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• W2052893274 abstract "The electrochemical DNA hybridization sensing of bipolymer polypyrrole and poly(3,4-ethylenedioxythiophene) (PPy–PEDOT) nanotubes functionalized with Ag nanoparticles has been investigated. The bipolymer nanotubes are prepared by simple chemical route and silver nanoparticles (Ag) further deposited over the PPy–PEDOT nanotubes to form PPy–PEDOT–Ag nanocomposite films. DNA labeled at 5′end using 6-mercapto-1-hexhane (HS-ssDNA) is immobilized on the PPy–PEDOT–Ag surface to form PPy–PEDOT–Ag–S-ssDNA and hybridization sensing is done in phosphate buffer. The presence of Ag nanoparticles (~28±5 nm) well dispersed in the polymer composite with high surface area, high electrical conductivity and catalytic activity provides desirable microenvironment for the immobilization of probe DNA with controlled orientation leading to increased hybridization efficiency with target DNA. The morphological and structural characterizations by a scanning electron microscope (SEM) and X-ray diffraction (XRD) confirm the nanotube structure of composite polymer while Raman measurements indicate the efficient interactions between the PPy, PEDOT, Ag and HS-ssDNA. The sensor effectively discriminates different target DNA sequences with PPy–PEDOT–Ag–S-ssDNA substrate. The observed dynamic detection range is found between 1×10 −11 M and 1×10 −14 M with the lowest detection limit (3 σ/b ) of 5.4×10 −15 M. This observed value is of higher sensitivity than that for MWCNT–Ag, PANi–Au, MWCNT–PPy–Au and PPy–PANi–Au composites reported previously. • PPy–PEDOT–AgNP nanocomposite has been synthesized using a simple chemical method. • DNA hybridization sensor based on thiolated ssDNA immobilized on this nanocomposite has been fabricated. • Highly sensitive, stable and reproducible sensor has been achieved. • The developed DNA sensor effectively discriminates between single and double base mismatch target DNA." @default.
• W2052893274 created "2016-06-24" @default.
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• W2052893274 date "2013-09-01" @default.
• W2052893274 modified "2023-09-24" @default.
• W2052893274 title "Polypyrrole–poly(3,4-ethylenedioxythiophene)–Ag (PPy–PEDOT–Ag) nanocomposite films for label-free electrochemical DNA sensing" @default.
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• W2052893274 doi "https://doi.org/10.1016/j.bios.2013.02.049" @default.
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