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• W2052855942 abstract "The proportion of human peripheral blood CD8+ T cells that are CD57+ CD28– is low at birth but increases with age and in individuals infected with human cytomegalovirus (HCMV) or human immunodeficiency virus (HIV). These CD57+ CD28– CD8+ T cells contain large oligoclonal T-cell expansions whose antigen specificity is unknown. We identified clonal expansions of virus-specific memory cytotoxic T-lymphocyte precursors (CTLp) in both healthy carriers of HCMV and in asymptomatic HIV-infected subjects. In each subject, from the T-cell receptor (TCR) β-chain hypervariable sequence of each immunodominant CTL clone, we designed complementary oligonucleotide probes to quantify the size and phenotypic segregation of individual virus-specific CTL clones in highly purified populations of peripheral blood CD8+ T cells. We found large clonal expansions of virus-specific CTL clonotypes in CD57+ CD28– CD8+ T cells. Using limiting dilution analysis, we found functional peptide-specific CTLp at high frequency in CD57+ CD28– cells. Thus, memory CTL specific for persistent viruses account for many oligoclonal expansions within CD57+ CD28– CD8+ T cells." @default.
• W2052855942 created "2016-06-24" @default.
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• W2052855942 date "1999-11-01" @default.
• W2052855942 modified "2023-09-26" @default.
• W2052855942 title "Large clonal expansions of human virus-specific memory cytotoxic T lymphocytes within the CD57⁺ CD28^- CD8⁺T-cell population" @default.
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• W2052855942 doi "https://doi.org/10.1046/j.1365-2567.1999.00901.x" @default.
• W2052855942 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2326947" @default.
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