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• W2053176568 startingPage "104" @default.
• W2053176568 abstract "Abstract Type IV secretion systems (T4 SS ) can mediate the translocation of bacterial virulence proteins into host cells. The plant pathogen A grobacterium tumefaciens uses a T4 SS to deliver a VirD2‐single stranded DNA complex as well as the virulence proteins VirD5, VirE2, VirE3, and VirF into host cells so that these become genetically transformed. Besides plant cells, yeast and fungi can efficiently be transformed by A grobacterium . Translocation of virulence proteins by the T4 SS has so far only been shown indirectly by genetic approaches. Here we report the direct visualization of VirE2 protein translocation by using bimolecular fluorescence complementation (Bi FC ) and Split GFP visualization strategies. To this end, we cocultivated A grobacterium strains expressing VirE2 tagged with one part of a fluorescent protein with host cells expressing the complementary part, either fused to VirE2 (for Bi FC ) or not (Split GFP ). Fluorescent filaments became visible in recipient cells 20–25 h after the start of the cocultivation indicative of VirE2 protein translocation. Evidence was obtained that filament formation was due to the association of VirE2 with the microtubuli." @default.
• W2053176568 created "2016-06-24" @default.
• W2053176568 creator A5025062195 @default.
• W2053176568 creator A5074960830 @default.
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• W2053176568 date "2013-12-27" @default.
• W2053176568 modified "2023-10-05" @default.
• W2053176568 title "Visualization of VirE2 protein translocation by the<i><scp>A</scp>grobacterium</i>type IV secretion system into host cells" @default.
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• W2053176568 doi "https://doi.org/10.1002/mbo3.152" @default.
• W2053176568 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3937733" @default.
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• W2053176568 hasPublicationYear "2013" @default.
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