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• W2052843505 abstract "Phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulates at the leading edge of migrating cells and works, at least partially, as both a compass to indicate directionality and a hub for subsequent intracellular events. However, how PtdIns(3,4,5)P3 regulates the migratory machinery has not been fully elucidated. Here, we demonstrate a novel mechanism for efficient lamellipodium formation that depends on PtdIns(3,4,5)P3 and the reciprocal regulation of PtdIns(3,4,5)P3 itself. LL5β, whose subcellular localization is directed by membrane PtdIns(3,4,5)P3, recruits the actin-cross-linking protein Filamin A to the plasma membrane, where PtdIns(3,4,5)P3 accumulates, with the Filamin A-binding Src homology 2 domain-containing inositol polyphosphate 5-phosphatase 2 (SHIP2). A large and dynamic lamellipodium was formed in the presence of Filamin A and LL5β by the application of epidermal growth factor. Conversely, depletion of either Filamin A or LL5β or the overexpression of either an F-actin-cross-linking mutant of Filamin A or a mutant of LL5β without its PtdIns(3,4,5)P3-interacting region inhibited such events in COS-7 cells. Because F-actin initially polymerizes near the plasma membrane, it is likely that membrane-recruited Filamin A efficiently cross-links newly polymerized F-actin, leading to enhanced lamellipodium formation at the site of PtdIns(3,4,5)P3 accumulation. Moreover, we demonstrate that co-recruited SHIP2 dephosphorylates PtdIns(3,4,5)P3 at the same location. Phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulates at the leading edge of migrating cells and works, at least partially, as both a compass to indicate directionality and a hub for subsequent intracellular events. However, how PtdIns(3,4,5)P3 regulates the migratory machinery has not been fully elucidated. Here, we demonstrate a novel mechanism for efficient lamellipodium formation that depends on PtdIns(3,4,5)P3 and the reciprocal regulation of PtdIns(3,4,5)P3 itself. LL5β, whose subcellular localization is directed by membrane PtdIns(3,4,5)P3, recruits the actin-cross-linking protein Filamin A to the plasma membrane, where PtdIns(3,4,5)P3 accumulates, with the Filamin A-binding Src homology 2 domain-containing inositol polyphosphate 5-phosphatase 2 (SHIP2). A large and dynamic lamellipodium was formed in the presence of Filamin A and LL5β by the application of epidermal growth factor. Conversely, depletion of either Filamin A or LL5β or the overexpression of either an F-actin-cross-linking mutant of Filamin A or a mutant of LL5β without its PtdIns(3,4,5)P3-interacting region inhibited such events in COS-7 cells. Because F-actin initially polymerizes near the plasma membrane, it is likely that membrane-recruited Filamin A efficiently cross-links newly polymerized F-actin, leading to enhanced lamellipodium formation at the site of PtdIns(3,4,5)P3 accumulation. Moreover, we demonstrate that co-recruited SHIP2 dephosphorylates PtdIns(3,4,5)P3 at the same location. Directed migration is crucial in many biological events, including morphogenesis during development, accumulation of immune cells to the site of infection, and metastasis of cancer cells. A lamellipodium (a sheet-like process composed of actin filaments) must be formed correctly at the leading edge of a cell for smooth and efficient migration. Following stimulation with a chemoattractant, phosphatidylinositol 3-kinase (PI3K) 6The abbreviations used are: PI3Kphosphatidylinositol 3-kinasePtdInsphosphatidylinositolPtdIns(3,4,5)P3phosphatidylinositol 3,4,5-triphosphateABDactin-binding domain of Filamin AARNOARF nucleotide-binding site openerPHpleckstrin homologyFRfilamin repeatsCOS-7African green monkey SV40-transfected kidney fibroblast cell lineEGFepidermal growth factorGFPgreen fluorescent proteinEGFPenhanced green fluorescent proteinF-actinactin filamentHAhemagglutininSHIP2Src homology 2 domain-containing inositol polyphosphate 5-phosphatase 2MALDI-TOFmatrix-assisted laser desorption ionization/time of flightRNAiRNA interferenceshRNAshort hairpin RNAVSVvesicular stomatitis virus. is locally activated, resulting in the transient accumulation of PtdIns(3,4,5)P3 on the leading edge of directed migrating amoebas and leukocytes (1Merlot S. Firtel R.A. J. Cell Sci. 2003; 116: 3471-3478Crossref PubMed Scopus (237) Google Scholar). PtdIns(3,4,5)P3 works, at least in part, as a cell compass that translates external signals into directed cell movement (2Franca-Koh J. Kamimura Y. Devreotes P.N. Nat. Cell Biol. 2007; 9: 15-17Crossref PubMed Scopus (54) Google Scholar). However, how PtdIns(3,4,5)P3 contributes to the appropriate formation of a lamellipodium, or conversely, how lamellipodium formation influences PtdIns(3,4,5)P3 accumulation has not been fully elucidated. For proper lamellipodium formation, the intracellular network of actin filaments (F-actins) must be regulated both dynamically and precisely. Such networks are composed primarily of cross-linked and branched F-actins. Previous work has shown that Filamin A cross-links F-actins and is indispensable for lamellipodium formation, whereas the Wiskott-Aldrich syndrome protein family verprolin homologous (WAVE) proteins are involved in branch formation (3Gorlin J.B. Yamin R. Egan S. Stewart M. Stossel T.P. Kwiatkowski D.J. Hartwig J.H. J. Cell Biol. 1990; 111: 1089-1105Crossref PubMed Scopus (434) Google Scholar, 4Cunningham C.C. Gorlin J.B. Kwiatkowski D.J. Hartwig J.H. Janmey P.A. Byers H.R. Stossel T.P. Science. 