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- W2052999004 abstract "In diluted blood predominantly T cells responded to purified PHA (pure leucoagglutinin Wellcome). Red cells and non-T mononuclear cells increased PHA responsiveness of T cells at concentrations present in the peripheral blood. Addition of polymorphonuclear leucocytes was a very slight further advantage. The incorporation of [3H]thymidine reflected the numbers of T cells in culture, although not perfectly linearly, since counts increased when more T cells were added to the cultures. It was therefore concluded that in cultures of diluted whole blood non-specific amplification mechanisms were provided and responses reflected the performance of stimulated T lymphocytes. Since the method is simple and the number of technical variables is relatively small, it is possible to establish a ‘normal’ range of PHA responsiveness expressed in absolute counts per minute, and thus directly compare results from different laboratories." @default.
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- W2052999004 title "Lymphocyte activation. VIII. The application of a whole blood test to the qauntitative analysis of PHA responsive T cells" @default.
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- W2052999004 doi "https://doi.org/10.1016/0022-1759(76)90003-x" @default.
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