Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053164365> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2053164365 endingPage "1691" @default.
- W2053164365 startingPage "1685" @default.
- W2053164365 abstract "Endonuclease V of bacteriophage T4 binds to UV-irradiated deoxyribonucleic acid (DNA) but not to unirradiated DNA. We have developed an assay to detect this binding, based on the retention of enzyme--DNA complexes on nitrocellulose filters. The amount of complex retained, ascertained by using radioactive DNA, is a measure of T4 endonuclease V activity. The assay is simple, rapid, and specific, which makes it useful for detecting T4 endonuclease V activity both in crude lysates and in purified preparations. We have used it to monitor enzyme activity during purification and to study binding of the enzyme to DNA under conditions that minimize the ability of the enzyme to nick DNA. From our data we conclude that (1) T4 endonuclease V binds to UV-irradiated DNA but not to DNA that has been previously incised by the endonuclease, (2) equilibrium between the free and complexed form of the enzyme is attained under our reaction conditions, (3) dissociation of enzyme--DNA complexes is retarded by sodium cyanide, and (4) retention of enzyme--DNA complexes on nitrocellulose filters is enhanced by high concentrations of saline--citrate." @default.
- W2053164365 created "2016-06-24" @default.
- W2053164365 creator A5014422448 @default.
- W2053164365 creator A5016425613 @default.
- W2053164365 creator A5029872695 @default.
- W2053164365 date "1980-04-15" @default.
- W2053164365 modified "2023-09-23" @default.
- W2053164365 title "Binding of T4 endonuclease V to deoxyribonucleic acid irradiated with ultraviolet light" @default.
- W2053164365 cites W1492044140 @default.
- W2053164365 cites W1572168062 @default.
- W2053164365 cites W1573008071 @default.
- W2053164365 cites W1639307728 @default.
- W2053164365 cites W1858120728 @default.
- W2053164365 cites W2001918964 @default.
- W2053164365 cites W2006046073 @default.
- W2053164365 cites W2012108545 @default.
- W2053164365 cites W2017927310 @default.
- W2053164365 cites W2021753888 @default.
- W2053164365 cites W2024976563 @default.
- W2053164365 cites W2036350293 @default.
- W2053164365 cites W2038124483 @default.
- W2053164365 cites W2040253399 @default.
- W2053164365 cites W2047441587 @default.
- W2053164365 cites W2060294859 @default.
- W2053164365 cites W2076433746 @default.
- W2053164365 cites W2089292706 @default.
- W2053164365 cites W2095055244 @default.
- W2053164365 cites W2158300746 @default.
- W2053164365 cites W2161095945 @default.
- W2053164365 cites W2323336772 @default.
- W2053164365 cites W4238085178 @default.
- W2053164365 cites W4246643285 @default.
- W2053164365 cites W4296765338 @default.
- W2053164365 doi "https://doi.org/10.1021/bi00549a026" @default.
- W2053164365 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6246930" @default.
- W2053164365 hasPublicationYear "1980" @default.
- W2053164365 type Work @default.
- W2053164365 sameAs 2053164365 @default.
- W2053164365 citedByCount "32" @default.
- W2053164365 countsByYear W20531643652016 @default.
- W2053164365 crossrefType "journal-article" @default.
- W2053164365 hasAuthorship W2053164365A5014422448 @default.
- W2053164365 hasAuthorship W2053164365A5016425613 @default.
- W2053164365 hasAuthorship W2053164365A5029872695 @default.
- W2053164365 hasConcept C153911025 @default.
- W2053164365 hasConcept C181199279 @default.
- W2053164365 hasConcept C185592680 @default.
- W2053164365 hasConcept C2777028655 @default.
- W2053164365 hasConcept C2778954975 @default.
- W2053164365 hasConcept C28212737 @default.
- W2053164365 hasConcept C2987116853 @default.
- W2053164365 hasConcept C41625074 @default.
- W2053164365 hasConcept C552990157 @default.
- W2053164365 hasConcept C55493867 @default.
- W2053164365 hasConcept C75473681 @default.
- W2053164365 hasConcept C86803240 @default.
- W2053164365 hasConceptScore W2053164365C153911025 @default.
- W2053164365 hasConceptScore W2053164365C181199279 @default.
- W2053164365 hasConceptScore W2053164365C185592680 @default.
- W2053164365 hasConceptScore W2053164365C2777028655 @default.
- W2053164365 hasConceptScore W2053164365C2778954975 @default.
- W2053164365 hasConceptScore W2053164365C28212737 @default.
- W2053164365 hasConceptScore W2053164365C2987116853 @default.
- W2053164365 hasConceptScore W2053164365C41625074 @default.
- W2053164365 hasConceptScore W2053164365C552990157 @default.
- W2053164365 hasConceptScore W2053164365C55493867 @default.
- W2053164365 hasConceptScore W2053164365C75473681 @default.
- W2053164365 hasConceptScore W2053164365C86803240 @default.
- W2053164365 hasIssue "8" @default.
- W2053164365 hasLocation W20531643651 @default.
- W2053164365 hasLocation W20531643652 @default.
- W2053164365 hasOpenAccess W2053164365 @default.
- W2053164365 hasPrimaryLocation W20531643651 @default.
- W2053164365 hasRelatedWork W1499152451 @default.
- W2053164365 hasRelatedWork W1548333395 @default.
- W2053164365 hasRelatedWork W1635821397 @default.
- W2053164365 hasRelatedWork W1964156205 @default.
- W2053164365 hasRelatedWork W1965890903 @default.
- W2053164365 hasRelatedWork W1988259208 @default.
- W2053164365 hasRelatedWork W2007214131 @default.
- W2053164365 hasRelatedWork W2010372900 @default.
- W2053164365 hasRelatedWork W2025480991 @default.
- W2053164365 hasRelatedWork W2410650298 @default.
- W2053164365 hasVolume "19" @default.
- W2053164365 isParatext "false" @default.
- W2053164365 isRetracted "false" @default.
- W2053164365 magId "2053164365" @default.
- W2053164365 workType "article" @default.