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• W2053001517 abstract "Article31 May 2007free access Truncations of amphiphysin I by calpain inhibit vesicle endocytosis during neural hyperexcitation Yumei Wu Yumei Wu Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Shuang Liang Shuang Liang Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Yoshiya Oda Yoshiya Oda Laboratory of Seed Finding Technology, Eisai Co., Ltd, Tsukuba, Japan Search for more papers by this author Iori Ohmori Iori Ohmori Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Tei-ichi Nishiki Tei-ichi Nishiki Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Kohji Takei Kohji Takei Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Hideki Matsui Hideki Matsui Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Kazuhito Tomizawa Corresponding Author Kazuhito Tomizawa Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Yumei Wu Yumei Wu Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Shuang Liang Shuang Liang Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Yoshiya Oda Yoshiya Oda Laboratory of Seed Finding Technology, Eisai Co., Ltd, Tsukuba, Japan Search for more papers by this author Iori Ohmori Iori Ohmori Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Tei-ichi Nishiki Tei-ichi Nishiki Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Kohji Takei Kohji Takei Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Hideki Matsui Hideki Matsui Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Kazuhito Tomizawa Corresponding Author Kazuhito Tomizawa Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Search for more papers by this author Author Information Yumei Wu1, Shuang Liang2, Yoshiya Oda3, Iori Ohmori1, Tei-ichi Nishiki1, Kohji Takei2, Hideki Matsui1 and Kazuhito Tomizawa 1 1Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan 2Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan 3Laboratory of Seed Finding Technology, Eisai Co., Ltd, Tsukuba, Japan *Corresponding author. Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Shikata-cho 2-5-1, Okayama 700-8558, Japan. Tel.: +81 86 235 7107; Fax: +81 86 235 7111; E-mail: [email protected] The EMBO Journal (2007)26:2981-2990https://doi.org/10.1038/sj.emboj.7601741 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Under normal physiological conditions, synaptic vesicle endocytosis is regulated by phosphorylation and Ca2+-dependent dephosphorylation of endocytic proteins such as amphiphysin and dynamin. To investigate the regulatory mechanisms that may occur under the conditions of excessive presynaptic Ca2+ influx observed preceding neural hyperexcitation, we examined hippocampal slices following high-potassium or high-frequency electrical stimulation (HFS). In both cases, three truncated forms of amphiphysin I resulted from cleavage by the protease calpain. In vitro, the binding of truncated amphiphysin I to dynamin I and copolymerization into rings with dynamin I were inhibited, but its interaction with liposomes was not affected. Moreover, overexpression of the truncated form of amphiphysin I inhibited endocytosis of transferrin and synaptic vesicles. Inhibiting calpain prevented HFS-induced depression of presynaptic transmission. Finally, calpain-dependent amphiphysin I cleavage attenuated kainate-induced seizures. These results suggest that calpain-dependent cleavage of amphiphysin I inhibits synaptic vesicle endocytosis during neural hyperexcitation and demonstrate a novel post-translational regulation of endocytosis. Introduction Clathrin-mediated endocytosis is the major mechanism for maintaining the synaptic vesicle recycling pool for synaptic transmission in small central synapses during repetitive high-frequency electrical stimulation (HFS) or even physiological stimulation (Granseth et al, 2006). Clathrin-mediated endocytosis consists of four steps: nucleation, invagination, fission and uncoating. Many endocytosis-related proteins are recruited to form complexes on the retrieved pits at each