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• W2052924755 abstract "Chemical analysis of adult females of Onchocerca gibsoni gave estimated chitin contents of 200-500 micrograms (g dry weight)-1. Egg shells from both O. gibsoni and Onchocerca volvulus stained with Calcofluor white and with fluorescent wheat germ agglutinin as shown by fluorescent light microscopy, and bound gold-labelled wheat germ agglutinin as shown by electron microscopy, under conditions specific for chitin. The egg shells appeared as single electron dense layers from 50 to 85 nm in thickness. Purified chitinase digested these egg shells, leaving coiled microfilariae unattacked. We conclude that chitin is a major component of the egg shells." @default.
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• W2052924755 date "1987-10-01" @default.
• W2052924755 modified "2023-09-26" @default.
• W2052924755 title "Chitin in egg shells of Onchocerca gibsoni and Onchocerca volvulus" @default.
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• W2052924755 doi "https://doi.org/10.1016/0166-6851(87)90090-9" @default.
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