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• W2052964446 abstract "DNA preparations from 25 eukaryotes, ranging from yeast to man, were analyzed in their base composition, sedimentation coefficient, modal and mean buoyant density in CsCl. Four mammalian and two amphibian DNAs were fractionated by preparative Cs 2 SO 4 −Ag + density gradient centrifugation. The CsCl band profile of each fraction was analyzed in terms of Gaussian curves; this allowed the buoyant densities and the relative amounts of the components present in each DNA to be assessed. Direct analysis of the CsCl band profiles of unfractionated DNA showed a satisfactory agreement with the results obtained in the combined Cs 2 SO 4 −Ag + , CsCl investigations. This simpler procedure was therefore applied to all 25 eukaryotic DNAs. The main finding of this work is the recognition of phylogenetic differences at the macromolecular level in the organization of eukaryotic genomes. The differences concerning the main band DNA are: (a) the three major components (1·697, 1·704 and 1·709 g/cm 3 ) first observed in the main band of calf DNA by Filipski et al. (1973) were also found in the other ten mammalian DNAs investigated here; similar components appear to be present in avian genomes; in both cases, the 1·704 and 1·709 g/cm 3 components are responsible for the skewness to the heavy side of the CsCl main band of these DNAs; (b) the DNAs of reptiles, amphibians and fish showed a much lower and decreasing skewness of their CsCl bands compared to both mammalian and avian DNAs; the Cs 2 SO 4 −Ag + , CsCl combined analysis of two amphibian DNAs revealed discrete components, different from those of mammals and birds; (c) essentially symmetrical bands in CsCl were exhibited by three invertebrate DNAs; (d) the DNAs from three unicellular eukaryotes exhibited perfectly symmetrical bands in CsCl and could not be resolved into discrete comoponents. A number of observations on minor and satellite DNA components collected in this work are reported." @default.
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• W2052964446 date "1976-11-01" @default.
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• W2052964446 title "An analysis of eukaryotic genomes by density gradient centrifugation" @default.
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• W2052964446 doi "https://doi.org/10.1016/s0022-2836(76)80104-0" @default.
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