step after calcium-dependent calcineurin activity is activated by neural excitation. Amphiphysin I, a major dynamin-binding partner localized on the collar of retrieved vesicles, plays a key role in clathrin-mediated endocytosis of synaptic vesicles (Wigge and McMahon, 1998; Zhang and Zelhof, 2002; Evergren et al, 2004). Amphiphysin I mediates invagination and fission of synaptic vesicles, senses and facilitates membrane curvature via its BAR domain (Zhang and Zelhof, 2002; Peter et al, 2004) and stimulates the GTPase activity of dynamin in the presence of lipid membrane (Yoshida et al, 2004; Yoshida and Takei, 2005). The function of both amphiphysin I and dynamin is known to be regulated by phosphorylation and Ca2+-dependent dephosphorylation (Slepnev et al, 1998; Cousin and Robinson, 2001; Tan et al, 2003; Tomizawa et al, 2003) under physiological conditions. Under resting conditions, endocytosis proteins such as amphiphysin I and dynamin are phosphorylated by Cdk5 (cyclin-dependent kinase 5). Once neural excitability is transferred to the nerve terminal, calcium influx to the presynaptic terminal occurs through VDCCs and activates phosphatase 2B calcineurin, which dephosphorylates the endocytic proteins. These proteins then form a complex and induce clathrin-mediated endocytosis. Massive amount of Ca2+ enter presynaptic terminals during the neural hyperexcitation observed in neurodegenerative disorders such as ischemia/anoxia, epilepsy, stroke, trauma and Alzheimer's disease. Previous studies have shown that hyperexcitation, such as repetitive HFS or high potassium stimulation, at presynapses induces short-term depression at postsynapses in hippocampal neurons (Stevens and Wesseling, 1999; Sara et al, 2002). This short-term depression is thought to be a neuroprotective mechanism for protecting neurons from hyperexcitability. However, the mechanism of the short-term depression after Ca2+ influx is unclear. Previous studies have shown that synaptic vesicle endocytosis, which contributes to maintaining synaptic transmission, is inhibited during synaptic depression (Sun et al, 2002; Wu, 2004). These results suggest that inhibition of clathrin-mediated endocytosis may be involved in the short-term depression at mammalian synapses after excessive presynaptic Ca2+ influx. Calcium-dependent calpain activity is increased by massive calcium influx, and promotes the degradation of key cytoskeletal and membrane proteins or cleavage of many other important proteins such as calcineurin in neuroexcitotoxicity (Wu et al, 2004; Czogalla and Sikorski, 2005), which has been shown to be related to various neurodegenerative disorders. Moreover, several presynaptic proteins such as spectrin (Saido et al, 1993), PKC (Sessoms et al, 1992) and p35 (Lee et al, 2000), a Cdk5 activator, are substrates of calpain. The function of these proteins may be affected by calpain cleavage. In the present study, we found that the important endocytosis protein amphiphysin I is cleaved by calpain during high potassium stimulation, repetitive high-frequency stimulation and kainate (KA)-induced seizures. Calpain-cleaved amphiphysin I lost its function of binding with dynamin in vitro, and overexpression of the truncated form of amphiphysin I in COS-7 cells and neurons inhibited clathrin-mediated endocytosis. Inhibition of clathrin-mediated endocytosis of synaptic vesicles by calpain-dependent amphiphysin I cleavage is involved in repetitive HFS-induced synaptic depression and KA-induced seizures. Results High K+ induces multiple amphiphysin I cleavages by calpain in mouse hippocampal slices We first investigated whether amphiphysin I was cleaved under conditions of excessive Ca2+ influx into nerve terminals (80 mM KCl) or under neurotoxic conditions (500 μM L-glutamate) in the hippocampal slices. In both cases, three major truncated products of amphiphysin I were detected by Western blotting analysis using anti-amphiphysin I antibodies recognizing the N terminus (Figure 1A). The antibodies reacted