1992; 255: 325-327Crossref PubMed Scopus (498) Google Scholar, 5Takenawa T. Suetsugu S. Nat. Rev. Mol. Cell Biol. 2007; 8: 37-48Crossref PubMed Scopus (712) Google Scholar). phosphatidylinositol 3-kinase phosphatidylinositol phosphatidylinositol 3,4,5-triphosphate actin-binding domain of Filamin A ARF nucleotide-binding site opener pleckstrin homology filamin repeats African green monkey SV40-transfected kidney fibroblast cell line epidermal growth factor green fluorescent protein enhanced green fluorescent protein actin filament hemagglutinin Src homology 2 domain-containing inositol polyphosphate 5-phosphatase 2 matrix-assisted laser desorption ionization/time of flight RNA interference short hairpin RNA vesicular stomatitis virus. Proteins involved in signal transduction and cytoskeletal dynamics interact with membrane phosphoinositides through their pleckstrin homology (PH) domains; such interactions are critical for the subcellular localization of these proteins and the regulation of their activities (6Harlan J.E. Hajduk P.J. Yoon H.S. Fesik S.W. Nature. 1994; 371: 168-170Crossref PubMed Scopus (678) Google Scholar, 7Lemmon M.A. Ferguson K.M. Biochem. J. 2000; 350: 1-18Crossref PubMed Scopus (618) Google Scholar). Although the core tertiary structure is conserved among PH domains, high sequence variability among their primary structures (∼120 amino acids) gives rise to different specificities of PH domains for different phosphoinositides (8Franke T.F. Kaplan D.R. Cantley L.C. Toker A. Science. 1997; 275: 665-668Crossref PubMed Scopus (1310) Google Scholar, 9Itoh T. Takenawa T. Cell. Signal. 2002; 14: 733-743Crossref PubMed Scopus (117) Google Scholar). LL5β (PHLDB2; pleckstrin homology-like domain, family B, member 2) is an ∼160-kDa protein that contains two predicted coiled-coil domains and a PH domain that is highly specific for PtdIns(3,4,5)P3 (10Levi L. Hanukoglu I. Raikhinstein M. Kohen F. Koch Y. Biochim. Biophys. Acta. 1993; 1216: 342-344Crossref PubMed Scopus (9) Google Scholar, 11Paranavitane V. Coadwell W.J. Eguinoa A. Hawkins P.T. Stephens L. J. Biol. Chem. 2003; 278: 1328-1335Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar). It has been reported that LL5β binds to exogenous Filamin A, as well as Filamin C (12Paranavitane V. Stephens L.R. Hawkins P.T. Cell. Signal. 2007; 19: 817-824Crossref PubMed Scopus (10) Google Scholar), in vitro. However, the LL5β and Filamin A regions required for binding and the biological significance of LL5β-Filamin A binding have not been experimentally explored. It has been shown that SHIP2 is a critical component of a negative feedback loop that regulates PtdIns(3,4,5)P3 levels in nerve growth factor-stimulated PC12 cells, together with the constitutively active phosphatidyl inositol-3 (PtdIns-3) phosphatase, phosphatase and tensin homologue deleted on chromosome 10 (PTEN) (13Aoki K. Nakamura T. Inoue T. Meyer T. Matsuda M. J. Cell Biol. 2007; 177: 817-827Crossref PubMed Scopus (61) Google Scholar). This suggests that SHIP2 contributes to establishing a narrow accumulation of PtdIns(3,4,5)P3 in the membrane. Because PtdIns(3,4,5)P3 works as an internal compass of polarity in directed migrating cells, SHIP2 is likely to help a cell respond accurately and dynamically to external signals that impart directionality by increasing PtdIns(3,4,5)P3 levels in a leading edge of the cell. Previous studies have demonstrated that SHIP2 binds to Filamin A and that Filamin A is essential for the translocation of SHIP2 to the membrane ruffle. Moreover, recombinant SHIP2 regulates PtdIns(3,4,5)P3 levels and the distribution of submembranous actin at membrane ruffles following growth factor stimulation (14Dyson J.M. O'Malley C.J. Becanovic J. Munday A.D. Berndt M.C. Coghill I.D. Nandurkar H.H. Ooms L.M. Mitchell C.A. J. Cell Biol. 2001; 155: 1065-1079Crossref PubMed Scopus (140) Google Scholar). However, it remains elusive as to how and what molecules control SHIP2 and Filamin A translocation to the membrane, where in the cell periphery SHIP2 translocates, how membranous PtdIns(3,4,5)P3 levels are regulated by translocation of SHIP2, and how submembranous actins