Matches in SemOpenAlex for { <https://semopenalex.org/work/W2052912120> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2052912120 endingPage "179" @default.
- W2052912120 startingPage "173" @default.
- W2052912120 abstract "Deoxycytidine kinase (dCK) is essential for the phosphorylation of gemcitabine and can predict response to gemcitabine in vivo. Conventional Competitive Template-Reverse Transcriptase-Polymerase Chain Reaction (CT-RT-PCR) was correlated with real time PCR using a Light Cycler (LC) with SYBR-Green detection to enable rapid and sensitive detection of dCK mRNA expression. We used cDNA from human xenografts to establish a relation between dCK activity and gemcitabine sensitivity. A significant correlation of LC-PCR was found with CT-RT-PCR (Pearson: r=0.956; P<0.0001), enzyme activity (Pearson: r=0.972; P=0.003) and gemcitabine sensitivity (Pearson: r=0.695; P=0.048). The LC-PCR was also applied to needle biopsy specimens. In bladder tumors a similar correlation was found, while esophageal tumors with a high dCK expression responded to gemcitabine treatment. The LC is a rapid and reliable method for quantitation of dCK mRNA levels in tumors to predict clinical gemcitabine sensitivity." @default.
- W2052912120 created "2016-06-24" @default.
- W2052912120 creator A5002410223 @default.
- W2052912120 creator A5014845131 @default.
- W2052912120 creator A5021122880 @default.
- W2052912120 creator A5035296183 @default.
- W2052912120 creator A5076275621 @default.
- W2052912120 date "2004-09-01" @default.
- W2052912120 modified "2023-09-26" @default.
- W2052912120 title "Quantitative real time PCR of deoxycytidine kinase mRNA by Light Cycler PCR in relation to enzyme activity and gemcitabine sensitivity" @default.
- W2052912120 cites W1562405218 @default.
- W2052912120 cites W1979266018 @default.
- W2052912120 cites W2039071189 @default.
- W2052912120 cites W2049894527 @default.
- W2052912120 cites W2059868174 @default.
- W2052912120 cites W2092540334 @default.
- W2052912120 cites W2095287544 @default.
- W2052912120 cites W2129793148 @default.
- W2052912120 cites W2135459766 @default.
- W2052912120 cites W2139827791 @default.
- W2052912120 cites W2141937149 @default.
- W2052912120 cites W2313166834 @default.
- W2052912120 cites W4235491236 @default.
- W2052912120 doi "https://doi.org/10.1016/j.canlet.2004.04.016" @default.
- W2052912120 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15327832" @default.
- W2052912120 hasPublicationYear "2004" @default.
- W2052912120 type Work @default.
- W2052912120 sameAs 2052912120 @default.
- W2052912120 citedByCount "21" @default.
- W2052912120 countsByYear W20529121202012 @default.
- W2052912120 countsByYear W20529121202013 @default.
- W2052912120 countsByYear W20529121202018 @default.
- W2052912120 countsByYear W20529121202019 @default.
- W2052912120 crossrefType "journal-article" @default.
- W2052912120 hasAuthorship W2052912120A5002410223 @default.
- W2052912120 hasAuthorship W2052912120A5014845131 @default.
- W2052912120 hasAuthorship W2052912120A5021122880 @default.
- W2052912120 hasAuthorship W2052912120A5035296183 @default.
- W2052912120 hasAuthorship W2052912120A5076275621 @default.
- W2052912120 hasConcept C104317684 @default.
- W2052912120 hasConcept C105580179 @default.
- W2052912120 hasConcept C121608353 @default.
- W2052912120 hasConcept C150903083 @default.
- W2052912120 hasConcept C153911025 @default.
- W2052912120 hasConcept C185592680 @default.
- W2052912120 hasConcept C207001950 @default.
- W2052912120 hasConcept C2777844706 @default.
- W2052912120 hasConcept C2779268695 @default.
- W2052912120 hasConcept C2780258809 @default.
- W2052912120 hasConcept C48023723 @default.
- W2052912120 hasConcept C54355233 @default.
- W2052912120 hasConcept C55493867 @default.
- W2052912120 hasConcept C86803240 @default.
- W2052912120 hasConceptScore W2052912120C104317684 @default.
- W2052912120 hasConceptScore W2052912120C105580179 @default.
- W2052912120 hasConceptScore W2052912120C121608353 @default.
- W2052912120 hasConceptScore W2052912120C150903083 @default.
- W2052912120 hasConceptScore W2052912120C153911025 @default.
- W2052912120 hasConceptScore W2052912120C185592680 @default.
- W2052912120 hasConceptScore W2052912120C207001950 @default.
- W2052912120 hasConceptScore W2052912120C2777844706 @default.
- W2052912120 hasConceptScore W2052912120C2779268695 @default.
- W2052912120 hasConceptScore W2052912120C2780258809 @default.
- W2052912120 hasConceptScore W2052912120C48023723 @default.
- W2052912120 hasConceptScore W2052912120C54355233 @default.
- W2052912120 hasConceptScore W2052912120C55493867 @default.
- W2052912120 hasConceptScore W2052912120C86803240 @default.
- W2052912120 hasIssue "2" @default.
- W2052912120 hasLocation W20529121201 @default.
- W2052912120 hasLocation W20529121202 @default.
- W2052912120 hasOpenAccess W2052912120 @default.
- W2052912120 hasPrimaryLocation W20529121201 @default.
- W2052912120 hasRelatedWork W1930516194 @default.
- W2052912120 hasRelatedWork W1974995659 @default.
- W2052912120 hasRelatedWork W2004348534 @default.
- W2052912120 hasRelatedWork W2016472552 @default.
- W2052912120 hasRelatedWork W2052912120 @default.
- W2052912120 hasRelatedWork W2067769249 @default.
- W2052912120 hasRelatedWork W2118512375 @default.
- W2052912120 hasRelatedWork W2132958436 @default.
- W2052912120 hasRelatedWork W3149052091 @default.
- W2052912120 hasRelatedWork W4361836217 @default.
- W2052912120 hasVolume "213" @default.
- W2052912120 isParatext "false" @default.
- W2052912120 isRetracted "false" @default.
- W2052912120 magId "2052912120" @default.
- W2052912120 workType "article" @default.