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- W2053103690 abstract "Partially melted DNA is known to exhibit an abrupt decrease of electrophoretic mobility in a gel. Although this is the main phenomenon exploited in TGGE/DGGE (temperature gradient gel electrophoresis/denaturing gradient gel electrophoresis), not much is known about the physical processes responsible for the blocking. While there is a commonly used formula for the reduced mobility based on the theory of branched polymers, it does not discriminate between denatured domains bounded on one (split end) or two sides (bubble). To better understand how the blocking occurs in both of these cases, a coarse‐grained model of DNA gel electrophoresis is simulated using Langevin Dynamics. The simulations reveal that the low‐field mobility is much more sensitive to denatured domains located at the ends of a DNA fragment. A denatured domain occurring at the center of a fragment indeed reduces the mobility, but at a much lower rate." @default.
- W2053103690 created "2016-06-24" @default.
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- W2053103690 date "2012-05-01" @default.
- W2053103690 modified "2023-09-24" @default.
- W2053103690 title "Electrophoretic mobility of partially denatured DNA in a gel: Qualitative and semiquantitative differences between bubbles and split ends" @default.
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- W2053103690 doi "https://doi.org/10.1002/elps.201200097" @default.
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