are controlled. Details of experimental procedures are provided in the supplemental Experimental Procedures. The full-length human LL5β cDNA (GenBankTM accession number AJ496194) was cloned from a human placenta cDNA library (BD Biosciences). Full-length LL5β cDNA was subcloned into pEGFP-C3 (plasmid vector for expressing enhanced green fluorescent protein-C3, Clontech) to generate pEGFP-LL5β. In addition, expression vectors for human LL5β lacking the first 156 amino acid residues (pEGFP-LL5βΔN1, we designated this vector as ΔN1), 323 amino acid residues (pEGFP-LL5βΔN2 as ΔN2), and 488 amino acid residues (pEGFP-LL5βΔN3 as ΔN3) were generated using pEGFP-C3. pEGFP-C3 was also used for enhanced green fluorescent protein (EGFP) expression in COS-7 cells. Procedures for generating ΔN4, ΔN5, Δ2-a to Δ2-e, LL5βΔPH, and LL5β-VSV are described in the supplemental Experimental Procedures. Other protein expression vectors were constructed using pCAGGS so that the cDNAs would be driven under a modified chicken β-actin (CAG) promoter. We used pCAGGS-hemagglutinin (HA)-tagged Filamin A (pCAGGS-HA-Filamin A) (15Nagano T. Morikubo S. Sato M. J. Neurosci. 2004; 24: 9648-9657Crossref PubMed Scopus (124) Google Scholar). The following vectors were also generated: pCAGGS-HA-Filamin A truncated mutant 1 (Filamin AΔ1), pCAGGS-HA-Filamin A truncated mutant 2 (Filamin AΔ2), pCAGGS-HA-Filamin A truncated mutant 3 (Filamin AΔ3), and pCAGGS-HA-Filamin without repeat 24 (Filamin A ΔFR24). In addition, various vectors that expressed Val-Val-Val-Val-Val (V5)-His-tagged Filamin A fragments were constructed: CH1CH2, which contains the actin-binding domain 1 (CH1) and domain 2 (CH2) of Filamin A; FR24, which contains the filamin repeat (FR, also called immunoglobulin-like-β-sheet motif) 24 of Filamin A; and FR23H2, which contains the filamin repeat 23 and hinge 2 (H2) of Filamin A. The generation of Filamin AΔABD (ABD, actin-binding domain) has been described previously (15Nagano T. Morikubo S. Sato M. J. Neurosci. 2004; 24: 9648-9657Crossref PubMed Scopus (124) Google Scholar). For Filamin C expression, we used pBudCE4-Filamin C (made using pZP7-Filamin C, a generous gift from Dr. D. W. Chung, and pBudCE4 from Invitrogen). Full-length rat SHIP2 (GenBank accession number NM_022944) cDNA was subcloned into pCAGGS-GFP or pmCherry-N1 (Clontech) to generate SHIP2-expressing vector, where mCherry is a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein (DsRed). The PH domain of rat ADP-ribosylation factor (ARF) nucleotide-binding size opener (16Várnai P. Bondeva T. Tamás P. Tóth B. Buday L. Hunyady L. Balla T. J. Cell Sci. 2005; 118: 4879-4888Crossref PubMed Scopus (121) Google Scholar, 17Navolanic P.M. Steelman L.S. McCubrey J.A. Int. J. Oncol. 2003; 22: 237-252PubMed Google Scholar) (ARNO, GenBank accession number NM_053911, gift from Dr. T. Balla) was subcloned into pCAGGS-GFP vector to generate EGFP-ARNO/PH-expressing vector. The PH domain of mouse general receptor for phosphoinositides 1 (18Klarlund J.K. Tsiaras W. Holik J.J. Chawla A. Czech M.P. J. Biol. Chem. 2000; 275: 32816-32821Abstract Full Text Full Text PDF PubMed Scopus (123) Google Scholar) (GRP1, GenBank accession number BC035296.1) was subcloned into pEGFP-C1 to generate EGFP-GRP1/PH-expressing vector. The following antibodies were used: anti-GFP antibody that can recognize EGFP (MBL Medical and Biological Laboratories, Nagoya, Japan), anti-Filamin A antibody (MAB1678, Chemicon), anti-HA antibody (sc-805, Santa Cruz Biotechnology, Santa Cruz), anti-α-tubulin (DM1A, Sigma), anti-VSV antibody (P5D4, Sigma), anti-pan-cadherin antibody (CH-19, Sigma), anti-glyceraldehyde-3-phosphate dehydrogenase antibody (14C10, Cell Signaling Technology, Danvers, MA), anti-rabbit IgG (Sc-2027, Santa Cruz Biotechnology), anti-V5 antibody (Invitrogen), anti-β actin antibody (AC-40, Sigma), anti-FLAG antibody (F3165, Sigma), anti-PtdIns(3,4,5)P3 antibody (F3165, NN111.1.1, MBL Medical and Biological Laboratories), Alexa Fluor 568 phalloidin (Invitrogen). Anti-LL5β polyclonal antibody was generated in cooperation with SIGMA Genosys Japan (Ishikari, Japan). COS-7 cells were used. Vectors were transfected using PolyFect transfection reagent (Qiagen, Hilden, Germany) or FuGENE 6 transfection reagent (Roche Diagnostics). To increase PtdIns (3,4,5)P3 in the plasma membrane, cells were cultured without serum for 16–18 h and then cultured with epidermal growth factor (EGF) for 2 min or serum for 10 min. Human melanoma cell lines M2 and A7 were maintained as reported previously (4Cunningham C.C. Gorlin J.B. Kwiatkowski D.J. Hartwig J.H. Janmey P.A. Byers H.R. Stossel T.P. Science. 