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- W2053172534 abstract "A method for the preparation of immunotoxins (ITs) containing one and two molecules of deglycosylated ricin A chain (dgA) bound to each molecule of anti-human CD22 mouse IgG1 monoclonal antibody is described. The cross-linking of antibody and dgA molecules was accomplished with N-succinimidyl-oxycarbonyl-α-methyl-(2-pyridyldithio). The two ITs were purified by successive chromatographies on Blue-Sepharose, Sephacryl S-200, Blue-Sepharose with NaCl gradient and Sephacryl S-300. The IT with two dgAs was seven-fold more cytotoxic to CD22+ Daudi cells than that containing one molecule of dgA." @default.
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- W2053172534 date "1993-11-01" @default.
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- W2053172534 title "Purification and properties of immunotoxins containing one vs. two deglycosylated ricin A chains" @default.
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- W2053172534 doi "https://doi.org/10.1016/0022-1759(93)90335-5" @default.
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