with 52, 47 and 43-kDa proteins on SDS–PAGE gels. The induction of these truncations was greater with high potassium (high K+) stimulation than with glutamate stimulation (Figure 1A). Moreover, a weak fourth band of amphiphysin I was sometimes detected in the hippocampal slices given high K+ stimulation (arrow in Figure 1A). These results suggest that excessive Ca2+ influx in presynaptic terminus may induce the cleavage of amphiphysin I. The Ca2+-dependent cysteine protease calpain is involved in various neurological processes, and an increase in calpain activity is related to the pathophysiology of neurodegenerative disorders such as neuroexcitotoxicity and ischemia (Rami, 2003; Wu et al, 2004). We therefore examined whether calpain is involved in the cleavage of amphiphysin I after high K+ stimulation. Figure 1B shows the time-dependent cleavages of amphiphysin I and α-spectrin, a physiological substrate of calpain, in hippocampal slices after high K+ stimulation. Amphiphysin I was rapidly cleaved with obvious truncations observed 30 s after the stimulation (Figure 1B), and 41% of amphiphysin I was cleaved 20 min after the stimulation. α-Spectrin was also rapidly cleaved after high K+ stimulation and was more extensively cleaved than amphiphysin I after 20 min of stimulation (Figure 1B). Subcellular fractionation showed that two isoforms of calpain, μ- and m-calpain, existed in the membrane and cytosol fractions of purified synaptosomes (LP2 and LS2 fractions in Figure 1C). Two potent calpain inhibitors, ALLM and ALLN, inhibited the high K+-induced truncations of amphiphysin I in the hippocampal slices (Figure 1D). Moreover, m-calpain cleaved recombinant amphiphysin I in vitro, yielding three truncated forms with molecular masses equal to those of the truncated forms observed in hippocampal slices after high K+ stimulation (Figure 1E). A high concentration (40 nM) of m-calpain almost completely cleaved amphiphysin I to the 43-kDa form, and 52-kDa amphiphysin I was not observed (Figure 1E). We also examined the effect of μ-calpain on amphiphysin I cleavage in vitro (Supplementary Figure 1). μ-Calpain cleaved amphiphysin I to the same three major truncated forms as m-calpain, and in addition to one new truncated form between the 52 and 47-kDa forms. These results suggest that amphiphysin I may be cleaved by calpain during neural hyperexcitation. Figure 1.High K+ induces multiple amphiphysin I (Amphi. I) cleavages by calpain in mouse hippocampal slices. (A) Western blotting analysis of hippocampal slices stimulated with L-glutamate or high K+ and then allowed to rest for the indicated times. Probing with anti-amphiphysin I antibody recognizing the N terminus demonstrated the presence of three truncated forms of amphiphysin I. Arrow, fourth truncated form of amphiphysin I. (B) Time-dependent cleavages of amphiphysin I and α-spectrin after high K+ stimulation of hippocampal slices for the indicated times. The lowest panel shows the quantitative analysis of the expression changes of FL amphiphysin I and α-spectrin after high K+ stimulation. The expression levels of both proteins of the control (time=0 min) were set at 1. (C) Subcellular fractionation of μ- and m-calpain from cerebral cortex as shown by probing with anti-μ- and m-calpain antibodies. Anti-synaptotagmin antibodies were used to monitor synaptosomal fractions. H, total homogenate; P2, crude synaptosomal pellet; LP2, membrane fraction of purified synaptosomes; LS2, cytosol fraction of purified synaptosomes. (D) Anti-amphiphysin I immunoblotting showing that calpain inhibitors, ALLM (AM) or ALLN (AN), blocked high K+-induced amphiphysin I cleavage. (E) Anti-amphiphysin I immunoblotting showing the in vitro cleavage of recombinant amphiphysin I (0.5 μg/μl) by various concentrations of recombinant m-calpain. Download figure Download PowerPoint Identification of the cleavage sites of amphiphysin I by calpain To determine whether calpain cleaved the N- or C terminus of amphiphysin I, two different