1992; 255: 325-327Crossref PubMed Scopus (498) Google Scholar). FuGENE HD (Roche Diagnostics) was used to transfect these cells. Conventional protocols were used. Immunoprecipitation was performed with Dynabeads (Dynal, Hamburg, Germany) coated with antibodies specific to rabbit or mouse IgG. Matrix-assisted laser desorption ionization/time of flight (MALDI-TOF) mass spectrometry (Bruker Daltonics, Billerica, MA) was used for protein identification. Three different constructs of LL5β short hairpin RNA (shRNA) were prepared in the mouse U6 snRNA promoter (mU6pro) vector (mU6pro-LL5β-RNAi), which has a mouse U6 promoter (15Nagano T. Morikubo S. Sato M. J. Neurosci. 2004; 24: 9648-9657Crossref PubMed Scopus (124) Google Scholar). Their sequence targets were nucleotides 177–197, 2246–2266, and 3647–3667 of the human LL5β cDNA. These shRNAs were termed LL5β-shRNA1, LL5β-shRNA2, and LL5β-shRNA3 and such vectors were called LL5β-RNAi1, LL5β-RNAi2, and LL5β-RNAi3, respectively. For co-transfection, a molar ratio of 1 (EGFP expression plasmid) to 2 (LL5β-RNAi or empty mU6pro vector) was employed. Empty shRNA vector was used as a control. Overconfluent COS-7 cells were co-transfected with pEGFP-C3 and mU6pro-LL5β-RNAi vector or pEGFP-C3 and control vector. Empty shRNA vector was used as the control vector. After 36–48 h, the confluent cell layer was disrupted followed by a 3-h incubation. After fixing and staining with rhodamine-phalloidin, cells at the defect edge expressing EGFP were observed, and the numbers of cells with or without lamellipodia were counted. For PI3K inhibition experiments, cells were cultured for 18–24 h followed by the addition of wortmannin (Sigma, final concentration 100 nm) or LY294002 (Sigma, final concentration 10 μm). Time-lapse observation was started (time 0) in a CO2 incubation chamber (5% CO2 at 37 °C) fitted onto a confocal microscope. Images were obtained every 1–2 min for 15 min to 1 h. Confocal images or live cell images were captured. Images were obtained using an AxioCam (Carl Zeiss, Oberkochen, Germany). Subcellular fractionation was performed using the ProteoExtract subcellular proteome extraction kit (Calbiochem) in accordance with the manufacturer's instructions. Utilizing the different extraction buffers in the kit, four different fractions were separated. The Triton-insoluble membrane/organelle fractions are shown in Fig. 5E. The amounts of the pan-cadherin (as reference for membrane proteins) and glyceraldehyde-3-phosphate dehydrogenase were used as internal controls. Significant differences, as indicated by p < 0.01, were determined by the non-parametric Mann-Whitney U test or t test. First of all, we experimentally examined the relationship between LL5β and Filamin A (Fig. 1A) and their regions responsible for interaction. We expressed LL5β, tagged with EGFP at its amino terminus (EGFP-LL5β), in COS-7 cells to determine whether LL5β colocalizes with endogenous Filamin A (Fig. 1B). EGFP-LL5β and endogenous Filamin A colocalized primarily at the cell periphery after EGF application (final concentration, 100 ng/ml; Fig. 1B, left and middle columns), as reported previously (11Paranavitane V. Coadwell W.J. Eguinoa A. Hawkins P.T. Stephens L. J. Biol. Chem. 2003; 278: 1328-1335Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar). Endogenous LL5β (supplemental Fig. S1) colocalized with endogenous Filamin A at the cell periphery by serum stimulation (Fig. 1B, right column). Previous work has demonstrated that PI3K is activated by EGF (17Navolanic P.M. Steelman L.S. McCubrey J.A. Int. J. Oncol. 2003; 22: 237-252PubMed Google Scholar) or by serum stimulation (19Oikawa T. Yamaguchi H. Itoh T. Kato M. Ijuin T. Yamazaki D. Suetsugu S. Takenawa T. Nat. Cell Biol. 2004; 6: 420-426Crossref PubMed Scopus (192) Google Scholar). EGFP-tagged ARNO-PH, which specifically binds to PtdIns(3,4,5)P3, has been used as a PtdIns(3,4,5)P3 detector (16Várnai P. Bondeva T. Tamás P. Tóth B. Buday L. Hunyady L. Balla T. J. Cell Sci. 2005; 118: 4879-4888Crossref PubMed Scopus (121) Google Scholar, 17Navolanic P.M. Steelman L.S. McCubrey J.A. Int. J. Oncol. 2003; 22: 237-252PubMed Google Scholar). Two minutes after EGF application, ARNO-PH accumulated at the cell periphery together with endogenous LL5β (Fig. 1C), suggesting that LL5β translocates where PtdIns(3,4,5)P3 localizes. EGF-induced accumulation of PtdIns(3,4,5)P3 at the cell periphery was further confirmed by another PtdIns(3,4,5)P3 detector GRP1/PH (18Klarlund J.K. Tsiaras W. Holik J.J. Chawla A. Czech M.P. J. Biol. Chem. 2000; 275: 32816-32821Abstract Full Text Full Text PDF PubMed Scopus (123) Google Scholar) or immunocytochemical techniques (supplemental Fig. S2). The interaction between LL5β and Filamin A was confirmed by immunoprecipitation. Cell lysates from COS-7 cells expressing EGFP-LL5β, an anti-GFP antibody that is capable of recognizing EGFP, and an anti-Filamin A antibody were used. This anti-Filamin A antibody did not cross-react with Filamin C in lysates from Filamin C-overexpressing COS-7 cells (data