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• W2053135095 abstract "ABSTRACT Release of NAD+ during preparation of murine lymphocytes causes enzymatic ADP-ribosylation of cell-surface proteins on T cells, catalyzed by toxin-related ecto-ADP-ribosyltransferase, ARTC2. ADP-riboslyation activates the cytolytic P2X7 ion channel and affects, in particular, the vitality and function of Tregs and NKT cells. Here, we describe a simple method—injection of an ARTC2-blocking nanobody—to greatly improve Treg and NKT cell vitality and to preserve their function during in vitro assays and in adoptive-transfer experiments. Moreover, we present a method for the sorting of functional, primary NKT cells, based on coexpression of ARTC2 and NK1.1. Our results pave the way for the efficient ex vivo proliferation of Tregs and NKT cells and for new experimental and therapeutic uses of these important regulatory cells." @default.
• W2053135095 created "2016-06-24" @default.
• W2053135095 creator A5025732687 @default.
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• W2053135095 date "2013-11-08" @default.
• W2053135095 modified "2023-10-16" @default.
• W2053135095 title "Technical Advance: A new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells" @default.
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• W2053135095 doi "https://doi.org/10.1189/jlb.0713407" @default.
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