anti-amphiphysin I antibodies (designated antibody I and II), which recognized the N- and the C terminus, respectively, were used to detect the truncations in vitro (Figure 2A). Antibody I detected three truncated forms (Figure 2A). In contrast, antibody II detected a truncated form of amphiphysin I with molecular mass of 76 kDa. CBB staining showed three truncations of amphiphysin I and the molecular masses of the truncations on SDS–PAGE agreed with those of amphiphysin I truncations detected with antibody I. However, obvious truncations of amphiphysin I corresponding to the 76-kDa form was not observed on CBB-stained gels. Moreover, antibody II did not detect any truncated forms in the hippocampal slices after high K+ stimulation (Figure 2B). These results suggest that the truncated forms of amphiphysin I are N-terminal fragments, and C-terminal fragments may be degraded after high K+ stimulation. The precise cleavage sites of amphiphysin I were identified by mass spectrometric analysis (MS). Interestingly, MS showed that the protein was cleaved at nine sites of the C terminus (positions 333, 377, 392, 454, 478, 527, 531, 593 and 609) (Figure 2C and Supplementary Figures 2 and 3). Figure 2C shows a comparison of the molecular weights of the truncated forms of amphiphysin I cleaved in vitro by calpain and the recombinant proteins of each truncated form (1–333, 1–377 and 1–392) identified by MS. The molecular weights of the cleaved forms of amphiphysin I agreed with those of the recombinant proteins. These results suggest that positions 333, 377 and 392 correspond to the truncation sites after high K+ stimulation among the cleavage sites identified by MS. Figure 2.Sites of cleavage of amphiphysin I by calpain. (A) Upper panel, schematic diagram of human amphiphysin I and the recognition sites of each anti-amphiphysin I antibody (recognizing the N terminus (I) or C terminus (II)). Lower panel, Western blotting showing the m-calpain (7 nM)-induced truncation products of recombinant amphiphysin I recognized by anti-amphiphysin I antibodies I and II. CBB staining showing amphiphysin I was mainly cleaved to three fragments corresponding to the truncated products detected by antibody I. (B) No truncated form of amphiphysin I was detected by antibody II in high K+-treated hippocampal slices. (C) Three major truncations of amphiphysin I. Upper panel, scheme of the cleavage sites by m-calpain identified by MS analysis. Lower panel, comparison of the molecular weights of amphiphysin I cleaved in vitro by m-calpain with those of the recombinant proteins of each truncation form identified by MS. Download figure Download PowerPoint Interaction of amphiphysin I with liposomes and dynamin I inhibits the cleavage by calpain Amphiphysin I is associated with synaptic plasma membrane through the BAR domain (Zhang and Zelhof, 2002; Peter et al, 2004). This phenomenon can be reconstituted as a tubulation of liposomes in a cell-free system (Figure 3A). Moreover, the protein binds dynamin I via the SH3 domain and coassembles with dynamin I into rings either in solution or on lipid tubules (Figure 3A). We examined whether the association of amphiphysin I with liposomes and/or dynamin I affected the truncations by calpain. Amphiphysin alone was completely cleaved to three truncated forms by calpain (Figure 3B, lane 5). In contrast, amphiphysin I bound to liposomes was resistant to cleavage by calpain regardless of the binding with dynamin I (Figure 3B, lanes 2 and 3). Interaction with dynamin I inhibited the production of two of the truncated forms of amphiphysin I with molecular masses of 47 and 43 kDa (Figure 3B, lane 6). On the other hand, dynamin I was not cleaved by calpain under various conditions (Figure 3C). To investigate whether liposomes influenced calpain activity, calpain cleavage of calcineurin, a good substrate of calpain (Wu et al, 2004), was examined in the presence of liposomes. Calpain cleaved calcineurin as efficiently in the presence of liposomes as in their