not shown). Filamin A, which is ∼280 kDa (3Gorlin J.B. Yamin R. Egan S. Stewart M. Stossel T.P. Kwiatkowski D.J. Hartwig J.H. J. Cell Biol. 1990; 111: 1089-1105Crossref PubMed Scopus (434) Google Scholar, 20Stossel T.P. Condeelis J. Cooley L. Hartwig J.H. Noegel A. Schleicher M. Shapiro S.S. Nat. Rev. Mol. Cell Biol. 2001; 2: 138-145Crossref PubMed Scopus (834) Google Scholar), was co-immunoprecipitated with EGFP-LL5β (Fig. 1D). We then searched for the region of Filamin A responsible for this interaction. Filamin A, which usually exists as a homodimer, contains an amino-terminal actin-binding domain that precedes 24 immunoglobulin-like β-sheet motifs (FR, filamin repeats) (Fig. 1E). The last FR (FR 24) is responsible for homodimerization (20Stossel T.P. Condeelis J. Cooley L. Hartwig J.H. Noegel A. Schleicher M. Shapiro S.S. Nat. Rev. Mol. Cell Biol. 2001; 2: 138-145Crossref PubMed Scopus (834) Google Scholar). Using three deletion mutants of amino-terminally HA-tagged Filamin A that lack different regions of the immunoglobulin-like-β-sheet motifs (Filamin AΔ1-Filamin AΔ3), we identified the domain of Filamin A responsible for its interaction with LL5β (Fig. 1E). Even Filamin AΔ3, which includes hinge 1 but lacks FR1–23, was co-immunoprecipitated with EGFP-LL5β (Fig. 1E). We next assessed the ability of the two actin-binding motifs (CH1, CH2), FR24 and hinge 2 (H2) of Filamin A, to bind to EGFP-LL5β. Two Filamin A fragments, CH1CH2 and FR24, but not FR23H2, were co-immunoprecipitated with EGFP-LL5β (Fig. 1, E and F). Furthermore, HA-tagged Filamin A without actin-binding domain (HA-Filamin A ΔABD) or without FR24 (HA-Filamin A ΔFR24) was co-immunoprecipitated with EGFP-LL5β (Fig. 1, E, G, and H). These results suggest that both the actin-binding domain and the FR24 of Filamin A are responsible for an interaction with LL5β. We next determined which domains of LL5β are required for this interaction (Fig. 2A). First, we generated a mutant of LL5β that lacks the PH domain (LL5βΔPH). HA-tagged Filamin A was co-expressed with EGFP-tagged LL5β (EGFP-LL5β) or EGFP-tagged LL5βΔPH (EGFP-LL5βΔPH) in COS-7 cells followed by lysis and immunoprecipitation with an anti-GFP antibody. Co-immunoprecipitated HA-tagged Filamin A was observed with the similar intensity in lysates from cells co-expressing either EGFP-LL5β or EGFP-LL5βΔPH (Fig. 2B), indicating that the PH domain is not required for binding to Filamin A. We then used various truncated forms of LL5β with N-terminal EGFP tags to search for regions of LL5β responsible for Filamin A binding. A Filamin A interaction occurred with ΔN1 and ΔN2, but not with the ΔN3, ΔN4, or ΔN5 mutants of LL5β, indicating that a domain present in ΔN2 but not ΔN3 is responsible for this interaction. We then further truncated this domain and found that ΔN2-a, but not ΔN2-b or ΔN2-c, could interact. Finally, we discovered that a 33-amino acid region (323–356, ΔN2-e) was sufficient for binding (Fig. 2C). This binding region of LL5β was different from what has been previously documented (12Paranavitane V. Stephens L.R. Hawkins P.T. Cell. Signal. 2007; 19: 817-824Crossref PubMed Scopus (10) Google Scholar). To confirm our observation, we expressed ΔN2-e in COS-7 cells and identified co-immunoprecipitated proteins using MALDI-TOF mass spectrometry. Then, Filamin A was identified (Fig. 2D). We reconfirmed our results. Because the PH domain of LL5β is specifically sensitive to PtdIns(3,4,5)P3 (10Levi L. Hanukoglu I. Raikhinstein M. Kohen F. Koch Y. Biochim. Biophys. Acta. 1993; 1216: 342-344Crossref PubMed Scopus (9) Google Scholar), we next examined whether the recruitment of LL5β to the lamellipodia is driven by PtdIns(3,4,5)P3. Because the phosphorylation state of PtdIns(3,4,5)P3 is regulated by the phosphorylation of phosphatidylinositol 4,5-biphosphate by PI3K, COS-7 cells with exogenous EGFP-LL5β were treated with the PI3K inhibitor, LY294002. After this treatment, EGFP fluorescence was distributed in a punctate manner and colocalized with endogenous Filamin A (Fig. 2E; supplemental Fig. S3; supplemental Video 1). No accumulation of Filamin A was observed at the cell periphery. Our time-lapse study gave us more detailed information on how this redistribution took place. Puncta were formed in COS-7 cells about 14 min after treatment with the PI3K inhibitor, wortmannin (Fig. 2F). The EGFP-LL5β signal was not observed in lamellipodia after this treatment (Fig. 2F). This observation was further investigated by overexpressing EGFP-LL5βΔPH. EGFP-LL5βΔPH did not localize to the plasma membrane but colocalized well with endogenous Filamin A in a punctate distribution in the cytoplasm (Fig. 2G). This result is consistent with the demonstration of LL5βΔPH binding to HA-tagged Filamin A (Fig. 2B). We further examined the spatiotemporal regulation of LL5β in response to PtdIns(3,4,5)P3. We