @default.
- W2052806033 cites W2025921582 @default.
- W2052806033 cites W2026608391 @default.
- W2052806033 cites W2026710381 @default.
- W2052806033 cites W2029723920 @default.
- W2052806033 cites W2030692398 @default.
- W2052806033 cites W2030889255 @default.
- W2052806033 cites W2033975271 @default.
- W2052806033 cites W2075416959 @default.
- W2052806033 cites W2077219778 @default.
- W2052806033 cites W2085464725 @default.
- W2052806033 cites W2088907297 @default.
- W2052806033 cites W2095651361 @default.
- W2052806033 cites W2097183306 @default.
- W2052806033 cites W2099982660 @default.
- W2052806033 cites W2106804406 @default.
- W2052806033 cites W2126255146 @default.
- W2052806033 cites W2131376910 @default.
- W2052806033 cites W2149579640 @default.
- W2052806033 cites W2152003330 @default.
- W2052806033 cites W2153176056 @default.
- W2052806033 cites W2165627434 @default.
- W2052806033 cites W2166323167 @default.
- W2052806033 cites W2167353442 @default.
- W2052806033 cites W2168325635 @default.
- W2052806033 cites W4255745456 @default.
- W2052806033 cites W4313348020 @default.
- W2052806033 doi "https://doi.org/10.1006/viro.1999.0136" @default.
- W2052806033 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10753708" @default.
- W2052806033 hasPublicationYear "2000" @default.
- W2052806033 type Work @default.
- W2052806033 sameAs 2052806033 @default.
- W2052806033 citedByCount "43" @default.
- W2052806033 countsByYear W20528060332014 @default.
- W2052806033 countsByYear W20528060332015 @default.
- W2052806033 countsByYear W20528060332017 @default.
- W2052806033 countsByYear W20528060332019 @default.
- W2052806033 countsByYear W20528060332021 @default.
- W2052806033 countsByYear W20528060332022 @default.
- W2052806033 countsByYear W20528060332023 @default.
- W2052806033 crossrefType "journal-article" @default.
- W2052806033 hasAuthorship W2052806033A5007135806 @default.
- W2052806033 hasAuthorship W2052806033A5009240916 @default.
- W2052806033 hasAuthorship W2052806033A5013584826 @default.
- W2052806033 hasAuthorship W2052806033A5066261446 @default.
- W2052806033 hasAuthorship W2052806033A5070615799 @default.
- W2052806033 hasBestOaLocation W20528060331 @default.
- W2052806033 hasConcept C104317684 @default.
- W2052806033 hasConcept C106358424 @default.
- W2052806033 hasConcept C129470790 @default.
- W2052806033 hasConcept C13373296 @default.
- W2052806033 hasConcept C137061746 @default.
- W2052806033 hasConcept C143065580 @default.
- W2052806033 hasConcept C159047783 @default.
- W2052806033 hasConcept C159654299 @default.
- W2052806033 hasConcept C202751555 @default.
- W2052806033 hasConcept C203014093 @default.
- W2052806033 hasConcept C2522874641 @default.
- W2052806033 hasConcept C2779244956 @default.
- W2052806033 hasConcept C40479148 @default.
- W2052806033 hasConcept C542903549 @default.
- W2052806033 hasConcept C54355233 @default.
- W2052806033 hasConcept C81089528 @default.