absence, suggesting that liposomes have no effect on calpain activity (Figure 3D). These results suggest that the association of amphiphysin I with synaptic membranes and dynamin may be crucial for the regulation of its cleavage by calpain. The interaction of amphiphysin I with dynamin I is strictly regulated by phosphorylation and dephosphorylation (Tomizawa et al, 2003; Liang et al, 2007). Phosphorylation of amphiphysin I by Cdk5 inhibits the interaction with endocytic proteins, whereas calcineurin-dependent dephosphorylation of amphiphysin I induces this interaction (Tomizawa et al, 2003). It was next examined whether calpain-dependent cleavage of amphiphysin I was regulated by phosphorylation and dephosphorylation. A phospho-mimic mutant of amphiphysin I (5D) in which five sites (S261, S272, S276, S285 and T310) phosphorylated by Cdk5 were replaced with Asp was cleaved by m-calpain the same way as dephospho-amphiphysin I in vitro (Figure 3E). However, preincubation with FK506, a potent calcineurin inhibitor, increased the levels of high K+-stimulated amphiphysin I cleavages in hippocampal slices compared with roscovitine, a potent Cdk5 inhibitor (Figure 3F). These results suggest that phosphorylation of amphiphysin I does not directly influence the cleavage of amphiphysin I by calpain, but that the phosphorylation inhibits the formation of a complex with endocytic proteins and synaptic vesicle membranes, resulting in the induction of cleavage of amphiphysin I by calpain during hyperexcitation. Figure 3.Interaction of amphiphysin I (Amphi. I) with liposomes and dynamin I (Dyn. I) inhibits the cleavage by calpain. (A) Morphological analysis of the interaction of amphiphysin I with liposomes and dynamin I at the electron microscopic level. Amphiphysin I recruits dynamin I onto liposomes, forming collars on tubulated liposomes. Bars in upper panels, 2 μm, bar in lower panel, 100 nm. (B) Effects of the interaction of amphiphysin I with liposomes and dynamin I on its cleavage by calpain are shown by anti-amphiphysin I immunoblotting of liposome and liposome-free fractions of the incubation mixture. (C) Anti-dynamin I immunoblotting shows that dynamin I was not cleaved by calpain in the presence or absence of either liposomes or amphiphysin I. (D) The effect of liposomes on calpain activity was examined by incubating purified calcineurin in the presence or absence of liposomes. Calcineurin cleavage by calpain was examined by Western blotting with anti-calcineurin antibodies. (E) Comparison of calpain-dependent cleavage between WT amphiphysin I and its phospho-mimic mutant form in vitro. For the mutant, Cdk5 phosphorylation sites (S261, S272, S276, S285 and T310) of amphiphysin I were replaced with Asp (5D). Both types of recombinant amphiphysin I were incubated with 2 nM m-calpain for 30 min. (F) The effect of phosphorylation of amphiphysin I on its cleavage by calpain was examined in hippocampal slices treated with either FK506 (FK), an inhibitor of calcineurin, or roscovitine (Ros.), a Cdk5 inhibitor. Hippocampal slices were preincubated with and without 50 μM FK or 10 μM Ros. for 30 min at 30°C and were then treated with 80 mM KCl for 15 min. Homogenized slices were analyzed by immunoblotting with anti-amphiphysin I and anti-synaptotagmin antibodies. Download figure Download PowerPoint Amphiphysin I cleaved by calpain and a truncated form (1–392) are capable of tubulation with liposomes, but do not form ring structures with dynamin I To investigate whether calpain-dependent cleavage of amphiphysin I affects its function, the interactions of amphiphysin I with liposomes and dynamin I were examined in vitro (Figure 4). Liposomes formed tubules with cleaved amphiphysin I (Figure 4Ac) just as they did with full-length (FL) amphiphysin I (Figure 4Aa). Recombinant protein corresponding to one of the truncated forms (1–392) also underwent tubule formation with liposomes (Figure 4Ad). These results suggest that amphiphysin I cleaved by calpain may be capable of binding