Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052853780> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2052853780 endingPage "131" @default.
- W2052853780 startingPage "122" @default.
- W2052853780 abstract "Sonicated liposomes of soybean phospholipids (asolectin) distribute nearly throughout a 19–22% (v/v) glycerol gradient when centrifuged to near equilibrium. Upon recentrifugation on an identical gradient, liposomes selected from several positions in such a gradient migrate as narrow bands to positions close to their original positions, indicating that the liposome distribution in the first gradient is the result of a density-based fractionation. Molecular sieve chromatography, turbidity, and trapped volume measurements indicate that the liposome densities are qualitatively related to their size, with the larger liposomes more dense than the smaller ones. Size estimates obtained by electron microscopy of negatively stained preparations indicate that the fractionation is effective for liposomes with diameters ranging from 200 to 600 Å, with maximum efficiency in the range 200–300 Å where the majority of the liposomes is found. Interestingly, high concentrations of liposomes improve the efficiency of the fractionation procedure. The size dependence of liposome density is shown not to be due to differential glycerol permeability or lipid composition, and is therefore most likely due to variations in the specific volumes of the individual phospholipid molecules owing to the curvature of the liposomes. Finally, freezing of the glycerol gradient fractions in liquid N2 and storage at −70°C does not modify the size of the isolated liposomes. It is suggested that glycerol density gradient fractionation of liposomes could be a useful general method for obtaining liposomes of reasonably uniform size in large quantities and high concentrations." @default.
- W2052853780 created "2016-06-24" @default.
- W2052853780 creator A5023233738 @default.
- W2052853780 creator A5049159831 @default.
- W2052853780 date "1986-11-01" @default.
- W2052853780 modified "2023-09-26" @default.
- W2052853780 title "Density-based separation of liposomes by glycerol gradient centrifugation" @default.
- W2052853780 cites W1505126244 @default.
- W2052853780 cites W1513897103 @default.
- W2052853780 cites W1584983848 @default.
- W2052853780 cites W1594934722 @default.
- W2052853780 cites W1984569897 @default.
- W2052853780 cites W1991559715 @default.
- W2052853780 cites W2001825873 @default.
- W2052853780 cites W2008048577 @default.
- W2052853780 cites W2012308391 @default.
- W2052853780 cites W2022335022 @default.
- W2052853780 cites W2025225757 @default.
- W2052853780 cites W2025470299 @default.
- W2052853780 cites W2027185770 @default.
- W2052853780 cites W2028613872 @default.
- W2052853780 cites W2030484073 @default.
- W2052853780 cites W2036452443 @default.
- W2052853780 cites W2045288657 @default.
- W2052853780 cites W2056311643 @default.
- W2052853780 cites W2056615583 @default.
- W2052853780 cites W2060510382 @default.
- W2052853780 cites W2061216302 @default.
- W2052853780 cites W2061357748 @default.
- W2052853780 cites W2089609780 @default.
- W2052853780 cites W2253374107 @default.
- W2052853780 cites W4246432387 @default.
- W2052853780 doi "https://doi.org/10.1016/0003-2697(86)90316-7" @default.
- W2052853780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3812991" @default.
- W2052853780 hasPublicationYear "1986" @default.
- W2052853780 type Work @default.
- W2052853780 sameAs 2052853780 @default.
- W2052853780 citedByCount "25" @default.
- W2052853780 countsByYear W20528537802012 @default.
- W2052853780 countsByYear W20528537802015 @default.
- W2052853780 countsByYear W20528537802016 @default.
- W2052853780 countsByYear W20528537802017 @default.
- W2052853780 countsByYear W20528537802020 @default.
- W2052853780 countsByYear W20528537802021 @default.
- W2052853780 crossrefType "journal-article" @default.
- W2052853780 hasAuthorship W2052853780A5023233738 @default.
- W2052853780 hasAuthorship W2052853780A5049159831 @default.
- W2052853780 hasConcept C111368507 @default.
- W2052853780 hasConcept C113196181 @default.
- W2052853780 hasConcept C127313418 @default.
- W2052853780 hasConcept C185154212 @default.
- W2052853780 hasConcept C185592680 @default.
- W2052853780 hasConcept C2778892434 @default.
- W2052853780 hasConcept C2778918659 @default.
- W2052853780 hasConcept C2780881558 @default.
- W2052853780 hasConcept C41625074 @default.
- W2052853780 hasConcept C43617362 @default.
- W2052853780 hasConcept C55493867 @default.
- W2052853780 hasConcept C97428945 @default.
- W2052853780 hasConceptScore W2052853780C111368507 @default.
- W2052853780 hasConceptScore W2052853780C113196181 @default.
- W2052853780 hasConceptScore W2052853780C127313418 @default.
- W2052853780 hasConceptScore W2052853780C185154212 @default.
- W2052853780 hasConceptScore W2052853780C185592680 @default.
- W2052853780 hasConceptScore W2052853780C2778892434 @default.
- W2052853780 hasConceptScore W2052853780C2778918659 @default.
- W2052853780 hasConceptScore W2052853780C2780881558 @default.
- W2052853780 hasConceptScore W2052853780C41625074 @default.
- W2052853780 hasConceptScore W2052853780C43617362 @default.
- W2052853780 hasConceptScore W2052853780C55493867 @default.
- W2052853780 hasConceptScore W2052853780C97428945 @default.
- W2052853780 hasIssue "1" @default.
- W2052853780 hasLocation W20528537801 @default.
- W2052853780 hasLocation W20528537802 @default.
- W2052853780 hasOpenAccess W2052853780 @default.
- W2052853780 hasPrimaryLocation W20528537801 @default.
- W2052853780 hasRelatedWork W2003731969 @default.
- W2052853780 hasRelatedWork W2009857196 @default.
- W2052853780 hasRelatedWork W2017142036 @default.
- W2052853780 hasRelatedWork W2052853780 @default.
- W2052853780 hasRelatedWork W2080078119 @default.
- W2052853780 hasRelatedWork W2083762140 @default.
- W2052853780 hasRelatedWork W2355592782 @default.
- W2052853780 hasRelatedWork W2411836723 @default.
- W2052853780 hasRelatedWork W2739224825 @default.
- W2052853780 hasRelatedWork W2521696972 @default.
- W2052853780 hasVolume "159" @default.
- W2052853780 isParatext "false" @default.
- W2052853780 isRetracted "false" @default.
- W2052853780 magId "2052853780" @default.
- W2052853780 workType "article" @default.