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• W2052896854 abstract "The aim of this study was to develop a method based on immunomagnetic capture and polymerase chain reaction (IC-PCR assay) for detection of Cryptosporidium parvum and Giardia intestinalis in sewage sludge.The detection limit of the IC-PCR assay for both organisms was 625 oocysts and cysts ml(-1). By hybridization of PCR products the sensitivity could be increased to 125 oocysts and cysts ml(-1). Forty-four sludge samples from 12 wastewater treatment plants were examined. The samples positive for Giardia (9 out of 44) were from eight wastewater plants and the C. parvum genotype 2 samples (3 out of 44) originated from different sewage works.IC-PCR offers the possibility to distinguish between Cryptosporidium and Giardia genotypes. This assay can be used to monitor the presence of these organisms in a community and to determine contamination of sludge used as soil amendment." @default.
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• W2052896854 date "2001-12-01" @default.
• W2052896854 modified "2023-09-27" @default.
• W2052896854 title "Simultaneous detection of Cryptosporidium parvum and Giardia in sewage sludge by IC-PCR" @default.
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• W2052896854 doi "https://doi.org/10.1046/j.1365-2672.2001.01468.x" @default.
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