to synaptic membranes. On the other hand, amphiphysin I cleaved by calpain (Figure 4Ag) or the recombinant protein corresponding to the truncated form (Figure 4Ah) did not form ring structures with dynamin I, whereas FL amphiphysin I did form small rings (Figure 4Ae and f). Moreover, when incubated with liposomes and dynamin I, the truncated form failed to form the thick helical collars on tubules (Figure 4Aj), which are formed with FL amphiphysin I (Figure 4Ai). These results indicate that calpain-dependent truncation of amphiphysin I inhibits the interaction with dynamin I. Since both dynamin ring structures and thick helical collars on tubules are required for the formation of endocytic synaptic vesicles, our findings suggest that both of these processes are inhibited following calpain cleavage of amphiphysin I during hyperexcitation. Figure 4.Amphiphysin I cleaved by calpain and a truncated form (1–392) are capable of tubulation with liposomes, but do not form ring structures with dynamin I (A: a–j), and inhibitory effect of calpain-dependent truncated form of amphiphysin I on transferrin uptake in Cos-7 cells (B) and FM 4–64 labeling in primary culture of hippocampal neurons (C). (A: a–d) The effect of truncation on the ability of amphiphysin I to form tubules with liposomes. As a chelator of Ca2+, EGTA inhibits calpain activity. (A: e–h) The effect of both forms of amphiphysin I on the formation of ring structures with dynamin I. (A: i, j) Comparison of helical collars on the tubules of FL and truncated amphiphysin I when incubated with dynamin I plus liposomes. Bars in A: d and h, 500 nm; bars in A: i and j, 100 nm. (B) Confocal laser scanning micrographs of: left panel, EGFP expression on COS-7 cells transfected with pIRES2-EGFP vector only (Vector), the vector containing wild-type amphiphysin I cDNA (WT) and the vector containing truncated amphiphysin I (1–392) cDNA (truncated form); middle panel, transferrin (Tf.) uptake in similarly transfected cells exposed to transferrin 24 h later; and, right panel, showing the overlay of EGFP and transferrin uptake. Transferrin uptake was inhibited in cells transfected with truncated amphiphysin I (arrows); bar, 10 μm. For quantification of the results, transferrin uptake was calculated as the mean fluorescence density for 315–660 cells under each condition and the mean values (±s.e.m.) were expressed as a percentage of normal transferrin uptake in untransfected cells. *P<0.01. (C) Fluorescence imaging micrographs of: left panel, EGFP expression on presynapses of neurons transfected with vector, WT amphiphysin I and the truncated form (amphi. 1–392); middle panel, subtracted images of FM 4–64 (loading minus unloading); right panel showing overlay of EGFP expression and FM 4–64 labeling. Bar, 10 μm. FM 4–64 labeling was inhibited in boutons transfected with truncated amphiphysin I (arrows). Right graph shows the results of quantitative analysis. FM 4–64 labeling was calculated as the integral fluorescence intensity for 47–55 boutons under each condition and the mean values (±s.e.m.) were expressed as a percentage of normal FM 4–64 labeling in untransfected boutons. *P<0.01. Download figure Download PowerPoint We further investigated whether calpain-dependent cleavage of amphiphysin I influenced clathrin-mediated endocytosis in cells. Wild-type (WT) amphiphysin I and one of its truncated forms (1–392) were overexpressed in Cos-7 cells, and transferrin uptake in the cells was examined (Figure 4B). Transferrin uptake in the truncated amphiphysin I-overexpressing cells was inhibited by 61% compared to that in WT amphiphysin I-overexpressing cells (Figure 4B). Moreover, the results were confirmed in rat hippocampal neurons. We investigated whether calpain-dependent cleavage of amphiphysin I affected presynaptic vesicle endocytosis in rat hippocampal neurons. FM 4–64 labeling in the truncated amphiphysin I-overexpressing boutons was inhibited by 57% compared to that in WT amphiphysin I-overexpressing boutons. These