Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053018237> ?p ?o ?g. }
- W2053018237 endingPage "2869" @default.
- W2053018237 startingPage "2869" @default.
- W2053018237 abstract "Hepatitis B is a worldwide public health problem. To explore the feasibility of hepatitis B virus (HBV) vertical transmission via oocytes, the presence and integration of HBV DNA in mouse oocytes were studied.Genomic DNA was isolated and metaphases were prepared, respectively from mouse oocytes cocultured with pBR322-HBV DNA plasmids. PCR, Southern blot, dot hybridization and fluorescence in situ hybridization (FISH) were performed to explore the existence and integration of HBV DNA in oocytes.PCR detected positive bands in the tested samples, and then Southern blot revealed clear hybridization signals in PCR products. Final washing solutions were collected for dot hybridization and no signal for HBV DNA was observed, which excluded the possibility that contamination of washing solutions gave rise to positive results of PCR and Southern blot. FISH demonstrated that 36 of 1,000 metaphases presented positive signals.HBV DNA sequences are able to pass through the zona and oolemma to enter into oocytes and to integrate into their chromosomes. HBV DNA sequences might be brought into embryo via oocytes as vectors when they are fertilized with normal spermatozoa." @default.
- W2053018237 created "2016-06-24" @default.
- W2053018237 creator A5040258718 @default.
- W2053018237 date "2005-01-01" @default.
- W2053018237 modified "2023-09-27" @default.
- W2053018237 title "Presence and integration of HBV DNA in mouse oocytes" @default.
- W2053018237 cites W181244740 @default.
- W2053018237 cites W192583765 @default.
- W2053018237 cites W193057416 @default.
- W2053018237 cites W1969836219 @default.
- W2053018237 cites W1971031166 @default.
- W2053018237 cites W1978333655 @default.
- W2053018237 cites W1982146044 @default.
- W2053018237 cites W1985439784 @default.
- W2053018237 cites W1986973051 @default.
- W2053018237 cites W1987499970 @default.
- W2053018237 cites W1988570524 @default.
- W2053018237 cites W1993171642 @default.
- W2053018237 cites W2007380054 @default.
- W2053018237 cites W2009078075 @default.
- W2053018237 cites W2010024453 @default.
- W2053018237 cites W2011727762 @default.
- W2053018237 cites W2013054809 @default.
- W2053018237 cites W2016279068 @default.
- W2053018237 cites W2043335783 @default.
- W2053018237 cites W2052664378 @default.
- W2053018237 cites W2054464808 @default.
- W2053018237 cites W2054565628 @default.
- W2053018237 cites W2063727644 @default.
- W2053018237 cites W2064457663 @default.
- W2053018237 cites W2066672332 @default.
- W2053018237 cites W2071866772 @default.
- W2053018237 cites W2075155718 @default.
- W2053018237 cites W2076171352 @default.
- W2053018237 cites W2086990267 @default.
- W2053018237 cites W2088249412 @default.
- W2053018237 cites W2088800638 @default.
- W2053018237 cites W2088904193 @default.
- W2053018237 cites W2126860077 @default.
- W2053018237 cites W2136011659 @default.
- W2053018237 cites W2147155982 @default.
- W2053018237 cites W2159146123 @default.
- W2053018237 cites W2166023204 @default.
- W2053018237 cites W2338800987 @default.
- W2053018237 cites W2372870958 @default.
- W2053018237 cites W2429206005 @default.
- W2053018237 cites W2434718057 @default.
- W2053018237 cites W2460919693 @default.
- W2053018237 cites W4238513495 @default.
- W2053018237 cites W68316507 @default.
- W2053018237 doi "https://doi.org/10.3748/wjg.v11.i19.2869" @default.
- W2053018237 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4305652" @default.
- W2053018237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15902721" @default.
- W2053018237 hasPublicationYear "2005" @default.
- W2053018237 type Work @default.
- W2053018237 sameAs 2053018237 @default.
- W2053018237 citedByCount "20" @default.
- W2053018237 countsByYear W20530182372012 @default.
- W2053018237 countsByYear W20530182372013 @default.
- W2053018237 countsByYear W20530182372014 @default.
- W2053018237 countsByYear W20530182372016 @default.
- W2053018237 countsByYear W20530182372020 @default.
- W2053018237 countsByYear W20530182372022 @default.
- W2053018237 countsByYear W20530182372023 @default.
- W2053018237 crossrefType "journal-article" @default.
- W2053018237 hasAuthorship W2053018237A5040258718 @default.
- W2053018237 hasBestOaLocation W20530182371 @default.
- W2053018237 hasConcept C104317684 @default.
- W2053018237 hasConcept C105580179 @default.
- W2053018237 hasConcept C130840561 @default.
- W2053018237 hasConcept C133307689 @default.
- W2053018237 hasConcept C153911025 @default.
- W2053018237 hasConcept C159047783 @default.
- W2053018237 hasConcept C17757408 @default.
- W2053018237 hasConcept C22744801 @default.
- W2053018237 hasConcept C2522874641 @default.
- W2053018237 hasConcept C2777542201 @default.
- W2053018237 hasConcept C2780593183 @default.
- W2053018237 hasConcept C30481170 @default.
- W2053018237 hasConcept C49105822 @default.
- W2053018237 hasConcept C54355233 @default.
- W2053018237 hasConcept C552990157 @default.
- W2053018237 hasConcept C81439078 @default.
- W2053018237 hasConcept C86803240 @default.
- W2053018237 hasConceptScore W2053018237C104317684 @default.
- W2053018237 hasConceptScore W2053018237C105580179 @default.
- W2053018237 hasConceptScore W2053018237C130840561 @default.
- W2053018237 hasConceptScore W2053018237C133307689 @default.
- W2053018237 hasConceptScore W2053018237C153911025 @default.
- W2053018237 hasConceptScore W2053018237C159047783 @default.
- W2053018237 hasConceptScore W2053018237C17757408 @default.
- W2053018237 hasConceptScore W2053018237C22744801 @default.
- W2053018237 hasConceptScore W2053018237C2522874641 @default.
- W2053018237 hasConceptScore W2053018237C2777542201 @default.
- W2053018237 hasConceptScore W2053018237C2780593183 @default.
- W2053018237 hasConceptScore W2053018237C30481170 @default.
- W2053018237 hasConceptScore W2053018237C49105822 @default.
- W2053018237 hasConceptScore W2053018237C54355233 @default.