added EGF to the culture medium after serum depletion for 16–18 h and continuously observed changes in EGFP-LL5β localization. Recruitment of EGFP-LL5β to the plasma membrane was first observed ∼80 s after EGF application, and by 112 s, EGFP-LL5β accumulated along the plasma membrane (Fig. 2H; supplemental Video 2). These data suggest that LL5β changes its subcellular localization quickly enough to chase changeable PtdIns(3,4,5)P3 distribution (supplemental Fig. S4 and supplemental Video 3). The ability of LL5β to bind to Filamin A, a protein crucial for cell motility (20Stossel T.P. Condeelis J. Cooley L. Hartwig J.H. Noegel A. Schleicher M. Shapiro S.S. Nat. Rev. Mol. Cell Biol. 2001; 2: 138-145Crossref PubMed Scopus (834) Google Scholar), suggests that LL5β is also involved in motility control. To investigate the function of LL5β, we carried out LL5β knockdown experiments using RNA interference (RNAi). Of the three different shRNA constructs against LL5β, we found that LL5β-RNAi2 and LL5β-RNAi3 suppressed the expression of EGFP-LL5β with similar efficiency (Fig. 3A; supplemental Fig. S5). In the following experiments, we primarily used LL5β-RNAi2 to suppress LL5β expression in COS-7 cells, but LL5β-RNAi3 gave us similar results (supplemental Fig. S6). LL5β-RNAi reduced cell motility when compared with control cells under low cell density conditions where cells could move freely (Fig. 3, B and C). We then employed a wound-healing assay to determine whether there was any alteration to the cell-crawling machinery (Fig. 3, D and E). In this assay, quiescent cells in an overconfluent state usually develop lamellipodia in response to the removal of neighboring cells. We found that “typical lamellipodia” (defined as edge lines equal to or more than 10 μm long) were not properly formed in cells with LL5β-RNAi. Instead, small protrusions that contained F-actin (with edge lines less than 10 μm long) were observed (Fig. 3D). The frequency of cells with lamellipodia was estimated with or without LL5β-RNAi. Knockdown of LL5β resulted in a decrease in lamellipodium formation (Fig. 3E), suggesting that LL5β is essential for lamellipodium formation in COS-7 cells. Moreover, when LL5β expression was down-regulated by the RNAi technique in COS-7 cells, the distribution of Filamin A near the plasma membrane was significantly altered (Fig. 3F). Reduced formation of lamellipodia and a fiber-like distribution of Filamin A were observed in the small protrusions seen in LL5β knockdown cells. In contrast, LL5β overexpression resulted in the formation of large lamellipodia (Figs. 3G, upper row, and 4A, left and middle panels). In response to EGF, COS-7 cells with exogenous LL5β became very active (supplemental Video 4), whereas those with exogenous LL5βΔPH did not (Fig. 3G, lower row), indicating that the PH domain of LL5β is critical for this action. Because the PH domain of LL5β is primarily sensitive to PtdIns(3,4,5)P3(10Levi L. Hanukoglu I. Raikhinstein M. Kohen F. Koch Y. Biochim. Biophys. Acta. 1993; 1216: 342-344Crossref PubMed Scopus (9) Google Scholar, 11Paranavitane V. Coadwell W.J. Eguinoa A. Hawkins P.T. Stephens L. J. Biol. Chem. 2003; 278: 1328-1335Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar) and because PtdIns(3,4,5)P3 is generated at the plasma membrane upon EGF application (17Navolanic P.M. Steelman L.S. McCubrey J.A. Int. J. Oncol. 2003; 22: 237-252PubMed Google Scholar), it is probable that the binding of LL5β to membrane PtdIns(3,4,5)P3 induced this cellular response.FIGURE 4Filamin A is necessary for induction of lamellipodium formation by LL5β. A, EGFP expression vector or EGFP-LL5β expression vector was transfected into COS-7 cells, A7 cells (which contain Filamin A), and M2 cells (which lack Filamin A). Very large lamellipodia were developed in the presence of EGFP-LL5β in COS-7 cells and A7 cells but not in the M2 cells. Scale bar = 10 μm. B, a whole-cell area was measured from 50 cells; cells were randomly selected in each case (mean ± S.E.). * p < 0.01. C and D, M2 cells expressing EGFP-LL5β and Filamin AΔABD or EGFP and Filamin AΔABD were cultured without serum for 18 h. EGF (final concentration 100 ng/ml) was then added into the culture medium (time 0). Approximately 2 min after EGF addition, small pseudopods (arrowheads) were transiently generated. The images were merged with their differential interference contrast images. Scale bars = 10 μm.View Large Image Figure ViewerDownload Hi-res image Download (PPT) Because LL5β overexpression in COS-7 cells resulted in the enlargement of the peripheral area (including lamellipodia) of cells, we next examined whether Filamin A is involved in this response. M2 cells, in which LL5β mRNA was not detected (data not shown), are derived from a human melanoma and do not express Filamin A, whereas A7 cells are generated by stably transfecting Filamin A cDNA into M2 cells (4Cunningham C.C. Gorlin J.B. Kwiatkowski D.J. Hartwig J.H. Janmey P.A. Byers H.R. Stossel T.P. Science. 