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- W2052809504 abstract "A fast and sensitive high performance liquid chromatography (HPLC) assay was developed on a C18 monolithic column for the simultaneous determination of acetaminophen–caffeine–butalbital in human serum. Serum samples were treated with a solid phase extraction procedure. The analytes were separated using a mobile phase of 95:5 (v/v) 0.1 M potassium phosphate monobasic (pH 2.41)–acetonitrile on the C18 monolithic column with detection at 220 nm. Benzoic acid was used as the internal standard (IS). The method was validated over the range of 1.25–100 μg/ml for each drug and found to be linear (r > 0.995, n = 12) with RSD less than 8.3%. The method proved to be accurate (percent bias for all calibration samples varied from −14.6 to −1.3%) and precise (ranged from 2.9 to 13.4%). The mean percent absolute recoveries from serum were 89.7 ± 3.6 for acetaminophen, 95.5 ± 4.5 for caffeine, 99 ± 5.2 for butalbital and 83.4 ± 3.9% for the internal standard." @default.
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- W2052809504 date "2004-11-01" @default.
- W2052809504 modified "2023-09-23" @default.
- W2052809504 title "Assay for the simultaneous determination of acetaminophen–caffeine–butalbital in human serum using a monolithic column" @default.
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- W2052809504 doi "https://doi.org/10.1016/j.jpba.2004.07.042" @default.
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