results indicate that calpain-dependent cleavage of amphiphysin I inhibits clathrin-mediated synaptic vesicle endocytosis. Amphiphysin I cleavage by calpain inhibits excessive synaptic transmission in hippocampal slices and in vivo Repetitive, high-frequency electrical nerve stimulation (e.g., 10 Hz) causes short-term synaptic depression in hippocampal neurons and neuromuscular junctions (Figure 5C and Wu and Betz, 1998) mainly because synaptic vesicles are depleted (Wang and Kaczmarek, 1998). Clathrin-mediated endocytosis is inhibited during frequency-dependent depression, resulting in the depletion of the recycling pool of synaptic vesicles (Wu and Betz, 1998; Wu, 2004). These data along with the present results led us to hypothesize that amphiphysin I is cleaved by calpain during HFS, and that the cleavage causes high frequency-induced depression of synaptic transmission through the inhibition of clathrin-mediated endocytosis. To test this hypothesis, we examined whether calpain-dependent cleavage of amphiphysin I was involved in the depression of the field EPSP (fEPSP) at the synapses from Schaffer collaterals onto CA1 pyramidal cells in hippocampal slices. HFS (10 Hz) induced amphiphysin I cleavage, whereas low-frequency stimulation (LFS, 0.03 Hz) produced no cleavage (Figure 5A). Blocking calpain with ALLM inhibited the HFS-induced cleavages. Thus, calpain cleaves amphiphysin I during repetitive HFS in hippocampal slices. Perfusion of ALLM for 30 min had no effect on fEPSP slope when slices were stimulated with LFS (Figure 5B). In contrast, ALLM inhibited HFS-induced fEPSP depression (Figure 5C and D). To clarify whether the effect of calpain inhibition on the fEPSP depression is exerted in the presynapse or in the postsynapse, the effect of ALLM on paired-pulse facilitation (PPF) was examined in hippocampal slices. PPF is a transient form of presynaptic plasticity, in which the second of two closely spaced stimuli elicits enhanced transmitter release, because of residual calcium ions in the presynaptic terminal after the first stimulus (Zucker, 1989). PPF at 100 and 150 ms interpulse intervals was decreased after HFS (10 Hz) compared with that after LFS (Figure 5E). In the ALLM-treated slices, in contrast, PPF after HFS was not decreased compared with that after LFS (Figure 5E). These results suggest that cleavages of amphiphysin I by calpain may partially account for the HFS-induced depression of synaptic transmission through the inhibition of clathrin-mediated endocytosis of synaptic vesicles. Figure 5.Amphiphysin I cleavage by calpain inhibits excessive synaptic transmission in hippocampal slices and in vivo. (A) Anti-amphiphysin I immunoblot showing the effects of either no electrical stimulation (C), LFS (L, 0.03 Hz) or HFS (H, 10 Hz) on calpain-dependent amphiphysin I cleavage in mouse hippocampal slices, without or with ALLM preincubation. (B) ALLM inhibition of calpain had no effect on fEPSP slope during LFS. (n=7) (C) Effect of calpain inhibition (ALLM) on fEPSP depression induced by HFS. Control, n=8; ALLM, n=11. (D) Comparison of fEPSP slopes at 15 min after LFS and HFS in the ALLM-perfused slices and controls. *P<0.05. (E) PPF in hippocampal slices following LFS is reduced following HFS, an effect that is blocked by inhibiting calpain with ALLM. ALLM had no effect on PPF following LFS. The data represent the ratios of the second EPSP slopes versus the first fEPSP slopes separated by the given intervals. As a control, hippocampal slices were incubated with DMSO instead of ALLM. *P<0.05, **P<0.01 compared with Cont. n=10–15. (F) Anti-amphiphysin I immunoblot showing the ability of KA injections to induce calpain-dependent amphiphysin I cleavage in the hippocampus of FVB/NJ mice. Preincubation wit" @default.
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• W2053001517 title "Truncations of amphiphysin I by calpain inhibit vesicle endocytosis during neural hyperexcitation" @default.
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