1992; 255: 325-327Crossref PubMed Scopus (498) Google Scholar). These cells express a molar ratio of Filamin A to actin comparable with that found in cells normally expressing Filamin A (4Cunningham C.C. Gorlin J.B. Kwiatkowski D.J. Hartwig J.H. Janmey P.A. Byers H.R. Stossel T.P. Science. 1992; 255: 325-327Crossref PubMed Scopus (498) Google Scholar). The LL5β-induced enlargement was observed in A7 cells as well as COS-7 cells, but not in M2 cells (Fig. 4, A and B), suggesting that enlargement induced by LL5β overexpression requires Filamin A. The versatility of Filamin A led us to ask whether the F-actin-cross-linking activity of Filamin A is essential for this enlargement. Filamin A dimerized through their carboxyl termini, and then dimerized Filamin A cross-linked two F-actin molecules through their ABD at their amino termini (15Nagano T. Morikubo S. Sato M. J. Neurosci. 2004; 24: 9648-9657Crossref PubMed Scopus (124) Google Scholar, 21van der Flier A. Sonnenberg A. Biochim. Biophys. Acta. 2001; 1538: 99-117Crossref PubMed Scopus (335) Google Scholar). Thus, we transfected a Filamin AΔABD expression vector (15Nagano T. Morikubo S. Sato M. J. Neurosci. 2004; 24: 9648-9657Crossref PubMed Scopus (124) Google Scholar) into M2 cells and examined the Filamin A F-actin-cross-linking activity to that observed in A7 cells. Application of EGF resulted in the formation of small pseudopods, but not proper lamellipodia, in M2 cells transfected with EGFP-LL5β and Filamin AΔABD (Fig. 4C). Therefore, the actin binding activity, or probably the F-actin-cross-linking activity, of Filamin A is crucial for the LL5β-mediated formation of lamellipodia. Because EGF induced small pseudopods in M2 cells transfected with Filamin AΔABD even in the absence of LL5β (Fig. 4D), it is unlikely that LL5β is involved in the formation of these small pseudopods. We next sought to determine whether LL5β directs translocation of SHIP2-Filamin A complex. As reported (14Dyson J.M. O'Malley C.J. Becanovic J. Munday A.D. Berndt M.C. Coghill I.D. Nandurkar H.H. Ooms L.M. Mitchell C.A. J. Cell Biol. 2001; 155: 1065-1079Crossref PubMed Scopus (140) Google Scholar), SHIP2 interacts with Filamin A (data not shown). By contrast, direct interaction between SHIP2 and LL5β was not observed (supplemental Fig. S7), suggesting that LL5β directs subcellular localization of SHIP2 via Filamin A. Two minutes after EGF stimulation, EGFP-tagged SHIP2 (EGFP-SHIP2) or mCherry-tagged SHIP2 (SHIP2-mCherry) localized to the cell periphery with LL5β (Fig. 5, A, lower row, and B, upper row) but remained in the cytosol in the presence of LL5βΔPH (Fig. 5B, lower row). As expected, SHIP2 colocalized well with Filamin A (Fig. 5C, upper row), but translocation of SHIP2 to the cell membrane was observed less in LL5β knockdown COS-7 cells (Fig. 5, C, lower row, and D). Supporting this observation, the amount of SHIP2 in the membrane fraction was reduced in LL5β knockdown COS-7 cells (Fig. 5E). These data indicate that Filamin A is an intermediate between LL5β and SHIP2. Indeed, translocation of EGFP-SHIP2 and LL5β to the cell periphery was observed in A7 cells (Fig. 5F). In M2 cells (Filamin A-deficient A7 cells), LL5β was translocated to the cell periphery, leaving SHIP2 in the cytosol, following EGF stimulation (Fig. 5G). It was of interest that the amount of α-tubulin was also decreased in LL5β knockdown cells (Fig. 5E). Because it has been reported that SHIP2 regulates PtdIns(3,4,5)P3 at membrane ruffles (14Dyson J.M. O'Malley C.J. Becanovic J. Munday A.D. Berndt M.C. Coghill I.D. Nandurkar H.H. Ooms L.M. Mitchell C.A. J. Cell Biol. 2001; 155: 1065-1079Crossref PubMed Scopus (140) Google Scholar) and because it has also been proposed that SHIP2 mediates negative feedback on the accumulation of PtdIns(3,4,5)P3 (13Aoki K. Nakamura T. Inoue T. Meyer T. Matsuda M. J. Cell Biol. 2007; 177: 817-827Crossref PubMed Scopus (61) Google Scholar), we examined whether SHIP2, which is directed by LL5β through Filamin A, regulates membrane PtdIns(3,4,5)P3. We found that ARNO-PH, one of the widely used PtdIns(3,4,5)P3 detectors (16Várnai P. Bondeva T. Tamás P. Tóth B. Buday L. Hunyady L. Balla T. J. Cell Sci. 2005; 118: 4879-4888Crossref PubMed Scopus (121) Google Scholar, 17Navolanic P.M. Steelman L.S. McCubrey J.A. Int. J. Oncol. 