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• W2053193478 abstract "The endoglucanase (E1) from Acidothermus cellulolyticus has been used extensively in cellulase research. The goal of this work was to produce high levels of this enzyme in a system that facilitates purification. A codon-optimized synthetic gene for A. cellulolyticus E1 with a C-terminal histidine tag was cloned into the genome of Pichia pastoris. Strain KM71H expressed the most enzyme, with a yield of 550mg/L culture supernatant. The temperature optimum (80°C) and pH optimum (5.1) of the purified enzyme agree with previously determined values for the enzyme produced in other systems. Michaelis-Menten kinetic parameters were determined, using a fluorescent substrate (methylumbelliferyl-β-d-cellobioside) at various temperatures. This thermostable enzyme can be used in future cellulosic biofuels-related research." @default.
• W2053193478 created "2016-06-24" @default.
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• W2053193478 date "2011-06-01" @default.
• W2053193478 modified "2023-10-07" @default.
• W2053193478 title "Production and characterization of Acidothermus cellulolyticus endoglucanase in Pichia pastoris" @default.
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• W2053193478 doi "https://doi.org/10.1016/j.pep.2011.01.006" @default.
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