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• W2053178864 abstract "The dimorphic fission yeast Schizosaccharomyces japonicus has proved to be an excellent experimental model for the investigation of the eukaryotic cell. Here we show that it has a haplontic life cycle, in which the diploid phase is confined to the zygote. To make it amenable to genetic and molecular analysis, we generated genetic markers and cloned a genomic sequence which acts as ars when integrated into a plasmid. Diploids suitable for testing complementation and recombination between markers can be formed by protoplast fusion. The complementation tests and the recombination frequencies determined in octads of spores identified 28 non-allelic groups (genes) of mutations of the auxotrophic and mycelium-negative mutants. Two groups of linked markers were also identified. The cloned fragment, which expresses ars activity, encodes a putative amino acid sequence highly similar to a conserved domain of proteins Cut1 (Schizosaccharomyces pombe), BimB (Aspergillus nidulans) and Esp1 (Saccharomyces cerevisiae)." @default.
• W2053178864 created "2016-06-24" @default.
• W2053178864 creator A5022768371 @default.
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• W2053178864 date "2002-01-01" @default.
• W2053178864 modified "2023-10-15" @default.
• W2053178864 title "Marker construction and cloning of acut1-like sequence with ARS activity in the fission yeastSchizosaccharomyces japonicus" @default.
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• W2053178864 doi "https://doi.org/10.1002/yea.853" @default.
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