2003; 22: 237-252PubMed Google Scholar) (supplemental Figs. S2 and S4 and supplemental Video 3), showed a greater accumulation at the plasma membrane in LL5β knockdown cells (Fig. 5, H and I). This suggests that SHIP2, which is directed by LL5β, is very likely to regulate membrane PtdIns(3,4,5)P3. We demonstrate here that LL5β is essential for quick Filamin A-dependent SHIP2 translocation in response to membrane PtdIns(3,4,5)P3, fast enough to chase PtdIns(3,4,5)P3 changes, and the reciprocal control of membrane PtdIns(3,4,5)P3 through SHIP2, as well as the enhanced lamellipodium formation induced by PtdIns(3,4,5)P3 (Fig. 6). It has been reported that v-ral simian leukemia viral oncogene homolog A (RalA) targets Filamin A in filopodia (22Ohta Y. Suzuki N. Nakamura S. Hartwig J.H. Stossel T.P. Proc. Natl. Acad. Sci. U.S.A. 1999; 96: 2122-2128Crossref PubMed Scopus (376) Google Scholar). In addition, faint membrane localization of mutated SHIP2 that lacks Filamin A-interacting domain, is observed after 5 min of EGF stimulation (14Dyson J.M. O'Malley C.J. Becanovic J. Munday A.D. Berndt M.C. Coghill I.D. Nandurkar H.H. Ooms L.M. Mitchell C.A. J. Cell Biol. 2001; 155: 1065-1079Crossref PubMed Scopus (140) Google Scholar). This is consistent with the fact that SHIP2 is capable of associating with p130Cas and localizes to membrane ruffles and focal adhesions (23Prasad N. Topping R.S. Decker S.J. Mol. Cell. Biol. 2001; 21: 1416-1428Crossref PubMed Scopus (120) Google Scholar). Because F-actin primarily grows near the plasma membrane by polymerizing globular actins at its plus end (24Watanabe N. Mitchison T.J. Science. 2002; 295: 1083-1086Crossref PubMed Scopus (371) Google Scholar) and because cells do not form lamellipodia (large protrusions) in response to EGF application in the presence of the non-membrane-associating LL5βΔPH mutant, it is likely that increasing the cross-linking of newly polymerized F-actins by membrane-recruited Filamin A helps a cell to form lamellipodia. It is of interest that Filamin A can regulate the formation of small membrane protrusions without its actin-cross-linking activity. Indeed, small pseudopods were formed in M2 cells transfected with Filamin AΔABD in response to EGF. LL5β, as well as Filamin A, is apparently versatile because the amount of α-tubulin in the membrane fraction was decreased by LL5β knockdown. It has been reported that LL5β binds to cytoplasmic linker-associated proteins (CLASPs) (25Lee H. Engel U. Rusch J. Scherrer S. Sheard K. Van Vactor D. Neuron. 2004; 42: 913-926Abstract Full Text Full Text PDF PubMed Scopus (137) Google Scholar), which attach to the plus ends of microtubules, thereby modulating microtubule stabilization (26Lansbergen G. Grigoriev I. Mimori-Kiyosue Y. Ohtsuka T. Higa S. Kitajima I. Demmers J. Galjart N. Houtsmuller A.B. Grosveld F. Akhmanova A. Dev. Cell. 2006; 11: 21-32Abstract Full Text Full Text PDF PubMed Scopus (229) Google Scholar). Therefore, it is possible that LL5β synchronizes actins and microtubules, thereby coordinating cytoskeletal components for efficient migration. It has been shown that SHIP2 is a critical component of a negative feedback loop that regulates PtdIns(3,4,5)P3 levels and is likely to help a cell respond accurately and dynamically to external signals that impart directionality (13Aoki K. Nakamura T. Inoue T. Meyer T. Matsuda M. J. Cell Biol. 2007; 177: 817-827Crossref PubMed Scopus (61) Google Scholar). We show here that SHIP2 translocates directly to sites of PtdIns(3,4,5)P3 accumulation with Filamin A, which is dependent on LL5β. Considering that LL5β accumulates to the membrane in response to EGF application within a few minutes, it is plausible that SHIP2 follows changes in PtdIns(3,4,5)P3 without a substantial delay. Therefore, it is likely that SHIP2, Filamin A, and LL5β help a cell to move in one direction smoothly. Moreover, the ability of PtdIns(3,4,5)P3 and SHIP2 to regulate various cellular responses, such as the insulin response (27Kagawa S. Soeda Y. Ishihara H. Oya T. Sasahara M. Yaguchi S. Oshita R. Wada T. Tsuneki H. Sasaoka T. Endocrinology. 2008; 149: 642-650Crossref PubMed Scopus (52) Google Scholar), and the reciprocal regulation of PtdIns(3,4,5)P3 with Filamin A-LL5β reported here suggest that these findings are significant for the general understanding of such events, and possibly, for the understanding of related diseases. We thank T. Bando, H. Yoshikawa, K. Hisazaki, and T. Taniguchi for technical and secretarial assistance and Y. Ohta, T. P. Stossel, and Y. Yokota for critical reading. We are also grateful to T. Sasaoka for discussions about SHIP2, Y. Ohta and T. P. Stossel for A7 and M2 cells, D. W. Chung for providing human Filamin C cDNA, J. Miyazaki for the pCAGGS vector, T. Balla for ARNO/PH, and J. Y. Yu and D. L. Turner for the mU6pro vector. Download .zip (10.28 MB) Help with zip files" @default.
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• W2052843505 title "LL5β Directs the Translocation of Filamin A and SHIP2 to Sites of Phosphatidylinositol 3,4,5-Triphosphate (PtdIns(3,4,5)P3) Accumulation, and PtdIns(3,4,5)P3 Localization Is Mutually Modified by Co-recruited SHIP2" @default.
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