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• W2053108757 abstract "Purpose.: Human embryonic stem cell–derived RPE (hES-RPE) transplantation is a promising therapy for atrophic age-related macular degeneration (AMD); however, future therapeutic approaches may consider co-transplantation of hES-RPE with retinal progenitor cells (RPCs) as a replacement source for lost photoreceptors. The purpose of this study was to determine the effect of polarization of hES-RPE monolayers on their ability to promote survival of RPCs. Methods.: The hES-3 cell line was used for derivation of RPE. Polarization of hES-RPE was achieved by prolonged growth on permeable inserts. RPCs were isolated from 16- to 18-week-gestation human fetal eyes. ELISA was performed to measure pigment epithelium–derived factor (PEDF) levels from conditioned media. Results.: Pigmented RPE-like cells appeared as early as 4 weeks in culture and were subcultured at 8 weeks. Differentiated hES-RPE had a normal chromosomal karyotype. Phenotypically polarized hES-RPE cells showed expression of RPE-specific genes. Polarized hES-RPE showed prominent expression of PEDF in apical cytoplasm and a marked increase in secretion of PEDF into the medium compared with nonpolarized culture. RPCs grown in the presence of supernatants from polarized hES-RPE showed enhanced survival, which was ablated by the presence of anti-PEDF antibody. Conclusions.: hES-3 cells can be differentiated into functionally polarized hES-RPE cells that exhibit characteristics similar to those of native RPE. On polarization, hES-RPE cells secrete high levels of PEDF that can support RPC survival. These experiments suggest that polarization of hES-RPE would be an important feature for promotion of RPC survival in future cell therapy for atrophic AMD." @default.
• W2053108757 created "2016-06-24" @default.
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• W2053108757 date "2011-03-21" @default.
• W2053108757 modified "2023-09-23" @default.
• W2053108757 title "Polarized Secretion of PEDF from Human Embryonic Stem Cell–Derived RPE Promotes Retinal Progenitor Cell Survival" @default.
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• W2053108757 doi "https://doi.org/10.1167/iovs.10-6413" @default.
• W2053108757 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4183377" @default.
• W2053108757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21087957" @default.
• W2053108757 hasPublicationYear "2011" @default.
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