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- W2053133067 abstract "We developed an effective protocol for generating high-purity heteroduplexes via annealing single-stranded DNAs (ssDNAs) derived from plasmid DNA by asymmetric polymerase chain reaction (A-PCR). With the addition of dimethyl sulfoxide, a one-step A-PCR procedure can generate ssDNAs stably at a range of reaction temperatures. Several annealing buffers can anneal two ssDNAs into heteroduplexes effectively. We further developed a simple strategy to create d(GATC) hemimethylated heteroduplexes by annealing fully methylated homoduplexes in the presence of excessive unmethylated ssDNAs. The constructed heteroduplexes have been well tested as substrates for mismatch repair in Escherichia coli and, thus, can be used in various biotechnology applications." @default.
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- W2053133067 date "2015-04-01" @default.
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- W2053133067 title "Assembling long heteroduplexes by asymmetric polymerase chain reaction and annealing the resulting single-stranded DNAs" @default.
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- W2053133067 doi "https://doi.org/10.1016/j.ab.2014.12.017" @default.
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